The Determination of Cholinesterase in the Brain Tissue of Three Species of Fresh Water Fish and Its Inactivation in Vivo

Weiss, Charles M.

doi:10.2307/1931863

Cited by 76 publications

(20 citation statements)

References 13 publications

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“…In view of the observed drops of AChE activity, it should be considered whether it would be possible to reactivate AChE after fish transfer to clean water. In cas� of the insecticides under study it can be expected that after the exposition, further decrease of AChE may occur, as described by Weiss (1958Weiss ( , 1961. This statement is supported by observations of enzyme activity, undertaken during.…”

Section: Methodsmentioning

confidence: 55%

“…Blood was taken from heart with a syringe (Klontz and Smith 1968). Activity of AChE was estimated with Hestrin (1949) method, as modified by Weiss (1958), of peroxidasewith guaiacol method, according to Bach and Zubkova (Metelev 1971), and of catalasewith manganese metric method. Standard procedures were used for hematological studies (Klontz andSmith 1968, Blaxhall andDaisley 1973).…”

Section: Methodsmentioning

confidence: 99%

“…Attention was especially paid to the inhibition of cholinesterase activity in brain and other fish tissues, i.e. to the essential mechanisms of the effect of these compounds on fish organism (Weiss 1958, 1959, 1961, Williams and Sova 1966, Holland et al 1967, Gibson et al 1969, Coppage and Matthews 1974, Cook et al 1976, Metelev and Trostina 1969, Metelev 1972, Sakaguhi 1972. Hematological studies constitute successive step in the assessment of physiopathological effects.…”

Section: Introductionmentioning

confidence: 99%

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Effect of lethal concentrations of selected phosphoro-organic insecticides upon rainbow trout Salmo gairdneri Rich

Piotrowska¹

1980

Acta Ichthyol. Piscat.

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Tests of acute toxicity for rainbow trout fry of chlorfenviphos and bromfenviphos were undertaken in order to establish the Lc 50 values. Studies were also made on the effect of lethal concentrations, corresponding to 24 h LC 50 , upon the activity of selected enzymes and hematological indices. Activity of brain acethylcholinesterase, and blood peroxidase and catalase, was assessed. Hematological studies embraced: content of hemoglobin, hematocrit, number of red and white corpuscles, percentage share of lymphocytes, red/white cell ratio, and MCV, MCH, and MCHC indices.

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Section: Methodsmentioning

confidence: 55%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of lethal concentrations of selected phosphoro-organic insecticides upon rainbow trout Salmo gairdneri Rich

Piotrowska¹

1980

Acta Ichthyol. Piscat.

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“…The acetylcholinesterase (AChE) activity was determined using Weiss (1958) modifi cation of Hestrin's (1949) procedure; the blood peroxidase activity was detennined using Bach and Zubkova's guaiacol technique (Metelev, 1971), while the manganometric technique was used to detennine the blood catalase activity. Routine techniques were employed for the haematologic analyses (Klontz and Smith, 1968;Blaxhall and Daisley 1973).…”

Section: Methodsmentioning

confidence: 99%

“…The brain AChE activity recovery in many fish species undergoing a short-tenn exposure to a large number of organophosphate pesticides was comprehensively studied by Weiss (1958Weiss ( , 1959Weiss ( , 1961. He found the degree of inhibition to be a function of insecticide concentration, time of exposure, pesticide properties, and fish species.…”

mentioning

confidence: 99%

Effect of selected organophosphate insecticides in sublethal concentrations on rainbow trout Salmo gairdneri Rich

Piotrowska¹

1981

Acta Ichthyol. Piscat.

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Comparative studies on effects of CVP and BVP insectici des in sublethal concentrations on young rainbow trout (Salmo gairdneri Rich.) were carried out under laboratory conditions. The insecticide effect was inferred from enzy matic and haematologic analyses. One experiment involved a 14-day exposure to insecticide concentrations corresponding to 5% of 96-h LC 50 , followed by the brain acetylcholines terase, blood peroxidase and catalase activity assessment and determination of haematologic indices. The other experiment involved observing changes in the brain acetylcholinesterase activity over 30 days following the 24-h exposure to concentrations corresponding to 50% of 24-h LC so,

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The relationship between total cholinesterase activity and mortality in four butterfly species

Bargar

2012

Environmental Toxicology and Chemistry

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The relationship between total cholinesterase activity (TChE) and mortality in four butterfly species (great southern white [Ascia monuste], common buckeye [Junonia coenia], painted lady [Vanessa cardui], and julia butterflies [Dryas julia]) was investigated. Acute contact toxicity studies were conducted to evaluate the response (median lethal dose [LD50] and TChE) of the four species following exposure to the organophosphate insecticide naled. The LD50 for these butterflies ranged from 2.3 to 7.6 µg/g. The average level of TChE inhibition associated with significant mortality ranged from 26 to 67%, depending on the species. The lower bounds of normal TChE activity (2 standard deviations less than the average TChE for reference butterflies) ranged from 8.4 to 12.3 µM/min/g. As a percentage of the average reference TChE activity for the respective species, the lower bounds were similar to the inhibition levels associated with significant mortality, indicating there was little difference between the dose resulting in significant TChE inhibition and that resulting in mortality.

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The Determination of Cholinesterase in the Brain Tissue of Three Species of Fresh Water Fish and Its Inactivation in Vivo

Cited by 76 publications

References 13 publications

Effect of lethal concentrations of selected phosphoro-organic insecticides upon rainbow trout Salmo gairdneri Rich

Effect of lethal concentrations of selected phosphoro-organic insecticides upon rainbow trout Salmo gairdneri Rich

Effect of selected organophosphate insecticides in sublethal concentrations on rainbow trout Salmo gairdneri Rich

The relationship between total cholinesterase activity and mortality in four butterfly species

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