The determination of transfer ribonucleic acid by aminoacylation

Rubin, Inger; Kelmers, A.D.; Goldstein, Gérald

doi:10.1016/0003-2697(67)90298-9

Cited by 70 publications

(20 citation statements)

References 13 publications

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“…Studies with microbial systems (Marshall & Zamecnik, 1969 Kosakowski, Neidhardt & Bock, 1970;Cole & Schimmel, 1970) have demonstrated good reaction rates in vitro. Similarly, the studies by Rubin, Kelmers & Goldstein (1967), Loftfield & Eigner (1968), Anderson (1969), Shearn & Horowitz (1969), Mehler (1970) and others, have clearly shown controlling effects of many factors on aminoacyl-tRNA synthesis.…”

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confidence: 56%

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Factors controlling aminoacyl-transfer-ribonucleic acid synthesis in vitro by a plant system

Tao¹,

Hall²

1971

Biochemical Journal

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confidence: 56%

“…The bean-leaf system differs from the Escherichia coli system in that K+ had a marked effect on the rate of reaction, whereas no effect was observed with the microbial system (Rubin et al 1967). Significant effects of tRNA and enzyme concentrations on aminoacyl-tRNA formation were also observed (Figs.…”

Section: Discussionmentioning

confidence: 99%

Factors controlling aminoacyl-transfer-ribonucleic acid synthesis in vitro by a plant system

Tao¹,

Hall²

1971

Biochemical Journal

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“…Studies with microbial systems (Marshall & Zamecnik, 1969; Kosakowski, Neidhardt & Bock, 1970;Cole & Schimmel, 1970) have demonstrated good reaction rates in vitro. Similarly, the studies by Rubin, Kelmers & Goldstein (1967), Loftfield & Eigner (1968), Anderson (1969), Shearn & Horowitz (1969), Mehler (1970) and others, have clearly shown controlling effects of many factors on aminoacyl-tRNA synthesis.…”

mentioning

confidence: 58%

Factors controlling aminoacyl-transfer-ribonucleic acid synthesis in vitro by a plant system

Tao

Hall

1971

Biochemical Journal

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1. Factors affecting aminoacyl-tRNA synthesis in vitro by cell-free preparations from bean leaves were investigated. 2. Evidence was obtained that optimum concentrations as well as correct ratios of Mg(2+) and ATP are required for aminoacyl-tRNA synthesis in the bean-leaf system. 3. The results indicated that pH is a controlling factor having differential effects on the formation of individual aminoacyl-tRNA species. The possible micro-regulatory function of pH in protein synthesis in vivo is discussed with special reference to alanyl-tRNA formation. 4. Very low rates of alanine-stimulated pyrophosphate exchange were observed in the absence of tRNA. This observation is discussed relative to proposals about the mechanism of aminoacyl-tRNA synthesis.

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“…Interestingly, cationic moieties have been shown to play an important role in polynucleotide biochemistry. Many cations stabilize tertiary polynucleotide structures Crothers, 1976a, 1976b); Mg 2+ and Mn 2+ are necessary for the amino acylation of tRNA (Gartland and Suekoka, 1966;Rubin et al, 1967), and histones, containing alkylammonium groups, are always found in conjunction with DNA.…”

Section: Introductionmentioning

confidence: 97%

Partitioning of amino acids and nucleotides between water and micellar hexadecyltrimethylammonium halides

1977

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Using quantitative gel filtration techniques partition coefficients, Kp-values, have been determined between aqueous cationic micellar hexadecyltrimethylammonium bromide, CTAB, and several biomonomer. Kp-values for 5'-adenylic acid, 5'-cytidylic acid, 5'-guanylic acid, 5'-uridylic acid and 5'-thymidylic acid are 1,400 +/- 150. Nucleotides bind to CTAB micelles effectively, but nonselectively. Conversely, the binding of tRNAs to micellar CTAB is selective. Kp-values for glutamic acid II, tyrosine and phenylalanine tRNAs (in 1.0MNaCl) are 520, 3,100 and 5,600, respectively. Kp-values for the binding of alanine, arginine, aspartic acid, glutamic acid, glycine, histidine, phenylalanine, serine, threonine and tryptophan to micellar CTAB are less than 8. Conversion of unitless Kp-values for the binding of amino acids, nucleotides and nucleosides to both anionic and cationic micelles, to K (in 1/g) values allows the comparison of clays and micelles as prebiotic concentrating media. Using correlations between surface densities of the biomonomers and their binding constants, it is shown that aqueous micelles (at pH = 8) are a better concentrating media than are clays.

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The determination of transfer ribonucleic acid by aminoacylation

Cited by 70 publications

References 13 publications

Factors controlling aminoacyl-transfer-ribonucleic acid synthesis in vitro by a plant system

Factors controlling aminoacyl-transfer-ribonucleic acid synthesis in vitro by a plant system

Factors controlling aminoacyl-transfer-ribonucleic acid synthesis in vitro by a plant system

Partitioning of amino acids and nucleotides between water and micellar hexadecyltrimethylammonium halides

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