The Determination of Listeria monocytogenes in Foods with Optical Biosensors

Abstract

Listeria monocytogenes is a food-borne intracellular pathogen that is resistant to adverse conditions of food processing and is a causative agent of high mortality listeriosis disease. Analysis of large food lots is limited by culture methods. Nowadays, although the conventional culture methods have been adopted as the gold standard method, they can be insufficient to control food parties that reach very large quantities. Biosensors with on-site detection potential and their characterizing with high accuracy and precision offers many opportunities for real-time estimation of industrial contamination. As the most promising technique for rapid detection of L.monocytogenes, ones based on surface plasmon resonance (SPR) and fiber optic biosensors (FOBs) where direct or indirect detections can be made by detecting the interaction of the biomarker element with the optical field without marking or labeling, have come to the fore. SPR and FOBs can be detected directly in the range of 10 2-10 6 cfu/ml and ≤10 1-10 3 cfu/ml, respectively.

Listeria monocytogenes in foods—From culture identification to whole‐genome characteristics

“…The electronic or optical signal generated by biosensors are measured and recorded in proportion to the specific biological interaction between the analyte and the recognition molecule (Turner, 2000 ). Using biosensors, several different targets can be detected, from small molecular weight proteins to bacterial cells (Bìberoğlu, 2020 ).…”

Sensitivity-Tunable Terahertz Liquid/Gas Biosensor Based on Surface Plasmon Resonance with Dirac Semimetal

“…However, compared with traditional optical biosensor schemes, the SPR biosensor has many obvious and unique advantages, such as high sensitivity, real-time detection, easy penetration and no loss [ 20 , 21 ]. Therefore, SPR sensing technology has been widely used in biochemical analysis [ 22 ], food safety [ 23 ], medical diagnosis [ 24 ] and other fields with remarkable effects, such as the detection of staphylococcal enterotoxin in milk [ 25 ], drug residue detection [ 26 , 27 ], real-time disease diagnosis [ 28 ], gas detection [ 29 ], etc. However, the traditional SPR structure usually uses Otto structure or Kretschmann–Raether (KR) structure based on noble metals (such as Au [ 30 ], Ag [ 31 ], Al [ 32 ], etc.)…”