The detection of pro-inflammatory cytokines in exudates from dental pulp tissues

Wu, Qiyan; Li, Shanshan; Li, Ran; Chen, Xuejiao; Guo, Likun; Zheng, Ying

doi:10.1016/j.cyto.2022.155846

Cited by 9 publications

(1 citation statement)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, the inflammatory milieu in the periodontal region hinders stem cell-based alveolar bone regeneration. Levels of various pro-inflammatory cytokines including TNF-α are upregulated in the pulpitis-affected pulp tissue and periodontitis-affected alveolar bone [51,52]. TNF-α is frequently used in vitro cell cultures as an inflammatory cytokine mimicking the inflammatory situation in vivo [53].…”

Section: Discussionmentioning

confidence: 99%

Cannabidiol Rescues TNF-α-Inhibited Proliferation, Migration, and Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells

Zeng

Zhang

et al. 2023

Biomolecules

View full text Add to dashboard Cite

Strategies to promote dental pulp stem cells (DPSCs) functions including proliferation, migration, pro-angiogenic effects, and odontogenic/osteogenic differentiation are in urgent need to restore pulpitis-damaged dentin/pulp regeneration and DPSCs-based bone tissue engineering applications. Cannabidiol (CBD), an active component of Cannabis sativa has shown anti-inflammation, chemotactic, anti-microbial, and tissue regenerative potentials. Based on these facts, this study aimed to analyze the effect of CBD on DPSCs proliferation, migration, and osteogenic/odontogenic differentiation in basal and inflammatory conditions. Highly pure DPSCs with characteristics of mesenchymal stem cells (MSCs) were successfully isolated, as indicated by the results of flowcytometry and multi-lineage (osteogenic, adipogenic, and chondrogenic) differentiation potentials. Among the concentration tested (0.1–12.5 µM), CBD (2.5 μM) showed the highest anabolic effect on the proliferation and osteogenic/odontogenic differentiation of DPSCs. Pro-angiogenic growth factor VEGF mRNA expression was robustly higher in CBD-treated DPSCs. CBD also prompted the migration of DPSCs and CBD receptor CB1 and CB2 expression in DPSCs. TNF-α inhibited the viability, migration, and osteogenic/odontogenic differentiation of DPSCs and CBD reversed these effects. CBD alleviated the TNF-α-upregulated expression of pro-inflammatory cytokines TNF-α, interleukin (IL)-1β, and IL-6 in DPSCs. In conclusion, our results indicate the possible application of CBD on DPSCs-based dentin/pulp and bone regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%