The Determination of Threonine by the Use of Periodate

Nicolet, Ben H.; Shinn, L. A.

doi:10.1016/s0021-9258(18)51413-7

Cited by 84 publications

(7 citation statements)

References 8 publications

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“…what accurate appraisal of serine and threonine. 2 An application of these methods to insulin has given the results shown. All figures given are corrected for moisture and for ash.…”

Section: Sirmentioning

confidence: 99%

A Determination of the Hydroxy Amino Acids of Insulin

Nicolet¹,

Shinn²

1941

J. Am. Chem. Soc.

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have demonstrated, in a series of experiments in which the relationship of the starting material to the product of bio-transformation is not subject to question, that bacterial reduction of A4-3-ketosteroids of the hormone series follows an entirely different course, in which the A4-double bond invariably is saturated prior to reduction of the C3-carbonyl group. The bioreduction of androstenedione and of testosterone was studied in a number of instances, but in no case was dehydroisoandrosterone or other A6-unsaturated steroid encountered as a reduction product.Of still greater significance to the question of the origin of the dehydroisoandrosterone found in urine, is the direct experiment of N. H. Callow [Biochem. J., 33, 559 (1939)]. Callow found that administration of testosterone propionate to a male patient resulted in an unmistakable increase in the urinary excretion of androsterone and 3 -hydroxyaetiocholanone-17, but that there was no evidence of the conversion of the administered A4-3-ketosteroid into dehydroisoandrosterone. Thus the present evidence, in our opinion, is contradictory to Marker's hypothesis.

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“…what accurate appraisal of serine and threonine. 2 An application of these methods to insulin has given the results shown. All figures given are corrected for moisture and for ash.…”

Section: Sirmentioning

confidence: 99%

A Determination of the Hydroxy Amino Acids of Insulin

Nicolet¹,

Shinn²

1941

J. Am. Chem. Soc.

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show abstract

“…The globulins were prepared by alkaline extraction followed by acid precipitation,' and by salt extraction followed by dilution. They contained approximately 18 per cent combined dicarboxylic acids (by amide nitrogen determination), 12 per cent arginine, 9 per cent leucine, 8 per cent phenylalanine, 6 per cent valine, 5 per cent lysine, 3 per cent each of histidine, methionine, tyrosine, serine, and threonine, 2 per cent isoleucine, and 1 per cent each of tryptophane and cystine.…”

Section: Preparation Of Protein Fractionsmentioning

confidence: 99%

“…Threonine and Serine. Hydrochloric acid hydrolyzates containing the equivalent of 300 to 450 mg. of protein were used for the determination of threonine and serine by the method described by Shinn and Nicolet (11, 18). Periodic acid was employed to oxidize the two quantitatively to acetaldehyde and formaldehyde, respectively.…”

Section: Determination Of Amino Acidsmentioning

confidence: 99%