The determination of vitamin D3 in bovine milk by liquid chromatography mass spectrometry

Trenerry, V. Craige; Plozza, Timothy Edward; Caridi, Domenico; Murphy, Stacey

doi:10.1016/j.foodchem.2010.09.097

Cited by 50 publications

(24 citation statements)

References 30 publications

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“…For physiological samples such as human plasma, vitamins A, E, 25(OH) D 2 and 25(OH) D 3 were separated on a RP-C 18 column with gradient elution and all compounds were eluted in less than 15 min (Alvarez & De Mazancourt, 2001). Vitamins A, E and β-carotene in bovine milk were simultaneously separated on RP-C 18 column using methanol:water solvent system in about 30 min (Plozza, Trenerry, & Caridi, 2012) but the separation of vitamin D 3 was not included and reported as a separate method (Trenerry, Plozza, Caridi, & Murphy, 2011). Another LC/MS method was described for the determination of vitamin A, vitamin A acetate, vitamin A palmitate, vitamin E (α-tocopherol), vitamin E acetate, and coenzyme Q10 in multivitamin dietary supplements using a RP-C 30 stationary phase.…”

Section: Introductionmentioning

confidence: 99%

Quantification of selected fat soluble vitamins and carotenoids in infant formula and dietary supplements using fast liquid chromatography coupled with tandem mass spectrometry

Nimalaratne

Sun

et al. 2014

Food Research International

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Section: Introductionmentioning

confidence: 99%

Quantification of selected fat soluble vitamins and carotenoids in infant formula and dietary supplements using fast liquid chromatography coupled with tandem mass spectrometry

Nimalaratne

Sun

et al. 2014

Food Research International

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“…Dane z lat 2009-2013 sugerują obecność witaminy D 3 w mleku krowim (niewzbogacanym! w witaminę D) na poziomie 0,8-16 UL/l [33][34][35], podczas gdy we wspomnianych latach ubiegłego wieku zawartość ta oceniana była śred-nio na 40 U/l [36].…”

Section: Przyczyny Niedoboru Witaminy Dunclassified

Vitamin D – recommendations or the need for individualization of dosage?

Kołłątaj¹,

Kołłątaj²,

Klatka³

2015

Pediatr. Endocrinol.

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Due to the general lack of vitamin D, in the countries of Central Europe, there are published recommendations suggesting the need for supplementation of cholecalciferol. Objective. The aim of this study was to evaluate the effectiveness of prevention of vitamin D deficiency in children and adolescents suffering from hypovitaminosis D in the absence of medical history suggesting the risk of hypovitaminosis and indications for determining the levels of 25OHD (guidelines on cholecalciferol supplementation published in 2013). Material. The study included 105 children and adolescents -patients of the Department of Paediatric Endocrinology and Diabetology as well as Endocrinology Clinic of the University Children's Hospital in Lublin. These patients had not taken suggested vitamin D 3 supplementations before. Methods. Patients with diagnosed hypovitaminosis D have been receiving oral doses of cholecalciferol -1000 IU/day for a period of not less than six weeks. Then their serum levels of 25OHD were checked again. All of them have been treated with vitamin D product under the trade name Vigantoletten tablets 1000 IU. Results. 47 patients (approx. 45%) did not have a sufficiently effective cholecalciferol substitution despite supplementation conducted in accordance with the recommendations, and only 42 people (40%) had serum 25OHD concentrations exceeding 35 ng/ml. Analysis of the body weight, body surface area and increments in serum 25OHD levels relationships do Słowa kluczowe witamina D, rekomendacje, hipowitaminoza, indywidualizacja, dawkowanie Streszczenie Z uwagi na powszechny niedobór witaminy D w krajach Europy Środkowej obowiązują rekomendacje sugerujące konieczność stosowania suplementacji cholekalcyferolu. Cel. Celem niniejszej pracy była ocena skuteczności profilaktyki niedoboru witaminy D stosowanej u dzieci młodzieży, u których rozpoznano hipowitaminozę D przy braku danych z wywiadu sugerujących ryzyko hipowitaminozy i wskazań do oznaczania poziomu 25OHD (wytyczne suplementacji z 2013 r.). Materiał. Badaniem objęto 105 dzieci i nastolatków -pacjentów Kliniki Endokrynologii i Diabetologii Dziecięcej oraz Poradni Endokrynologicznej Uniwersyteckiego Szpitala Dziecięcego im. A. Gębali w Lublinie. Osoby te uprzednio nie przyjmowały suplementacyjnych dawek witaminy D 3 . Metody. Po stwierdzeniu hipowitaminozy pacjenci otrzymywali doustne dawki cholekalcyferolu -1000 j.m./dobę przez okres nie krótszy niż 6 tygodni, a następnie powtórnie mieli oznaczane surowicze poziomy 25OHD. U wszystkich stosowano preparat handlowy witaminy -Vigantoletten tabl. a' 1000 j.m. Wyniki. 47 badanych (ok. 45%) nie miało wystarczająco skutecznej substytucji cholekalcyferolu pomimo suplementacji prowadzonej zgodnie z rekomendacjami, a tylko 42 osoby (40%) miały stężenia 25OHD przekraczające 35 ng/ ml. Analiza zależności: masa ciała i powierzchnia ciała a przyrosty surowiczego stężenia 25OHD wskazują na fakt, iż wpływ doustnej dawki witaminy D 3 na surowiczy poziom 25OHD jest osobniczo zmienny.

