The Dehydrogenase Isoenzymes

Clinical Enzymology

“…The extra bands detected in the serum thus appear to be hybrids of the normal H subunit with the abnormal M subunit. Such an occurrence might account for the detection of extra bands in the sera of patients with malignant disease previously reported by Beautyman (1962), Latner (1964) and others.…”

Enzyme assays in malignant disease.

“…LD activity tends to be higher in malignant tissue than in corresponding normal tissue (Meister, 1950) but there is considerable overlap (Latner, 1964). Attempts have been made to associate LD isoenzyme patterns with malignant tumours.…”

“…Attempts have been made to associate LD isoenzyme patterns with malignant tumours. According to Latner (1964), malignant tumours contain predominantly the slower migrating isoenzymes; in the case of carcinoma of the breast, this is supported by Richterich and Burger (1963), and Barnett and Gibson (1964). Serum isoenzyme patterns however do not show any clear cut tumour specificity.…”

Enzymes in Malignant Disease

“…Red Cells Red cell enzyme studies have not proved diagnostically valuable in malignant disease. As association between a high incidence if cancer and genetic red cell LD polymorphism has been reported (Latner, 1964). Other Body Fluids In the examination of other body fluids for the detection of malignancy at specific sites, LD has been studied in effusions, urine, gastric juice and cerebro-spinal fluid and 6-phosphogluconate dehydrogenase (6-PGDH) measured in vaginal fluid.…”

“…These fractions sometimes show abnormal physical properties indicating disturbed protein synthesis (Starkweather and Schoch, 1962). A similarity of tumour LD isoenzymes to that of abnormal red cell isoenzymes in a family with genetic red cell LD polymorphism and a high incidence of cancer may also suggest an occasional genetically determined association between cancer and abnormal LD isoenzyme patterns (Latner, 1964 …”