Objective-During pregnancy, fetal trophoblast disrupt endothelial cell and vascular smooth muscle cell (VSMC) interactions in spiral arteries of the maternal decidua to enable increased nutritional and oxygen delivery to the fetus. Little is known regarding this transformation because of difficulties of studying human pregnancy in vivo. This study investigated how trophoblast-secreted factors affect the interactions of vascular cells and the differentiation status of VSMC during spiral arteries remodeling using 3-dimensional vascular spheroid coculture. Methods and Results-Endothelial cell and VSMC were cocultured in hanging droplets to form spheroids representing an inverted vessel lumen. Control or conditioned media from an extravillous trophoblast (EVT) cell line was incubated with vascular spheroids for 24 hours. Spheroid RNA was then analyzed by Illumina Sentrix BeadChip array. Spheroids incubated with EVT conditioned medium showed significant up/downregulation of 101 genes (>1.5-fold; P<0.05), including an upregulation of C-X-C motif chemokine 10 (IP-10). C-X-C motif chemokine 10 expression was confirmed by qualitative real-time PCR and Western blot analysis of spheroids, and immunohistochemistry of first trimester decidua and ex vivo dissected nonplacental bed spiral arteries. EVT conditioned medium reduced VSMC expression of differentiation markers, and both EVT conditioned medium and C-X-C motif chemokine 10 increased motility of VSMC indicating dedifferentiation of VSMC. Conclusion-EVT-induced

Until recently, bioprinting was largely limited to highly interdisciplinary research teams, as the process requires significant input from specialists in the fields of materials science, engineering, and cell biology. With the advent of commercially available high‐performance bioprinters, the field has become accessible to a wider range of research groups, who can now buy the hardware off the shelf instead of having to build it from scratch. As a result, bioprinting has rapidly expanded to address a wide array of research foci, which include organotypic in vitro models, complex engineered tissues, and even bioprinted microbial systems. Moreover, in the early days, the range of suitable bioinks was limited. Now, there is a plethora of viable options to suit many cell phenotypes. This rapidly evolving dynamic environment creates endless opportunities for scientists to design and construct highly complex biological systems. However, this scientific diversity presents its own set of challenges, such as defining standardized protocols for characterizing bioprinted structures, which is essential for eventual organ replacement. In this progress report, the current state‐of‐the‐art in the field of bioprinting is discussed, with a special emphasis on recent hardware developments, bioprinting for regenerative medicine, and late‐breaking nontraditional topics.

Smart biomaterials with an inherent capacity to elicit specific behaviors in lieu of biological prompts would be advantageous for regenerative medicine applications. In this work, we employ an electrospinning technique to model the in vivo nanofibrous extracellular matrix (ECM) of cartilage using a chondroinductive cellulose and silk polymer blend (75:25 ratio). This natural polymer composite is directly electrospun for the first time, into nanofibers without post-spun treatment, using a trifluoroacetic acid and acetic acid cosolvent system. Biocompatibility of the composite nanofibres with human mesenchymal stem cells (hMSCs) is demonstrated and its inherent capacity to direct chondrogenic stem cell differentiation, in the absence of stimulating growth factors, is confirmed. This chondrogenic stimulation could be countered biochemically using fibroblast growth factor-2, a growth factor used to enhance the proliferation of hMSCs. Furthermore, the potential mechanisms driving this chondroinduction at the cell-biomaterial interface is investigated. Composite substrates are fabricated as two-dimensional film surfaces and cultured with hMSCs in the presence of chemicals that interfere with their biochemical and mechanical signaling pathways. Preventing substrate surface elasticity transmission resulted in a significant downregulation of chondrogenic gene expression. Interference with the classical chondrogenic Smad2/3 phosphorylation pathway did not impact chondrogenesis. The results highlight the importance of substrate mechanical elasticity on hMSCs chondroinduction and its independence to known chondrogenic biochemical pathways. The newly fabricated scaffolds provide the foundation for designing a robust, self-inductive, and cost-effective biomimetic biomaterial for cartilage tissue engineering.

Groundwater is an essential and valuable natural source of drinking water. But sometime ground water contains different types of chemical or biological substance which make water unsuitable for consumption. Quality of the ground water varies in different location. The recent study emphasized on monitoring the present condition of groundwater in the coastal region of Noakhali. The study area covered 24 different locations of two large Upazila Subarnachar and Kabirhat of Noakhali District. Groundwater quality was examined by analyzing various physicochemical parameters and microbial parameters such as pH, Electrical Conductivity (EC), Total Dissolved Solids (TDS), Salinity, Total Hardness, Potassium, Sulphate, Chloride ions, Total Coliform, Fecal Coliform, and Total Bacterial Viable Count. These parameters were compared with the drinking water quality standards recommended by WHO and Bangladesh local standards. From the analysis, it was observed that pH, Sulphate, Potassium were within the acceptable limit according to WHO and Bangladesh standards. But maximum tube-wells water contains huge amount of TDS (6040 mg/l), EC (1786µs/cm), Salinity (6.8%) and hardness (1050 mg/l), which is not safe for human health. From the correlation studies of the water quality parameters, relatively high positive correlation between some chemical parameters was found. And it signifies a common origin or progressive enrichment of both parameters. The analysis of biological parameters showed the presence of bacteria in many of the water samples. Maximum value of Total coliform found from the groundwater was TNTC and fecal coliform was 3×101 CFU/ml. According to WHO and Bangladesh standard the groundwater of this region is not suitable for drinking. So, some simple primary treatment is needed prior to use this water for drinking purposes and necessary steps should be taken for alternative safe source of drinking water in this region.