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“…Several techniques have been used for analyzing cholecalciferol such as combined thin layer and gas chromatography [4], high performance liquid chromatography [5][6][7][8][9], liquid chromatographymass spectrometry (LC-MS) [10][11][12], supercritical fluid chromatography [13], supercritical fluid chromatography-mass spectrometry (SFC-MS) [14], voltammetry [15,16] and bioassay [17]. Although, chromatographic methods offer the best approach to accurate determination of cholecalciferol, electrochemical techniques provide simple alternatives in this topic.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Behavior of Cholecalciferol on a Multiwalled Carbon Nanotube Modified Glassy Carbon Electrode

Kahya

Cittan

Çelik

2018

Cumhuriyet Science Journal

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Herein, electrochemical behavior of cholecalciferol on a multiwalled carbon nanotube modified glassy carbon electrode was evaluated by using cyclic voltammetry. Voltammetric determination of cholecalciferol on the prepared modified electrode was carried out using linear sweep voltammetry. A good linearity was obtained with a correlation coefficient of 0.9914 between 5×10 -5 -1×10 -3 M. The LOD and LOQ values were calculated as 1.7×10 -5 and 5.1×10 -5 M, respectively. The response of the proposed electrode was sufficiently repeatable for determining cholecalciferol. Finally, the proposed modified electrode was successfully applied to the determination of cholecalciferol in a commercial oral solution that contains 300.000 I.U cholecalciferol/mL. A simple liquid-liquid extraction technique by using methanol was followed to extract cholecalciferol from the oral liquid. The results obtained with the proposed method were in agreement with cholecalciferol content of the commercial oral solution.Özet. Bu çalışmada, kolekalsiferolün çok duvarlı karbon nanotüp modifiye camsı karbon elektrot üzerindeki elektrokimyasal davranışı, döngüsel voltammetri yöntemi kullanılarak değerlendirilmiştir. Hazırlanan modifiye elektrot yüzeyinde kolekalsiferolün voltammetrik tayini, doğrusal taramalı voltammetri yöntemi kullanılarak gerçekleştirilmiştir. 5×10 -5 -1x10 -3 M konsantrasyon aralığında 0.9914 korelasyon katsayısı ile iyi bir doğrusallık elde edilmiştir. LOD ve LOQ değerleri sırasıyla 1.7×10 -5 ve 5.1×10 -5 M olarak hesaplanmıştır. Önerilen elektrodun cevabı kolekalsiferolün belirlenmesi için yeterince tekrarlanabilirdir. Önerilen modifiye elektrot, 300.000 I.U kolekalsiferol/mL içeren ticari bir oral solüsyonda kolekalsiferol tayini için başarıyla uygulanmıştır. Oral solüsyondan kolekalsiferolü ekstrakte etmek için metanol kullanılarak gerçekleştirilen basit bir sıvı-sıvı ekstraksiyon tekniği izlenmiştir. Önerilen yöntem ile elde edilen sonuçlar, ticari oral solüsyonun kolekalsiferol içeriği ile uyum içerisindedir.Anahtar Kelimeler: Kolekalsiferol, Çok duvarlı karbon nanotüp, Voltammetri.

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The determination of vitamin D3 in bovine milk by liquid chromatography mass spectrometry

Cited by 50 publications

References 30 publications

Quantification of selected fat soluble vitamins and carotenoids in infant formula and dietary supplements using fast liquid chromatography coupled with tandem mass spectrometry

Quantification of selected fat soluble vitamins and carotenoids in infant formula and dietary supplements using fast liquid chromatography coupled with tandem mass spectrometry

Vitamin D – recommendations or the need for individualization of dosage?

Electrochemical Behavior of Cholecalciferol on a Multiwalled Carbon Nanotube Modified Glassy Carbon Electrode

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