The Dependence of Nonlinear Electrical Properties of Yeast Suspensions on Temperature and Electrode Shape

Tamura, Keita; Muraji, Masafumi; Tanaka, Kenji; Shirafuji, Tatsuru

doi:10.1541/ieejfms.139.339

Cited by 4 publications

(14 citation statements)

References 5 publications

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“…The yeast used in this paper is Saccharomyces cerevisiae BY4741 [8][9][10][11][12] . The method of yeast culture has been described previously (11).…”

Section: Methodsmentioning

confidence: 99%

“…observed the generation of harmonics from living yeast and argued that it was caused by an enzyme present in the membrane [13][14][15] . A nonlinearity generated near the electrode has been reported 10,12,16 . The generation of the nonlinearity depends on the shape of the electrode and is affected by the intensity and distortion of the electric field near the electrode 10 .…”

Section: Cells Within Living Organisms Transmit Information Through Electrical Signals and Chemical Reactions 12 Characterizing The Elecmentioning

confidence: 98%

“…A method for electrically measuring the state of cells based on their membrane properties has been demonstrated [5][6][7][8][9][10][11][12] . Kell et al found that the response waveform showed a unique pattern when sinusoidal alternating current (AC) voltage with low frequency was applied to a suspension of living yeast [5][6][7] .…”

Section: Cells Within Living Organisms Transmit Information Through Electrical Signals and Chemical Reactions 12 Characterizing The Elecmentioning

confidence: 99%

“…The generation of the nonlinearity depends on the shape of the electrode and is affected by the intensity and distortion of the electric field near the electrode 10 . Although unique factors affecting the generation of harmonics in living yeast, such as the dependence of the generation process on the concentration of yeast in the suspension and the condition of the culture, have been reported, the mechanism underlying this process has not yet been fully elucidated [8][9][10][11][12] .…”

Section: Cells Within Living Organisms Transmit Information Through Electrical Signals and Chemical Reactions 12 Characterizing The Elecmentioning

confidence: 99%

“…Fourier transform (FFT) for 1,024 samples (eight cycles) approximately 90 s after measurement began after using the Hanning window. We calculated the content of the third harmonic according to the fundamental frequency as an index of nonlinearity [9][10][11] . Table 1 (a) shows the third harmonic content at each applied voltage at a set frequency of 14 Hz.…”

Section: We Derived the Harmonic Component Of The Response Waveform By Performing Fastmentioning

confidence: 99%

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Generation of nonlinearity in the electrical response of yeast suspensions

Muraji

Tamura

Tanaka

et al. 2021

Preprint

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The mechanism through which nonlinearity is generated in the response waveform of the electric current obtained by applying alternating current voltage to yeast suspension has not yet been elucidated. In this paper, we showed that the response waveform depends on the applied voltage and frequency. The results showed that distortion (nonlinearity) in the waveform increases as the applied voltage increases and/or the frequency decreases. We suggest a model for the generation of nonlinearity based on the influx of potassium ions into the cell via potassium ion channels and transporters in the membrane due to the applied voltage. Furthermore, we validated this model by simulating an electrical circuit.

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“…The yeast used in this paper is Saccharomyces cerevisiae BY4741 [8][9][10][11][12] . The method of yeast culture has been described previously (11).…”

Section: Methodsmentioning

confidence: 99%

Section: Cells Within Living Organisms Transmit Information Through Electrical Signals and Chemical Reactions 12 Characterizing The Elecmentioning

confidence: 98%

Section: Cells Within Living Organisms Transmit Information Through Electrical Signals and Chemical Reactions 12 Characterizing The Elecmentioning

confidence: 99%

Section: Cells Within Living Organisms Transmit Information Through Electrical Signals and Chemical Reactions 12 Characterizing The Elecmentioning

confidence: 99%

Section: We Derived the Harmonic Component Of The Response Waveform By Performing Fastmentioning

confidence: 99%

See 3 more Smart Citations

Generation of nonlinearity in the electrical response of yeast suspensions

Muraji

Tamura

Tanaka

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Evaluation of the Nonlinear Response of KCl Solution to High-Amplitude Stimulation Using a Four-Point Cylindrical Gold Electrode

Sharifi,

Saviz,

Faraji-Dana

2024

J. Phys. Chem. B

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A four-electrode system and high-voltage amplitude stimulation (with a voltage of more than 1 V) are always used for nonlinear spectroscopy of biological solutions like yeast suspension. Multiple studies indicate that yeast membrane activities are the primary source of the harmonics detected from yeast suspensions. However, other investigations have demonstrated that at least part of the harmonics are mainly due to the nonlinear impedance of the electrode−electrolyte interface. Here, we want to find a mathematical model to analyze the odd harmonics generated by the interface. The KCl solution is the basis of the essential medium used in yeast culture. No study models the nonlinear behavior of the KCl solution using four-electrode. We used a sinusoidal voltage with a low frequency and a high amplitude to test an ionic solution with 1, 30, and 130 mM of potassium chloride. The voltage had no direct current component. Here, we show that the relationship between the amplitude of the excitation voltage and the third harmonic of the current can be accurately represented using the Butler−Volmer equation. Additionally, we analyze the impact of potassium chloride (KCl) concentration on the behavior of the third harmonic. This paper presents a method and model that can be used for nonlinear spectroscopy studies of biological solutions using a four-electrode system with the aim of deriving information from cells without considering the interface effect.

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