The development of bioartificial nerve grafts for peripheral-nerve regeneration

Heath, Carole A.; Rutkowski, Gregory E.

doi:10.1016/s0167-7799(97)01165-7

Cited by 194 publications

(129 citation statements)

References 43 publications

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“…Common grafting in surgery was autograft or nerve removal from elsewhere of the body. Unfortunately, autografts had limitations such as body injury, repeated surgery and disproportion of grafted nerve tissue in terms of size and structure of nerve tissue (Evans, 2000;Heath & Rutkowski, 1998) also; transplantation of allograft or xenograft had similar problems such as stimulation of the immune system (Brunelli et al, 1993;Tong et al, 1994;Fansa et al, 2002;Barcelos et al, 2003;de Ruiter et al, 2009). The studies were conducted on the application of artifi cial neural tube to form neural cords (Archibald et al, 1995;Fields et al, 1989;Keeley et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

A nanofibrous PHBV tube with Schwann cell as artificial nerve graft contributing to Rat sciatic nerve regeneration across a 30-mm defect bridge

Biazar

Keshel

2013

Cell Communication & Adhesion

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Section: Introductionmentioning

confidence: 99%

A nanofibrous PHBV tube with Schwann cell as artificial nerve graft contributing to Rat sciatic nerve regeneration across a 30-mm defect bridge

Biazar

Keshel

2013

Cell Communication & Adhesion

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“…Various synthetic biomaterials have been studied in the construction of artificial nerve conduits. The ideal artificial nerve conduit should have certain biochemical and mechanical properties such as biocompatibility, ability to regenerate the nerve along the whole length of the conduit, semi flexibility and easy handling during surgery [3,4]. Furthermore, neurotrophic factors within the artificial nerve conduit help to align the nerve axons during regeneration as compared to the haphazard regeneration in autografts [5].…”

mentioning

confidence: 99%

Collagen-Coated Polylactic-Glycolic Acid (PLGA) Seeded with Neural-Differentiated Human Mesenchymal Stem Cells as a Potential Nerve Conduit

Sulong¹,

Hassan²,

Ng³

et al. 2014

Adv Clin Exp Med

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Background. Autologous nerve grafts to bridge nerve gaps pose various drawbacks. Nerve tissue engineering to promote nerve regeneration using artificial neural conduits has emerged as a promising alternative. Objectives. To develop an artificial nerve conduit using collagen-coated polylactic-glycolic acid (PLGA) and to analyse the survivability and propagating ability of the neuro-differentiated human mesenchymal stem cells in this conduit. Material and Methods. The PLGA conduit was constructed by dip-molding method and coated with collagen by immersing the conduit in collagen bath. The ultra structure of the conduits were examined before they were seeded with neural-differentiated human mesenchymal stem cells (nMSC) and implanted sub-muscularly on nude mice thighs. The non-collagen-coated PLGA conduit seeded with nMSC and non-seeded non-collagen-coated PLGA conduit were also implanted for comparison purposes. The survivability and propagation ability of nMSC was studied by histological and immunohistochemical analysis. Results. The collagen-coated conduits had a smooth inner wall and a highly porous outer wall. Conduits coated with collagen and seeded with nMSCs produced the most number of cells after 3 weeks. The best conduit based on the number of cells contained within it after 3 weeks was the collagen-coated PLGA conduit seeded with neuro-transdifferentiated cells. The collagen-coated PLGA conduit found to be suitable for attachment, survival and proliferation of the nMSC. Minimal cell infiltration was found in the implanted conduits where nearly all of the cells found in the cell seeded conduits are non-mouse origin and have neural cell markers, which exhibit the biocompatibility of the conduits. Conclusions. The collagen-coated PLGA conduit is biocompatible, non-cytotoxic and suitable for use as artificial nerve conduits (Adv Clin Exp Med 2014, 23, 3, 353-362).

