The deoxyribonucleoside 5′-triphosphate (dATP and dTTP) pool in phytohemagglutinin-stimulated and non-stimulated human lymphocytes

Influence of Prior Exposure to Zidovudine on Stavudine Phosphorylation In Vivo and Ex Vivo

“…This gave rise to the speculation that prior ZDV treatment may impair subsequent d4T and 3TC phosphorylation. There are alternative explanations for this finding, e.g., the large inter-and intrapatient variability seen in d4T phosphorylation (18,20 In agreement with other studies (8,9,15), the formation of ZDV, d4T, and thymidine triphosphates was enhanced with PHA stimulation. This effect was more marked in healthy volunteers than in HIV-positive patients.…”

Effects of air pollution and smoking on DNA damage of human lymphocytes

“…However, since they have extremely small pools of DNA precursors compared with actively dividing cells [Munch-Petersen et al, 1973], an exogenous supply of deoxyribonucleosides is required for efficient repair [Collins et al, 1995].…”

Biochemical mechanisms in the Killmann experiment: critique of the deoxyuridine suppression test.

“…Cells were collected from cultures by centrifugation at 1,000 g at 4°C for 10 min. Nucleotides were extracted by suspension of the pellet in 1 ml of 60% methanol at -20°C (30). After 20 h at that temperature the supernatant fraction obtained after centrifugation at 1,000 g at 4°C for 10 min was collected and evaporated to dryness under vacuum at room temperature.…”