The DenA/DEN1 Interacting Phosphatase DipA Controls Septa Positioning and Phosphorylation-Dependent Stability of Cytoplasmatic DenA/DEN1 during Fungal Development

Schinke, Josua; Gulko, Miriam Kolog; Christmann, Martin; Valerius, Oliver; Stumpf, Sina K.; Stirz, Margarita; Braus, Gerhard H.

doi:10.1371/journal.pgen.1005949

Cited by 21 publications

(35 citation statements)

References 140 publications

(178 reference statements)

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“…To accomplish this, we constructed two DipA strains: (1) an endogenously tagged DipA with two tandem copies of TagGFP2 at its carboxy-terminus (DipA-GFP) to assess its localization, and (2) a DipA deletion (dipAΔ) strain to assess its cellular function. As previously reported, the dipAΔ strain exhibits perturbed colony growth (Supplementary Figure 1A) [51]. Colonies from the DipA-GFP strain grow normally (Supplementary Figure 1A), suggesting that the GFP tag does not adversely affect protein function.…”

Section: Pxda Interacts With the Dipa Phosphatase On Eessupporting

confidence: 80%

“…Mass spectrometry analysis revealed this band to be the DenA/DEN1 interacting phosphatase (DipA). DipA interacts with and regulates the stability of the deneddylase DenA/DEN1 on motile puncta in the cytoplasm, a process important for fungal asexual development [51].…”

Section: Pxda Interacts With the Dipa Phosphatase On Eesmentioning

confidence: 99%

“…DipA requires PxdA to associate with EEs. DipA is a phosphatase whose function was previously linked to regulating the phosphorylation status and degradation of DenA/Den1, a protein involved in asexual spore formation [51,53]. DenA is co-transported with DipA [51].…”

Section: Dipa Is a Regulator Of Peroxisome Hitchhikingmentioning

confidence: 99%

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PxdA interacts with the DipA phosphatase to regulate endosomal hitchhiking of peroxisomes

Salogiannis

Christensen

Aguilar-Maldonado

et al. 2020

Preprint

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The canonical mechanism of microtubule-based movement uses adaptor proteins to link cargos to the molecular motors dynein and kinesin. Recently, an alternative mechanism known as "hitchhiking" was discovered: ribonucleoproteins, peroxisomes, lipid droplets, and endoplasmic reticulum achieve motility by hitching a ride on motile early endosomes, rather than attaching directly to a motor protein. In the filamentous fungus Aspergillus nidulans we identified a molecular linker, PxdA, that associates with early endosomes and is essential for peroxisome hitchhiking. However, the molecular components that interact with PxdA, as well as the regulatory mechanisms that govern hitchhiking, are not understood. Here, we report the identification of two new pxdA alleles, including a point mutation (R2044P). We find that the R2044P mutation disrupts PxdA's ability to associate with early endosomes and consequently reduces peroxisome movement. We also identify the phosphatase DipA as an interaction partner of PxdA. DipA associates with early endosomes in a PxdA-dependent manner, and regulates the movement and distribution of peroxisomes. Finally, we find that PxdA also regulates the distribution of lipid droplets, but not autophagosomes or mitochondria. Our data suggest that PxdA is a central regulator of early endosome-dependent hitchhiking and requires the DipA phosphatase to regulate the movement and distribution of lipid droplets and peroxisomes.

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Section: Pxda Interacts With the Dipa Phosphatase On Eessupporting

confidence: 80%

Section: Pxda Interacts With the Dipa Phosphatase On Eesmentioning

confidence: 99%

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PxdA interacts with the DipA phosphatase to regulate endosomal hitchhiking of peroxisomes

