37Until now, sedimentary DNA (sedDNA) studies have only focused on cold and temperate 38 regions were DNA is relatively well preserved. Consequently, the tropics, where 39 vegetation is hyperdiverse and natural archives are rare, have been neglected and 40 deserve attention. In this study, we used next-generation sequencing to barcode sedDNA 41 from Lake Sele, localized in the tropical lowlands of Benin (Africa), and compared the 42 taxonomic diversity detected by DNA analyses with pollen assemblages. Plant sedDNA 43 was successfully amplified from 33 of the 34 successfully extracted samples. In total, 43 44 taxa were identified along the 5,000 years spanned by the sediment: 22 taxa were 45 identified at the family level and 21 at the genus level. The plant diversity recovered 46 through sedDNA from Lake Sele showed a specific local signal and limited overlapping 47 with pollen. Introduced plants, grown and cultivated close to the water, such as sweet 48 potato, were also well recorded by sedDNA. It appears, therefore, to be a promising 49 approach to studying past diversity in tropical regions, and could help in tracking the 50 introduction and history of agriculture. This is the first time this method has been used 51