The degradation and performance of electrospun supramolecular vascular scaffolds examined upon in vitro enzymatic exposure

Haaften, Eline E. van; Duijvelshoff, Renée; Ippel, Bastiaan D.; Söntjens, Serge H. M.; Houtem, Michel H. C. J. van; Janssen, Henk M.; Smits, Anthal I.P.M.; Kurniawan, Nicholas A.; Dankers, Patricia Y. W.; Bouten, Cvc Carlijn

doi:10.1016/j.actbio.2019.05.037

Cited by 27 publications

(26 citation statements)

References 39 publications

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“…As the scaffold degrades and neo-tissue develops, hemodynamic loading on the scaffold-populating cells will change in a time-dependent fashion. 1,18 This mechanical loading has been shown to directly affect biomaterial degradation kinetics. 19 In addition, hemodynamic loads have been identified as important determinants of tissue regeneration and are known to dictate the structure-function properties of the newly formed tissue.…”

Section: Introductionmentioning

confidence: 99%

Hemodynamic loads distinctively impact the secretory profile of biomaterial-activated macrophages – implications forin situvascular tissue engineering

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Hemodynamic loads distinctively impact the secretory profile of biomaterial-activated macrophages – implications forin situvascular tissue engineering

et al. 2020

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“…Previous in situ tissue engineering studies have shown that synthetic grafts based on supramolecular materials are able to perform as regenerative resorbable grafts, which enable attraction of endogenous cells and consecutive matrix production in situ (Talacua et al, 2015;Muylaert et al, 2016;Van Almen et al, 2016;van Haaften et al, 2019). However, kinking in the flexible lumen, as well as changes in the wall are complications that cause low patency of the vascular grafts.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, supramolecular polymers based on bisurea (BU) and 2-ureido-4[1H]-pyrimidinone (UPy)-modified supramolecular polymers were used to develop electrospun microporous vascular grafts that finally are proposed to be biocompatible and fully resorbable (Figure 1A;Van Almen et al, 2016;Kluin et al, 2017;van Haaften et al, 2017;Ippel et al, 2019;van Haaften et al, 2019). These supramolecular polymers have been identified as promising candidates to synthetically mimic the complexity of extracellular matrix (Goor et al, 2017;Diba et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…The unique and adaptable mechanical properties of BUmodified polymer arise from the molecular composition, where BU hard-blocks with ability of crystallization through hydrogenbonding are alternated with a rubbery PC soft-block which ensures biocompatibility and slow degradation ( Figure 1B; Appel et al, 2011;van Haaften et al, 2019). The well-defined BU hardblocks within thermoplastic elastomers enhance the mechanical properties, which is evident from the high tensile strengths (Versteegen et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Anti-kinking Designs for Vascular Grafts Based on Supramolecular Materials

Dongen

Szymczyk

et al. 2020

Front. Mater.

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Synthetic vascular grafts to be applied as access grafts for hemodialysis often require anti-kinking properties. Previously, electrospun microporous vascular implants based on synthetic supramolecular materials have been shown to perform adequately as resorbable grafts due to the microstructures, thereby enabling attraction of endogenous cells and consecutive matrix production in situ. Here, we use supramolecular materials based on hydrogen bonding interactions between bisurea (BU) or 2-ureido-4[1H]-pyrimidinones (UPy) to produce microporous anti-kinking tubular structures by combining solution electrospinning with 3D printing. A custom-made rational axis for 3D printing was developed to produce controlled tubular structures with freedom in design in order to print complex tubular architectures without supporting structures. Two different tubular grafts were developed, both composed of a three-layered design with a 3D printed spiral sandwiched in between luminal and adventitial electrospun layers. One tubular scaffold was composed of BU-polycarbonate electrospun layers with 3D printed polycaprolactone (PCL) strands in between for dimensional stability, and the other graft fully consisted of supramolecular polymers, using chain-extended UPy-PCL as electrospun layers and a bifunctional UPy-PCL for 3D printing. Both grafts, with a 3D printed spiral, demonstrated a reproducible dimensional stability and anti-kinking behavior under bending stresses.

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“… 22 , 67 , 72 , 75 , 77 An added benefit of using PCL is that the degradation mechanisms are well-described, including variants containing Upy or BU units, which allows for fine-tuning of degradation properties. 60 , 67 , 68 , 81 , 86 Considering the (well-known) properties of SES and PCL, it is no surprise these are currently the most used in renal tubule modeling. We expect the SES-PCL scaffolds will remain a viable option for renal application, but considerable work is still required to identify the optimal fiber range, material stiffness, and degradation properties for this specific application.…”

Section: Critical Aspects To Consider When Designing a Synthetic Proximal Tubulementioning

confidence: 99%

Renal Biology Driven Macro- and Microscale Design Strategies for Creating an Artificial Proximal Tubule Using Fiber-Based Technologies

Vermue

Begum

Castilho

et al. 2021

ACS Biomater. Sci. Eng.

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Chronic kidney disease affects one in six people worldwide. Due to the scarcity of donor kidneys and the complications associated with hemodialysis (HD), a cell-based bioartificial kidney (BAK) device is desired. One of the shortcomings of HD is the lack of active transport of solutes that would normally be performed by membrane transporters in kidney epithelial cells. Specifically, proximal tubule (PT) epithelial cells play a major role in the active transport of metabolic waste products. Therefore, a BAK containing an artificial PT to actively transport solutes between the blood and the filtrate could provide major therapeutic advances. Creating such an artificial PT requires a biocompatible tubular structure which supports the adhesion and function of PT-specific epithelial cells. Ideally, this scaffold should structurally replicate the natural PT basement membrane which consists mainly of collagen fibers. Fiber-based technologies such as electrospinning are therefore especially promising for PT scaffold manufacturing. This review discusses the use of electrospinning technologies to generate an artificial PT scaffold for ex vivo / in vivo cellularization. We offer a comparison of currently available electrospinning technologies and outline the desired scaffold properties required to serve as a PT scaffold. Discussed also are the potential technologies that may converge in the future, enabling the effective and biomimetic incorporation of synthetic PTs in to BAK devices and beyond.

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The degradation and performance of electrospun supramolecular vascular scaffolds examined upon in vitro enzymatic exposure

Cited by 27 publications

References 39 publications

Hemodynamic loads distinctively impact the secretory profile of biomaterial-activated macrophages – implications forin situvascular tissue engineering

Hemodynamic loads distinctively impact the secretory profile of biomaterial-activated macrophages – implications forin situvascular tissue engineering

Optimization of Anti-kinking Designs for Vascular Grafts Based on Supramolecular Materials

Renal Biology Driven Macro- and Microscale Design Strategies for Creating an Artificial Proximal Tubule Using Fiber-Based Technologies

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