The Determination of Plasma Uric Acid

Bulger, Harold A.; Johns, H.

doi:10.1016/s0021-9258(18)51332-6

Cited by 50 publications

(6 citation statements)

References 7 publications

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“…The second group served as treated group and was given frankincense in the drinking water during the whole duration of the experiment. After 15 and 30 days of treatment, the rats were anesthetized with ether, and blood was collected from the livers and kidneys; some biochemical analyses were performed such as aspartate aminotransferase (AST) (Saris, 1978), alanine aminotransferase (ALT) (Bergmeyer et al, 1978) and alkaline phosphatase (ALP) (Williams, 1984), bilirubin (Jendrassik and Grof, 1938), urea (Talke and Schubert, 1965), uric acid (Bulgar and Johns, 1941), and creatinine (Larsen, 1972), which were measured by Dimension (DAD BEHRING Company, USA).…”

Section: Methodsmentioning

confidence: 99%

Identifying frankincense impact by biochemical analysis and histological examination on rats

Yousef¹

2011

Saudi Journal of Biological Sciences

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Frankincense (Gum Olibanum), made from resins of Burseraceae family, grows in Somalia, India and Yemen. Many years ago the oldest doctors used this plant for treatment of many diseases. This study identifies frankincense impact by biochemical analysis and histological examination on rats. In this study, forty male Wister Albino rats weighing 70-100 g were maintained in clean cages. The rats were divided into 2 groups, each group contained 20 rats. Frankincense extract was prepared by heating distilled water (400 ml) to 80 °C and soaking 20 g of herbs for about 60 min. After cooking at room temperature the dose was given orally through special drinking bottles daily. The first group acted as control drinking water. The second group served as treated group and was given frankincense in the drinking water during the whole duration of the experiment. After 15 and 30 days of treatment, the rats were anesthetized with ether, and blood was collected from the livers and kidneys; some biochemical analyses were performed including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and non-bilirubin, urea, uric acid, and creatinine. Rats were killed by cervical decapitation of livers and kidneys. Each group was divided into 2 parts. The first part was used for the determination of glutathione (GSH), glucose 6 phosphate dehydrogenase (G6PDH), xanthine oxidase (XO), malonyldealdehyde (MDA), nitric oxide (NO), and xanthine oxidase (XO). The second part of livers and kidneys was kept in formalin solution (10%) and stained by Hematoxylin and Eosin (H & E), to be used for histological examination. I demonstrated in the biochemical analysis in the serum, tissue and histological examination, different impact between group (B) and group (A), and that frankincense is not absolutely safe and that precautions must be taken during it's us as a traditional medicine and that increase the awareness with safety and health hazards of many other traditional medicine is critically needed.

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Section: Methodsmentioning

confidence: 99%

Identifying frankincense impact by biochemical analysis and histological examination on rats

Yousef¹

2011

Saudi Journal of Biological Sciences

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“…The use of uricase enzyme (UOX) is considered to be a selective approach for UA detection and it was introduced by Bulger and Johns in 1941. 55 Reports on different methods/techniques for determination of UA suffer from drawbacks such as, time-consuming and laborious, complex to perform and expensive. Meanwhile, the enzymatic test kits are sensitive, simple and specific to detect UA which are made of uricase and peroxidase.…”

Section: Enzymatic and Non-enzymatic Biosensorsmentioning

confidence: 99%

Review—Electrochemical Sensing of Uric Acid: Methods and Recent Materials

Dhinasekaran,

John,

Subashchandran

2024

J. Electrochem. Soc.

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Uric acid (UA) is an important biomarker in blood to diagnosis diseases linked with hyperuricemia. Although several detection methods exist for UA sensing, electrochemical method has emerged as a promising alternative. For effective performance of a biosensor, the choice of electroactive material plays a crucial role. The developed electrodes are enzymatic and non-enzymatic with modified nano-structures of metal oxides, ferrites and carbon-based materials. Several combinations of nanocomposites using metal oxides with carbon-based compounds show promising results for biosensor applications. This is attributed to its functional groups, higher surface area and porous nature that can improve the sensing performance as it requires only quick-time processing with inexpensive and direct detection methods. The electrochemical method uses anodic peak current which is the analytical signal to sense the electrochemical oxidation of UA. This technique paves a new way to make electrodes for point-of-detection devices in near future. It could be the next generation of non-invasive analysis for food hygiene as well as biomedical and clinical applications. This review focuses on materials used in electrochemical sensing of UA and discusses on the application of different electrochemical techniques in UA detection.

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“…16 Lipid profile was estimated by automatic analyzers using conventional methods on Hitachi 902 auto-analyzer (Japan). 17 Plasma uric acid was measured by modified uricase method, 18 creatinine was estimated by modified kinetic Jaffe's method immediately on Hitachi 902 autoanalyzer (Japan). Testing for proteinuria was performed with multistix urine reagent strips (Siemens) from PE patients.…”

Section: Measurementsmentioning

confidence: 99%

Examination of hepato-renal functions and lipid panel among pregnant women with preeclampsia in Sétif, Algeria

Bourouba¹,

Benantar²,

Ayati³

et al. 2020

J Biol Res

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Preeclampsia (PE) is a multisystem pregnancy disease inflecting maternal and fetal mortality and morbidity. This case-control study aims to determine the biochemical changes in lipid profile, hepatic and renal functions among sixty late-onset preeclamptic women compared to sixty age-matching normotensive pregnant women from the Maternity Hospital in Sétif, Algeria. Several biomarkers were collected and have been determined by appropriate kits. Association between various parameters was evaluated using Pearson’s correlation coefficient, with significance at p<0.05. The PE women showed a significant increase in serum aminotransferase, gamma-glutamyl-transferase, alkaline phosphatase, as well as total cholesterol, triglycerides, urea, uric acid, and creatinine levels. However, a significant decrease in platelets, total protein, albumin, and plasma sodium levels was found in PE group. A positive correlation between aminotransferases and renal biomarkers was observed. PE has deleterious effects on liver and renal functions, and complication’s progression could be predicted by evaluating hepatic and renal biomarkers as well as lipid profile. Further studies on maternal and neonatal outcomes in cases of PE are needed for a better understanding of this disease’s pathophysiology in Algeria.

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The Determination of Plasma Uric Acid

Cited by 50 publications

References 7 publications

Identifying frankincense impact by biochemical analysis and histological examination on rats

Identifying frankincense impact by biochemical analysis and histological examination on rats

Review—Electrochemical Sensing of Uric Acid: Methods and Recent Materials

Examination of hepato-renal functions and lipid panel among pregnant women with preeclampsia in Sétif, Algeria

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