The derivatization and analysis of anticancer pharmaceuticals in the presence of tricyclic antidepressants by gas chromatography

Abstract

Summary. The evaluation of several derivatization procedures for the gas chromatographic analysis of selected anticancer pharmaceuticals (cyclophosphamide, iphosphamide, flutamide, chlorambucil, and melphalan) in the presence of tricyclic antidepressants was carried out. Among the methods, concerning trimethylsilylation, tertbutyldimethylsilylation, and methylation, the most useful was methylation using (trimethylsilyl)diazomethane (TMSD), which is a safe alternative for the common reaction with diazomethane. Most of the anticancer drugs were unstable during derivatization using silylating agents, while antidepressants were stable in all tested conditions. Melphalan was the only compound, for which the results of all tested procedures were not satisfying. The TMSD-based procedure was validated, giving the results possibly suitable for the screening purposes in contaminated environmental samples.

Simple Sonochemical Synthesis of Cupric Oxide Sphere Decorated Reduced Graphene Oxide Composite for the Electrochemical Detection of Flutamide Drug in Biological Samples

“…Currently, analytical techniques such as spectrophotometry, 6 polarography, 7 high performance liquid chromatography (HPLC), 8 flow-injection analysis, 9 stripping voltammetry 10 and gas-liquid chromatography (GLC) have been developed for the detection of flutamide. 11 However, most of these aforementioned techniques involve time-consuming procedures, tedious protocols, and high-cost operational techniques, and they require highly trained technicians. In comparison to such methods, the electrochemical detection is simple, highly selective and sensitive.…”