Salogiannis

Christensen

Aguilar-Maldonado

et al. 2020

Preprint

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“…The genetic mechanisms underlying hyphal branching are still elusive in filamentous fungi and it remains to be analysed whether there is a connection between Som1 and the proteins controlling septa positioning. A phenotype of the V. dahliae SOM1 deletion strain is reminiscent of the dipA deletion strain of A. nidulans, which causes increased numbers of septa (Schinke et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Verticillium dahliae transcription factors Som1 and Vta3 control microsclerotia formation and sequential steps of plant root penetration and colonisation to induce disease

et al. 2018

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Summary Verticillium dahliae nuclear transcription factors Som1 and Vta3 can rescue adhesion in a FLO8‐deficient Saccharomyces cerevisiae strain. Som1 and Vta3 induce the expression of the yeast FLO1 and FLO11 genes encoding adhesins. Som1 and Vta3 are sequentially required for root penetration and colonisation of the plant host by V. dahliae. The SOM1 and VTA3 genes were deleted and their functions in fungus‐induced plant pathogenesis were studied using genetic, cell biology, proteomic and plant pathogenicity experiments. Som1 supports fungal adhesion and root penetration and is required earlier than Vta3 in the colonisation of plant root surfaces and tomato plant infection. Som1 controls septa positioning and the size of vacuoles, and subsequently hyphal development including aerial hyphae formation and normal hyphal branching. Som1 and Vta3 control conidiation, microsclerotia formation, and antagonise in oxidative stress responses. The molecular function of Som1 is conserved between the plant pathogen V. dahliae and the opportunistic human pathogen Aspergillus fumigatus. Som1 controls genes for initial steps of plant root penetration, adhesion, oxidative stress response and VTA3 expression to allow subsequent root colonisation. Both Som1 and Vta3 regulate developmental genetic networks required for conidiation, microsclerotia formation and pathogenicity of V. dahliae.

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“…DenA represents a second deneddylase, which interacts at septa with the DenA-interacting protein DipA. This protein controls septum positioning and asexual spore formation (387). F-box proteins involved in fungal development include GrrA, which is required during late fruiting body formation for ascospore maturation (388), or Fbx15, which is required for asexual and sexual development in A. nidulans (389) or for virulence in A. fumigatus (390).…”

Section: Fruiting Body Formationmentioning

confidence: 99%

Fungal Morphogenesis, from the Polarized Growth of Hyphae to Complex Reproduction and Infection Structures

Riquelme

Aguirre

Bartnicki-García

et al. 2018

Microbiol Mol Biol Rev

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288

246

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SUMMARYFilamentous fungi constitute a large group of eukaryotic microorganisms that grow by forming simple tube-like hyphae that are capable of differentiating into more-complex morphological structures and distinct cell types. Hyphae form filamentous networks by extending at their tips while branching in subapical regions. Rapid tip elongation requires massive membrane insertion and extension of the rigid chitin-containing cell wall. This process is sustained by a continuous flow of secretory vesicles that depends on the coordinated action of the microtubule and actin cytoskeletons and the corresponding motors and associated proteins. Vesicles transport cell wall-synthesizing enzymes and accumulate in a special structure, the Spitzenkörper, before traveling further and fusing with the tip membrane. The place of vesicle fusion and growth direction are enabled and defined by the position of the Spitzenkörper, the so-called cell end markers, and other proteins involved in the exocytic process. Also important for tip extension is membrane recycling by endocytosis via early endosomes, which function as multipurpose transport vehicles for mRNA, septins, ribosomes, and peroxisomes. Cell integrity, hyphal branching, and morphogenesis are all processes that are largely dependent on vesicle and cytoskeleton dynamics. When hyphae differentiate structures for asexual or sexual reproduction or to mediate interspecies interactions, the hyphal basic cellular machinery may be reprogrammed through the synthesis of new proteins and/or the modification of protein activity. Although some transcriptional networks involved in such reprogramming of hyphae are well studied in several model filamentous fungi, clear connections between these networks and known determinants of hyphal morphogenesis are yet to be established.

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The DenA/DEN1 Interacting Phosphatase DipA Controls Septa Positioning and Phosphorylation-Dependent Stability of Cytoplasmatic DenA/DEN1 during Fungal Development

Cited by 21 publications

References 140 publications

PxdA interacts with the DipA phosphatase to regulate endosomal hitchhiking of peroxisomes

PxdA interacts with the DipA phosphatase to regulate endosomal hitchhiking of peroxisomes

Verticillium dahliae transcription factors Som1 and Vta3 control microsclerotia formation and sequential steps of plant root penetration and colonisation to induce disease

Fungal Morphogenesis, from the Polarized Growth of Hyphae to Complex Reproduction and Infection Structures

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