The detection of alloantibodies to subpopulations of human lymphocytes: An adaptation of the indirect anti-immunoglobulin rosetting reaction (IARR)

Bright, Susan; Munro, Alan; Lawson, Yvonne A.; Coombs, R.R.A.

doi:10.1016/0022-1759(78)90098-4

Cited by 9 publications

(1 citation statement)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A drop of fluorescein isothiocyanate solution in a pH 9 . 5 carbonate buffer (Bright et al, 1978;Nalet & Fournier, 1984) was placed on the cover-slip at room temperature in the dark for 1 h, after which it was rinsed off with water. This process yielded a fluorescent, denatured protein layer, with a thickness of the order of 1 m, which adhered firmly to the cover-slip.…”

Section: Microscopymentioning

confidence: 99%

A rapid‐flow perfusion chamber for high‐resolution microscopy

Kaplan

Bungay²,

Sullivan³

et al. 1996

Journal of Microscopy

View full text Add to dashboard Cite

Perfusion chambers employing laminar flow have dead volumes and unstirred layers which limit the minimum time required to effect a change in the local chemical environment of the sample. We have fabricated and tested a chamber capable of developing turbulent flow at reasonable flow rates of aqueous solutions. Transition to turbulence occurred at approximately equal to 1 mLs-1. To minimize dead space, a dual-exit cross-flow pattern was employed. The chamber was designed to mount on optical microscope stages for visual sample observation supplemented by a variety of techniques, such as fluorescence, light scattering and electrochemical monitoring. As indicated by fluorescence from a fluorescein-labelled protein film adherent to the chamber wall, local pH changes were produced within 200 ms. Use of the chamber is illustrated by measurements of stopped-flow kinetics in both calcium-triggered cortical granule exocytosis and influenza virus haemagglutinin-mediated cell-cell fusion.

show abstract

Section: Microscopymentioning

confidence: 99%