The Cytotoxicity of Microglass Fibers on Alveolar Macrophages of Fischer 344 Rats Evaluated by Cell Magnetometry, Cytochemisry and Morphology

Shinji, Hisako; Watanabe, Mitsuyasu; Kudo, Yuichiro; Niitsuya, Masato; Tsunoda, Masashi; Sakurai, Toshihiko; Sakai, Yasuhiro; Kotani, Masahiro; Aizawa, Yoshifusa

doi:10.1265/ehpm.10.111

Cited by 3 publications

(3 citation statements)

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“…Intracellular rotation of spherical iron oxide microspheres in macrophages has been shown to be dependent on cytoskeletal integrity, and the rotation rate decreases when the cells are exposed to toxic nanoparticles such as asbestos fibers and microglass fibers. 19 However, the rotation of the fibers themselves has not been studied. The silver nanobelts we synthesized above have different LSPR modes for light polarized along the The Journal of Physical Chemistry Letters LETTER different cross-section axes, enabling roll orientation to be determined from the resonant scattering color and intensity in dark-field microscopy.…”

Section: Section: Nanoparticles and Nanostructuresmentioning

confidence: 99%

“…Particles with high aspect ratio can be especially toxic (e.g., asbestos fibers), and studying their internal motions can help elucidate the toxic mechanisms. Intracellular rotation of spherical iron oxide microspheres in macrophages has been shown to be dependent on cytoskeletal integrity, and the rotation rate decreases when the cells are exposed to toxic nanoparticles such as asbestos fibers and microglass fibers . However, the rotation of the fibers themselves has not been studied.…”

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confidence: 99%

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Plasmonic Silver Nanobelts via Citrate Reduction in the Presence of HCl and their Orientation-Dependent Scattering Properties

Yang

Nguyen

Chen

et al. 2011

J. Phys. Chem. Lett.

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Silver nanobelts were synthesized without any external seeds or templates using a facile hydrothermal route via citrate reduction in the presence of hydrochloric acid. Both H+ and Cl– ions in HCl are important for the generation of nanobelts. The morphology can be controlled by varying the concentration of HCl and citrate. The obtained nanobelts have 3D orientation-dependent optical properties. By tracking the transportation and rotation of the nanobelts in cells or other biosystems, useful information related to the physical or chemical surroundings may be obtained.

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Section: Section: Nanoparticles and Nanostructuresmentioning

confidence: 99%

mentioning

confidence: 99%

Plasmonic Silver Nanobelts via Citrate Reduction in the Presence of HCl and their Orientation-Dependent Scattering Properties

Yang

Nguyen

Chen

et al. 2011

J. Phys. Chem. Lett.

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“…To date, we have evaluated the cytotoxicity of chrysotile, a type of asbestos, as well as RW and other man-made vitreous fibers (MMVFs), by cell magnetometry that was originally devised in our laboratory [ 8 - 12 ]. This method determines cytoskeleton-dependent functions of macrophages, which play an important role in phagocytosis, to evaluate the degree of injury caused on macrophages.…”

Section: Introductionmentioning

confidence: 99%

Effects of rock wool on the lungs evaluated by magnetometry and biopersistence test

Kudo

Kotani

Tomita

et al. 2009

Journal of Occupational Medicine and Toxicology

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Background: Asbestos has been reported to cause pulmonary fibrosis, and its use has been banned all over the world. The related industries are facing an urgent need to develop a safer fibrous substance. Rock wool (RW), a kind of asbestos substitute, is widely used in the construction industry. In order to evaluate the safety of RW, we performed a nose-only inhalation exposure study in rats. After one-month observation period, the potential of RW fibers to cause pulmonary toxicity was evaluated based on lung magnetometry findings, pulmonary biopersistence, and pneumopathology.

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Magnetometric Evaluation of the Effects of Man-Made Mineral Fibers on the Function of Macrophages Using the Macrophage Cell Line RAW 264.7

et al. 2007

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The toxic effects of man-made mineral fibers (MMMFs) have been evaluated by cell magnetometry using alveolar macrophages (AMs). Recently, on the other hand, the murine macrophage cell line, RAW 264.7, became available and has been used as an in vitro model of AMs. The objective of this study was to determine whether or not cell magnetometry using RAW 264.7 cells can be used to evaluate the toxic effects of MMMFs. RAW 264.7 cells were exposed to one of the MMMFs, potassium octatitanate (PT) or silicon carbide whisker (SiC) at 0, 20, 40 and 60 µ µ µ µ µg/ml, or chrysotile as a positive control at 0, 15, 20 and 25 µ µ µ µ µg/ml. The toxic effects of fibers were evaluated by cell magnetometry and LDH assay. For this comparison, AMs were also exposed to chrysotile fibers (CF). In the RAW 264.7 cells exposed to PT 20, 40, 60 or SiC 20, 40, 60, CF 15, 20 and 25 µ µ µ µ µg/ml, significant delayed relaxation were observed compared with the respective control. In the LDH assay, significant increases in LDH in the supernatant of the cells exposed to PT 20, 40, 60, SiC 20, 40, 60 and CF 15, 20, 25 µ µ µ µ µg/ml were observed. In AMs exposed to CF 20, 25 µ µ µ µ µg/ml significant delayed relaxation and significant increases in LDH compared with the control were observed. The levels of MMMFs that induced significant differences were similar for cell magnetometry and LDH. The levels of CF that induced significant differences in cell magnetometry and LDH were identical for RAW 264.7 cells and AMs. Our results suggest that cell magnetometry using RAW 264.7 cells is adequate to evaluate the cytotoxicity of exposure to MMMFs.

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The Cytotoxicity of Microglass Fibers on Alveolar Macrophages of Fischer 344 Rats Evaluated by Cell Magnetometry, Cytochemisry and Morphology

Cited by 3 publications

References 24 publications

Plasmonic Silver Nanobelts via Citrate Reduction in the Presence of HCl and their Orientation-Dependent Scattering Properties

Plasmonic Silver Nanobelts via Citrate Reduction in the Presence of HCl and their Orientation-Dependent Scattering Properties

Effects of rock wool on the lungs evaluated by magnetometry and biopersistence test

Magnetometric Evaluation of the Effects of Man-Made Mineral Fibers on the Function of Macrophages Using the Macrophage Cell Line RAW 264.7

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