The determination of thiocyanate in blood serum

Bowler, Rick

doi:10.1042/bj0380385

Cited by 175 publications

(50 citation statements)

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“…Dried blood spots in ®lter paper were analysed for whole blood TSH and serum T 4 using immunossay (Torresani & Scherz, 1986). Urinary thiocyanate (SCN) was analyzed by a colorimetric method (Bowler, 1944). Normal reference values are: UI, 50 ± 250 mgal; UIaSCN, b 3 mgamg (DeLange et al, 1983); serum Se, 65 ± 105 mgal; serum retinol, 0.35 ± 1.75 mmolal; whole blood TSH,`3.5 mUal; serum T 4 , 65 ± 165 nmolal.…”

Section: Methodsmentioning

confidence: 99%

Effect of oral iodized oil on thyroid size and thyroid hormone metabolism in children with concurrent selenium and iodine deficiency

Zimmermann

Adou²,

Torresani³

et al. 2000

Eur J Clin Nutr

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Objectives: To determine the ef®cacy of oral iodized oil in goitrous children who are both selenium (Se) and iodine de®cient; to investigate if Se status modi®es the response of iodine de®cient, goitrous children to oral supplementation with iodized oil. Design: A longitudinal intervention trial. Setting: Two rural villages in the western Co Ãte d'Ivoire. Subjects: 51 goitrous non-anemic schoolchildren with both iodine and Se de®ciency. Intervention: Each child received an oral dose of 0.4 ml iodized poppyseed oil containing 200 mg of iodine. They were followed for 1 y with measurements of urinary iodine (UI), thyrotropin (TSH), thyroxine (T4), and thyroid volume by ultrasound. Results: At baseline all children were goitrous and Se de®cient; median UI was 29 mgal and mean serum Se (s.d.) was 14.8 (10.7) mgal. After receiving iodized oil, thyroid volume decreased signi®cantly vs baseline at 10, 15, 30 and 50 weeks (P`0.001). At 50 weeks mean percentage change in thyroid volume from baseline was746.6% and only ®ve children remained goitrous. Median TSH values at 5, 10, 15, 30 and 50 weeks were reduced signi®cantly (P`0.001) compared to baseline. Among individual children the severity of Se de®ciency predicted the degree of response to iodized oil. Baseline serum Se and percentage change in thyroid volume from baseline at 50 weeks were strongly correlated (r 2 0.554). Baseline Se and percentage decrease in TSH from baseline at 30 weeks were also well-correlated (r 2 0.467). Conclusion: Although more severe Se de®ciency partially blunts the thyroid response to iodine supplementation, oral iodized oil is an effective method for iodine repletion in goitrous children who are Se de®cient.

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Section: Methodsmentioning

confidence: 99%

Effect of oral iodized oil on thyroid size and thyroid hormone metabolism in children with concurrent selenium and iodine deficiency

Zimmermann

Adou²,

Torresani³

et al. 2000

Eur J Clin Nutr

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“…Salivary thiocyanates were measured by spectrophotometry after addition of ferric chloride (Sigma) according to the method described by Bowler (23).…”

Section: Populationmentioning

confidence: 99%

Does In Utero Exposure to Heavy Maternal Smoking Induce Nicotine Withdrawal Symptoms in Neonates?

et al. 2004

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“…Concentration of VLDL-C and LDL-C was calculated as the difference between serum cholesterol and HDL-C. To assess smoking status, serum levels of thiocyanate were measured by a modification of the Bowler method. 9 A smoker was defined as one who had a serum thiocyanate level ^90 fiMJl. The coefficient of variation between blind duplicates for serum thiocyanate was 5.6%.…”

Section: Serum Measurementsmentioning

confidence: 99%

Apo B insertion/deletion polymorphisms are associated with atherosclerosis in young black but not young white males. Pathobiological Determinants of Atherosclerosis in Youth (PDAY) Research Group.

Hixson

McMahan

McGill

et al. 1992

Arterioscler Thromb

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Strong, and the Pathobiological Determinants of Atherosclerosis in Youth (PDAY) Research Group* Investigators in eight communities collected aortas, right coronary arteries, blood and liver samples, and associated information from 872 young males, aged 15-34 years, who died of external causes. Pathologists graded the arteries for atherosclerotic lesions, and a central laboratory measured lipoprotein cholesterol concentrations. Apollpoprotein (apo) B sequences were amplified in hepatic DNA samples to determine genotypes for length polymorphisms in the signal peptide of apo B. In addition to the insertion (ins) allele (27-amino acid signal peptide) and the deletion (del) allele (24 amino acids), we detected a rare allele (ins*) in whites with an in-frame insertion of two Leu codons In a region that normally contains six Leu codons. The frequency for the apo B del allele was lower in blacks than in whites (p<0.0001). In blacks, homozygotes for the ins allele had the lowest levels of serum cholesterol and very low plus low density lipoprotein cholesterol (VLDL+LDL-C), homozygotes for the del allele had the highest levels, and heterozygotes had intermediate levels (/?=0.0509 for cholesterol, p=0.0530 for VLDL+LDL-C), but no differences were found in whites. In blacks, homozygotes for the ins allele had the least involvement of the thoracic and the abdominal aorta with lesions, homozygotes for the del allele had the greatest involvement, and heterozygotes had intermediate involvement (p=0.0328 for thoracic aorta, p=0.0104 for abdominal aorta), but no differences were found in whites. In blacks, apo B ins/dei genotype accounted for 1.2% of the observed variation in lesions of the thoracic aorta and 1.7% of the variation in those of the abdominal aorta. The association of apo B ins/del genotypes with lesions in young blacks but not hi young whites may contribute to explaining the excess of fatty streaks previously observed in young blacks. ( A therosclerosis is a lifelong process that begins / \ early in life and results in clinically manifest •i-J L coronary heart disease in middle age and later. Risk factors for coronary heart disease in adults (age, smoking, and serum lipoprotein cholesterol levels) are associated with the extent and severity of atherosclerosis in young males 15-34 years of age.1 Common genetic variants of human apolipoprotein (apo) E also are associated with differences in lipid risk factors and atherosclerosis in young males.2 In this study, we report the association of common length polymorphisms in the signal peptide of human apo B with atherosclerotic lesions in young people. The common alleles are the insertion (ins) allele, which contains a 27-amino acid signal peptide, and the deletion (del) allele, with a From the Pathobiological Determinants of Atherosclerosis in Youth (PDAY) Research Group ('individual members and affiliations are listed in the "Appendix").Supported by grant HL-33913 from the National Heart, Lung, and Blood Institute, Bethesda, Md., and by other grants supporting PDAY inve...

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The determination of thiocyanate in blood serum

Cited by 175 publications

References 0 publications

Effect of oral iodized oil on thyroid size and thyroid hormone metabolism in children with concurrent selenium and iodine deficiency

Effect of oral iodized oil on thyroid size and thyroid hormone metabolism in children with concurrent selenium and iodine deficiency

Does In Utero Exposure to Heavy Maternal Smoking Induce Nicotine Withdrawal Symptoms in Neonates?

Apo B insertion/deletion polymorphisms are associated with atherosclerosis in young black but not young white males. Pathobiological Determinants of Atherosclerosis in Youth (PDAY) Research Group.

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