There is a considerable and growing interest in the freezing and frozen storage of prepared food products. There is also a growing interest in the development of soybean products and their use in foods. I n view of these trends, and because of the scarcity of published data dealing with the oxidation of fat in food mixtures at low temperatures, particularly in the presence of food material of a possible antioxidant nature, the present study was undertaken.The antioxidant effect of soybean flour on purified substrates has been demonstrated. Hence this flour is a food material which might be expected to have antioxidant properties in a food mixture stored at low temperatures. Musher (1935) added raw soybean flour and bleached, extracted soybean flour to lard and found, using the Swift stability test, that both flours were effective antioxidants for lard. The raw flour was more effective than that which was bleached and extracted. Sylvester, Lampitt, and Ainsworth (1942) have presented data on the antioxidant effect on premier jus of soybean flour. They found that this flour had a stabilizing effect which was greater for fats having a long rather than a short initial induction period. They found also that the increase in the induction period was not proportional to the concentration of soybean flour, since a relatively smaller effect was obtained as the concentration of the flour was increased.The constituents of soybean flour which behave as fat antioxidants are probably cephalin, the tocopherols, and other substances. The stabilizing effect of commercial soybean lecithin is probably due to its content of cephalin, Olcott and Mattill (1946). Both tocopherol and soybean lecithin have been shown to act as antioxidants for lard, but when uaed together, a considerably increased effect has been observed. The cephalin present in soybean lecithin acts as a synergist with tocopherol, Libby (1945) andMattill (1945).Antioxidants other than tocopherols and cephalin have been demonstrated in soybean flour. Thus, Dahle and Nelson (1941) found that, an alcohol extract and phospholipid fraction had antioxidant properties for butter. Hilditch and Paul (1939) presented evidence for yet other antioxidant substances. Some of these, according to Golumbic (1942Golumbic ( , 1943, are similar to ~hromane-5~6-quinones.