The deformation mechanisms and fatigue behavior of prestrained Inconel 718 superalloy under fully-reversed fatigue were investigated at room temperature. Uniform gage section specimens were monotonically strained initially in either tension or compression up to 2% strain. Fullyreversed fatigue tests were subsequently conducted both on as-machined (no prestrain) and monotonically prestrained specimens in total strain control at a strain range of 0.8%. All tests were conducted in a computer-controlled servohydraulic test system at room temperature. Evolution of stress ranges and mean stresses observed during the fatigue tests on as-machined and prestrained specimens are reported. The specimens were sectioned and examined in a transmission electron microscope to reveal the deformation mechanisms under different types of loading.Results of the investigation and comparisons of the mechanisms of deformation observed under monotonic tensile strain, monotonic compressive strain, fully-reversed fatigue, tensile strain followed by fully-reversed fatigue, and compressive strain followed by fullyreversed fatigue are reported. Fatigue lives of the prestrained specimens are compared with life predictions by two different methods.