The DeoR-type transcriptional regulator SugR acts as a repressor for genes encoding the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in Corynebacterium glutamicum

Gaigalat, Lars; Schlüter, Jan-Philip; Hartmann, Mechthild; Mormann, Sascha; Tauch, Andreas; Pühler, Alfred; Kalinowski, Jörn

doi:10.1186/1471-2199-8-104

Cited by 92 publications

(100 citation statements)

References 61 publications

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“…No PCR product was obtained when reverse transcriptase was omitted. From this experiment we concluded that sugR and cg2116 were organized as an operon, an observation that was also reported by Gaigalat et al (2007).…”

Section: Features Of Sugr Transcription and Expressionsupporting

confidence: 84%

“…Gaigalat et al (2007) suggested that SugR may be able to form complexes with individual binding sites with different sensitivities to sugar phosphates as described for the TrmB regulator of Pyrococcus furiosus (Lee et al, , 2007, and proposed mutational analyses of the SugR-binding sites to clarify this issue. Prior to this analysis, however, SugR binding on its various targets under the same in vitro binding conditions and with a native SugR protein should be analysed.…”

Section: Identification Of Sugr Co-inducersmentioning

confidence: 99%

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Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum

et al. 2009

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Corynebacterium glutamicum is a biotin-auxotrophic bacterium and some strains efficiently produce glutamic acid under biotin-limiting conditions. In an effort to understand C. glutamicum metabolism under biotin limitation, growth of the type strain ATCC 13032 was investigated in batch cultures and a time-course analysis was performed. A transient excretion of organic acids was observed and we focused our attention on lactate synthesis. Lactate synthesis was due to the ldh-encoded l-lactate dehydrogenase (Ldh). Features of Ldh activity and ldh transcription were analysed. The ldh gene was shown to be regulated at the transcriptional level by SugR, a pleiotropic transcriptional repressor also acting on most phosphotransferase system (PTS) genes. Electrophoretic mobility shift assays (EMSAs) and site-directed mutagenesis allowed the identification of the SugR-binding site. Effector studies using EMSAs and analysis of ldh expression in a ptsF mutant revealed fructose 1-phosphate as a highly efficient negative effector of SugR. Fructose 1,6-bisphosphate also affected SugR binding.

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Section: Features Of Sugr Transcription and Expressionsupporting

confidence: 84%

Section: Identification Of Sugr Co-inducersmentioning

confidence: 99%

Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum

et al. 2009

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“…The levels of bglF and bglF2 mRNAs did not increase upon the addition of glucose or fructose. This indicates that the regulatory mechanism of bglF and bglF2 is different from that of pts genes that are controlled by the transcriptional repressor protein SugR (Engels & Wendish, 2007;Gaigalat et al, 2007;Tanaka et al, 2008b). Indeed, we found that bglF and bglF2 mRNA levels did not increase upon the disruption of sugR gene (data not shown).…”

Section: Regulation Of Bglf2 Expression By B-glucosidesmentioning

confidence: 71%

Identification of a second β-glucoside phosphoenolpyruvate : carbohydrate phosphotransferase system in Corynebacterium glutamicum R

et al. 2009

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The phosphoenolpyruvate : carbohydrate phosphotransferase system (PTS) catalyses carbohydrate transport by coupling it to phosphorylation. Previously, we reported a Corynebacterium glutamicum R b-glucoside PTS encoded by bglF. Here we report that C. glutamicum R contains an additional b-glucoside PTS gene, bglF2, organized in a cluster with a putative phospho-b-glucosidase gene, bglA2, and a putative antiterminator, bglG2. While single gene disruption strains of either bglF or bglF2 were able to utilize salicin or arbutin as sole carbon sources, a double disruption strain exhibited defects in utilization of both carbon sources. Expression of both bglF and bglF2 was induced in the presence of either salicin or arbutin, although disruption of bglG2 affected only bglF2 expression. Moreover, in the presence of either salicin or arbutin, glucose completely repressed the expression of bglF but only slightly repressed that of bglF2. We conclude that BglF and BglF2 have a redundant role in b-glucoside transport even though the catabolite repression control of their encoding genes is different. We also show that expression of both bglF and bglF2 requires the general PTS.

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“…However, it is interesting to note that the effects of inactivation of these regulators in any combinations tested on the ramA promoter activity appeared to be unaffected by the carbon source used. Because SugR represses genes involved in sugar uptake and metabolism in the absence of sugar and its repressor activity is inhibited by sugar metabolites (32,35,36,55), the degree of derepression of most SugR target genes in the sugR mutant is higher in the absence of sugar than in its presence. However, the degree of derepression of ramA promoter activity in the sugR mutant in the presence of glucose was comparable to that in the presence of acetate.…”

Section: Discussionmentioning

confidence: 99%

“…For example, expression of the gapA-pgk-tpi operon, encoding the glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, and triose phosphate isomerase, respectively, is coordinately regulated by SugR, RamA, and GlxR (15,31,32). SugR is a global repressor of genes for sugar uptake and metabolism, including phosphotransferase systems, glycolysis, and fermentative lactate dehydrogenase (32)(33)(34)(35)(36)(37). As sugar phosphates, e.g., fructose-1-phosphate and fructose-1,6-bisphosphate, act as negative effectors of SugR, the SugR regulon is derepressed in the presence of sugar.…”

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confidence: 99%

Involvement of Regulatory Interactions among Global Regulators GlxR, SugR, and RamA in Expression of ramA in Corynebacterium glutamicum

et al. 2013

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The central carbon metabolism genes in Corynebacterium glutamicum are under the control of a transcriptional regulatory network composed of several global regulators. It is known that the promoter region of ramA, encoding one of these regulators, interacts with its gene product, RamA, as well as with the two other regulators, GlxR and SugR, in vitro and/or in vivo. Although RamA has been confirmed to repress its own expression, the roles of GlxR and SugR in ramA expression have remained unclear. In this study, we examined the effects of GlxR binding site inactivation on expression of the ramA promoter-lacZ fusion in the genetic background of single and double deletion mutants of sugR and ramA. In the wild-type background, the ramA promoter activity was reduced to undetectable levels by the introduction of mutations into the GlxR binding site but increased by sugR deletion, indicating that GlxR and SugR function as the transcriptional activator and repressor, respectively. The marked repression of ramA promoter activity by the GlxR binding site mutations was largely compensated for by deletions of sugR and/or ramA. Furthermore, ramA promoter activity in the ramA-sugR double mutant was comparable to that in the ramA mutant but was significantly higher than that in the sugR mutant. Taken together, it is likely that the level of ramA expression is dynamically balanced by GlxR-dependent activation and repression by RamA along with SugR in response to perturbation of extracellular and/or intracellular conditions. These findings add multiple regulatory loops to the transcriptional regulatory network model in C. glutamicum.

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The DeoR-type transcriptional regulator SugR acts as a repressor for genes encoding the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in Corynebacterium glutamicum

Cited by 92 publications

References 61 publications

Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum

Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum

Identification of a second β-glucoside phosphoenolpyruvate : carbohydrate phosphotransferase system in Corynebacterium glutamicum R

Involvement of Regulatory Interactions among Global Regulators GlxR, SugR, and RamA in Expression of ramA in Corynebacterium glutamicum

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