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“…Así, en los sistemas experimentales de reparación como las cámaras de regeneración, las características de permeabilidad del material utilizado influyen en la tasa de sucesos y en la morfología del nervio periférico en regeneración (6,16,(20)(21)(22)(23). La cámara evaluada por nosotros se comporta como un sistema de compartimentos en donde la presencia o no de la ventana recubierta por la malla afecta la estructura morfológica de esta zona y el número de fibras a nivel distal.…”

Section: Discussionunclassified

“…estructura histológica del nervio regenerado en Esta es la vía que la ingeniería de tejidos está la 'Ona permeable, que es a lo postulandoen laactualidad,con la propuestade informado sobre sistemas completamente creación de prótesis bioartificiales, utilizando impermeables (24,25). implantes de células de Schwann v diversos t i~o s La entrada masiva de tejido cicatricial a la parte de'matrices incluidas en una cámára de material media de la cámara (zona permeable), da origen sintético biodegradable (23,(30)(31)(32). De esta a un tejido muy desorganizado (26).…”

Section: Discussionunclassified

“…Es importante señalar que nuestros hallazgos son contrarios a varios informes sobre otros modelos de tubos impermeables, en los cuales es mayor el número de fibras a nivel distal (18)(19)(20)(21)(22)(23)(24)(25). Los datos existentes en la literatura muestran que la entrada de células a las cámaras permeables incrementa el número de fibras CAMARA PERONEAL mielínicas y estimula la regeneración (6,28,29).…”

Section: Regeneracion De Nervio Perlferico En Camaras Permeablesunclassified

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Estudio de la regeneración de nervio periférico a través de cámaras permeables de silicona con poros de 10 y 60 micras

Alzate

Sutachán²,

Duque³

et al. 1999

biomedica

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ResumenSe entubó el nervio ciático de ratas, luego de un corte transverso, utilizando una cámara de silicona de 14 mm de largo que tenía dos ventanas de 8 mm de largo cubiertas con malla de 10 o 60 pm. Después de 6 meses, los animales se perfundieron con una mezcla de glutaraldehído y paraformaldehído, y se evaluó el aspecto microscópico de los nervios regenerados en dos niveles dentro de la cámara (3 mm y nivel medio) y 5 mm distal a ésta. Se contó el número de fibras mielínicas presentes en cada nivel y tratamiento, y se realizó una comparación entre ellos. Se encontró una importante desorganización del endoneurio a nivel medio de la cámara. Distalmente, los conteos de fibras mielínicas fueron muy bajos. No se observaron diferencias significativas entre los grupos con mallas de 10 y 60 pm. En conclusión, la presencia de poros de 10 o 60 pm no impide la entrada masiva de fibroblastos a la cámara, llevando a una marcada desorganización del nervio regenerado.Palabras clave: regeneración, nervio periférico, entubación, fibroblasto, permeabilidad.A study of peripheral nerve regeneration in permeable silicone chambers with 10 and 60 micrometer pores Abstract Rat sciatic nerves were transected and intubated with a fourteen millimeter lengthsilicone chamber, with two 8 mm windows covered with either a 10 or 60 pm mesh. After six months, the animals were perfused with a glutaraldehyde and paraformaldehyde mixture and the regenerated nerve histology was evaluated at two levels within the chamber (3 mm and midlevel) and 5 mm distal to the chamber. The myelinated nerve fibers were counted and compared for al1 levels and treatments. We found a disorganized endoneurium at midchamber level. Distally, a very low myelinated nerve fibers count was registered. No significant differences were observed between 10 and 60 vm meshes.We conclude that 10 or 60 pm meshes do not control the massive invasion of the chamber by fibroblasts, leading to a highly disorganized regenerated nerve.

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The development of bioartificial nerve grafts for peripheral-nerve regeneration

Cited by 194 publications

References 43 publications

A nanofibrous PHBV tube with Schwann cell as artificial nerve graft contributing to Rat sciatic nerve regeneration across a 30-mm defect bridge

A nanofibrous PHBV tube with Schwann cell as artificial nerve graft contributing to Rat sciatic nerve regeneration across a 30-mm defect bridge

Collagen-Coated Polylactic-Glycolic Acid (PLGA) Seeded with Neural-Differentiated Human Mesenchymal Stem Cells as a Potential Nerve Conduit

Estudio de la regeneración de nervio periférico a través de cámaras permeables de silicona con poros de 10 y 60 micras

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