The detection of peste des petits ruminants (PPR) virus antigen by agar gel precipitation test and counter-immunoelectrophoresis

U., T.; Patrick, D. W.

doi:10.1017/s0022172400065165

Cited by 29 publications

(20 citation statements)

References 8 publications

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“…Serological tests such as agar gel immuno-diffusion test (AGID)/AGPT [101], counterimmuno-electrophoresis (CIE) [101] and indirect ELISA, although popular in earlier days for tentative diagnosis, do not differentiate PPR and RP infections [98,104]. Advent of cell culture and molecular biological techniques has allowed development of specific, rapid and sensitive diagnostics.…”

Section: Diagnosismentioning

confidence: 99%

Diagnosis and control of peste des petits ruminants: a comprehensive review

Balamurugan¹,

Hemadri²,

Gajendragad³

et al. 2013

VirusDis.

135

118

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Peste des petits ruminants (PPR) is an acute, highly contagious, world organization for animal health (OIE) notifiable and economically important transboundary viral disease of sheep and goats associated with high morbidity and mortality and caused by PPR virus. PPR is considered as one of the main constraints in augmenting the productivity of small ruminants in developing countries and particularly severely affects poor farmer's economy. The disease is clinically manifested by pyrexia, oculo-nasal discharges, necrotizing and erosive stomatitis, gastroenteritis, diarrhoea and bronchopneumonia. The disease can be diagnosed from its clinical signs, pathological lesions, and specific detection of virus antigen/antibodies/genome in the clinical samples by various serological tests and molecular assays. PPR is the one of the priority animal diseases whose control is considered important for poverty alleviation in enzootic countries. Availability of effective and safe live attenuated cell culture PPR vaccines and diagnostics have boosted the recently launched centrally sponsored control programme in India and also in other countries. This review article primarily focus on the current scenario of PPR diagnosis and its control programme with advancement of research areas that have taken place in the recent years with future perspectives.

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Section: Diagnosismentioning

confidence: 99%

Diagnosis and control of peste des petits ruminants: a comprehensive review

Balamurugan¹,

Hemadri²,

Gajendragad³

et al. 2013

VirusDis.

135

118

View full text Add to dashboard Cite

show abstract

“…A 1% (w/v) agar was prepared by boiling 1 g of agarose in 100 ml of barbitone acetate buffer. The CIEP protocol was essentially as described previously by Elhag Ali and Lees (1979), Majiyagbe et al (1984) and Obi and Patrick (1984).…”

Section: Counter Immnuo-electrophoresis (Ciep)mentioning

confidence: 99%

Antibody seroprevalences against Peste des Petits Ruminants (PPR) virus in sheep and goats in Sudan

Osman

Ali

Rahman³

et al. 2009

Trop Anim Health Prod

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Counter immnuo-electrophoresis (CIEP) andCompetitive ELISA (C-ELISA) tests were employed for seroprevalence of Peste des Petits Ruminants (PPR) infection in Sudan. The result of both tests showed high prevalence of PPRV antibodies in sheep and goats sera collected from six different regions of Sudan. Of the 519 serum samples examined for the presence of PPRV antibodies 307(59.15%) were positive by CIEP while 263(50.67%) were positive by C-ELISA. CIEP technique was shown to be more sensitive than C-ELISA technique for detection of PPRV antibodies (Kappa statistics 0.259). C-ELISA allowed rapid, simple, specific, sensitive and differential serodiagnosis of PPRV and RPV in sheep, goats and cattle. CIEP is, unlike competitive ELISA, is group-specific test and can not differentiate between PPR and RP infections. Despite its low specificity CIEP can be a useful indicative screening test for PPRV antibodies in flocks that neither been vaccinated nor otherwise exposed to PPR or RP virus. Results obtained suggest that CIEP, like the HI test, could be a useful screening test where it is not possible to use C-ELISA.

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“…Conventional techniques used for PPRV detection are: agar gel immunodiffusion (AGID) (Munir et al, 2009b), counter immunoelectrophoresis (CIEP) Obi & Ojeh, 1989), dot enzyme immunoassay (Perl et al, 1995;Obi & Patrick, 1984), differential immunohistochemical staining of tissue sections (Saliki et al, 1994), haemagglutination (HA) and haemagglutination inhibition (HI) tests (Raj et al, 2008;Saravanan et al, 2006;Manoharan, 2005), virus isolation (Manoharan, 2005;Brindha et al, 2001), competitive enzyme-linked immunosorbent assay (c-ELISA) (Ezeibe et al, 2008;Anderson et al, 1991), novel sandwich ELISA (Munir et al, 2009b;Anderson & McKay, 1994), immuno-capture enzyme-linked immunosorbent assay (IC-ELISA) (Abubakar et al, 2008;Saravanan et al, 2008;Khan et al, 2007;Singh et al, 2004;Libeau et al, 1994), immunofiltration (Diop et al, 2005), and latex agglutination tests (Keerti et al, 2009).…”

Section: Laboratory Diagnosis Of Pprmentioning

confidence: 99%