The determination of iron in blood plasma or serum

Abstract

Some time ago it became necessary in this department to have a rapid and reliable method for the determination of iron in blood serum or plasma. The method was required for teaching purposes on

Hepatoprotective properties of Morinda pubescens J.E. Smith (Morinda tinctoria Roxb.) fruit extract

“…The iron content in the liver homogenates was determined by the method of Ramsay (1957). LPO was assessed by the thiobarbituric acid-reactive substance (TBARS) method (Ohkawa et al, 1979).…”

Hepatoprotective effect of allicin on tissue defense system in galactosamine/endotoxin challenged rats

“…The liver was excised immediately and homogenized in ice-cold 0.1 M Tris-HCl buffer in a Potter Elvehjem. The homogenate was used for the estimation of iron by the method of Ramsay (1957), ferritin by the method of Drysdale and Munro (1966), lipid peroxides (LPO) by the method of Ohkawa et al (1979), superoxide dismutase (SOD) by the method of Misra and Fridovich (1972), catalase (CAT) by the method of Takahara et al (1960), reduced glutathione (GSH) by the method of Sedlak and Lindsay (1968), glutathione-S-transferase (GST) by the method of Habig et al (1974) and glutathione peroxidase (GPX) by the method of Rotruck et al (1973).…”

Unusual Haemoglobin Variant in a Gujerati Indian

“…These findings accord with the lowered cell volume (66.8 cubic microns) and the increased resistance to hypotonic saline, and are suggestive of thalassaemia minor. The plasma iron of this girl was high (method of Ramsay, 1953), which is in favour of the same diagnosis.…”