The development of induced pluripotent stem cell-derived mesenchymal stem/stromal cells from normal human and RDEB epidermal keratinocytes

Nakayama, Chihiro; Fujita, Yasuyuki; Matsumura, Wakana; Ujiie, Inkin; Takashima, Shota; Shinkuma, Satoru; Nomura, Toshifumi; Abe, Riichiro; Shimizu, Hiroshi

doi:10.1016/j.jdermsci.2018.06.004

Cited by 32 publications

(15 citation statements)

References 43 publications

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“…It can be constructed from natural and/or synthetic biomaterials, either materials that remain stable in a biological environment or materials that degrade in the human body [ 2 ]. Several techniques have been adopted for their constructions, but the four main approaches that are widely used include: (i) Sheets of cells secreting ECM [ 21 ]; pre-made porous scaffolds of synthetic, natural, and biodegradable biomaterial; (ii) decellularized ECM scaffolds, and (iii) cells entrapped in hydrogels [ 9 ]. The main objective of the scaffold is to represent the matrix as similar as possible to the native ECM as all cells are in close contact with ECM, which provides structural support to cells and tissues, stimulating migration proliferation, apoptosis, survival, and differentiation [ 2 ].…”

Section: Techniques Of Skin Tissue Engineeringmentioning

confidence: 99%

Synergistic Effect of Biomaterial and Stem Cell for Skin Tissue Engineering in Cutaneous Wound Healing: A Concise Review

2021

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Skin tissue engineering has made remarkable progress in wound healing treatment with the advent of newer fabrication strategies using natural/synthetic polymers and stem cells. Stem cell therapy is used to treat a wide range of injuries and degenerative diseases of the skin. Nevertheless, many related studies demonstrated modest improvement in organ functions due to the low survival rate of transplanted cells at the targeted injured area. Thus, incorporating stem cells into biomaterial offer niches to transplanted stem cells, enhancing their delivery and therapeutic effects. Currently, through the skin tissue engineering approach, many attempts have employed biomaterials as a platform to improve the engraftment of implanted cells and facilitate the function of exogenous cells by mimicking the tissue microenvironment. This review aims to identify the limitations of stem cell therapy in wound healing treatment and potentially highlight how the use of various biomaterials can enhance the therapeutic efficiency of stem cells in tissue regeneration post-implantation. Moreover, the review discusses the combined effects of stem cells and biomaterials in in vitro and in vivo settings followed by identifying the key factors contributing to the treatment outcomes. Apart from stem cells and biomaterials, the role of growth factors and other cellular substitutes used in effective wound healing treatment has been mentioned. In conclusion, the synergistic effect of biomaterials and stem cells provided significant effectiveness in therapeutic outcomes mainly in wound healing improvement.

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Section: Techniques Of Skin Tissue Engineeringmentioning

confidence: 99%

Synergistic Effect of Biomaterial and Stem Cell for Skin Tissue Engineering in Cutaneous Wound Healing: A Concise Review

2021

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“…Nakayama et al [57] successfully established hiPSC-MSC from keratinocytes of healthy individuals and patients with epidermolysis bullosa and studied their wound healing potential in a nude mouse model [57]. With subcutaneous and intravenous delivery, hiPSC-MSC successfully secreted human type VII collagen at the dermal-epidermal junction.…”

Section: Human-induced Pluripotent-derived Mesenchymal Stem Cellsmentioning

confidence: 99%

The potential and limitations of induced pluripotent stem cells to achieve wound healing

et al. 2019

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Wound healing is the physiologic response to a disruption in normal skin architecture and requires both spatial and temporal coordination of multiple cell types and cytokines. This complex process is prone to dysregulation secondary to local and systemic factors such as ischemia and diabetes that frequently lead to chronic wounds. Chronic wounds such as diabetic foot ulcers are epidemic with great cost to the healthcare system as they heal poorly and recur frequently, creating an urgent need for new and advanced therapies. Stem cell therapy is emerging as a potential treatment for chronic wounds, and adult-derived stem cells are currently employed in several commercially available products; however, stem cell therapy is limited by the need for invasive harvesting techniques, immunogenicity, and limited cell survival in vivo. Induced pluripotent stem cells (iPSC) are an exciting cell type with enhanced therapeutic and translational potential. iPSC are derived from adult cells by in vitro induction of pluripotency, obviating the ethical dilemmas surrounding the use of embryonic stem cells; they are harvested non-invasively and can be transplanted autologously, reducing immune rejection; and iPSC are the only cell type capable of being differentiated into all of the cell types in healthy skin. This review focuses on the use of iPSC in animal models of wound healing including limb ischemia, as well as their limitations and methods aimed at improving iPSC safety profile in an effort to hasten translation to human studies.

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“…So far, there are myriad studies confirmed improved wound healing and angiogenesis via sole‐ or co‐delivery of human iPSCs (hiPSCs)‐derived endothelial cells (ECs), SMCs, MSCs with or without fibroblasts 66,74‐80 . As an example, it was reported in the study of Shen et al that hiPSC‐derived vascular cells increased vascular formation during wound healing in Diabetic foot ulcer patient due to their high capability of differentiation to the two major vascular cell populations of endothelial cells and pericytes 81 .…”

Section: Treatment Of Chronic Wounds Using Angiogenic Approachesmentioning

confidence: 99%

Skin wound healing assisted by angiogenic targeted tissue engineering: A comprehensive review of bioengineered approaches

Nour

Imani

Chaudhry

et al. 2020

J Biomedical Materials Res

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Skin injuries and in particular, chronic wounds, are one of the major prevalent medical problems, worldwide. Due to the pivotal role of angiogenesis in tissue regeneration, impaired angiogenesis can cause several complications during the wound healing process and skin regeneration. Therefore, induction or promotion of angiogenesis can be considered as a promising approach to accelerate wound healing. This article presents a comprehensive overview of current and emerging angiogenesis induction methods applied in several studies for skin regeneration, which are classified into the cell, growth factor, scaffold, and biological/chemical compound‐based strategies. In addition, the advantages and disadvantages of these angiogenic strategies along with related research examples are discussed in order to demonstrate their potential in the treatment of wounds.

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The development of induced pluripotent stem cell-derived mesenchymal stem/stromal cells from normal human and RDEB epidermal keratinocytes

Abstract: We successfully established iPSC-derived MSCs from keratinocytes (KC-iPSC-MSCs) of a normal human and an RDEB patient. KC-iPSC-MSCs may have potential in therapies for RDEB.

Cited by 32 publications

References 43 publications

Synergistic Effect of Biomaterial and Stem Cell for Skin Tissue Engineering in Cutaneous Wound Healing: A Concise Review

Synergistic Effect of Biomaterial and Stem Cell for Skin Tissue Engineering in Cutaneous Wound Healing: A Concise Review

The potential and limitations of induced pluripotent stem cells to achieve wound healing

Skin wound healing assisted by angiogenic targeted tissue engineering: A comprehensive review of bioengineered approaches

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