The developmental transcriptome atlas of the spoon worm <i>Urechis unicinctus</i> (Echiurida: Annelida)

Park, Chungoo; Han, Yong; Lee, Sung-Gwon; Ry, Kyoung Bin; Oh, Jooseong; Kern, Elizabeth M.A.; Park, Joong Ki; Cho, Sung Jin

doi:10.1093/gigascience/giy007

Cited by 17 publications

(13 citation statements)

References 17 publications

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“…In comparison to other annelid transcriptomes, which found around 80% of complete BUSCOs in Pygospio elegans (Heikkinen et al. 2017) and Urechis unicinctus (Park et al. 2018), and approximately 60% in Sabellaria alveolata and Phragmatopoma caudata (Buffet et al.…”

Section: Resultsmentioning

confidence: 86%

Delegating Sex: Differential Gene Expression in Stolonizing Syllids Uncovers the Hormonal Control of Reproduction

Álvarez‐Campos

Kenny

Verdes

et al. 2018

Genome Biology and Evolution

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Stolonization in syllid annelids is a unique mode of reproduction among animals. During the breeding season, a structure resembling the adult but containing only gametes, called stolon, is formed generally at the posterior end of the animal. When stolons mature, they detach from the adult and gametes are released into the water column. The process is synchronized within each species, and it has been reported to be under environmental and endogenous control, probably via endocrine regulation. To further understand reproduction in syllids and to elucidate the molecular toolkit underlying stolonization, we generated Illumina RNA-seq data from different tissues of reproductive and nonreproductive individuals of Syllis magdalena and characterized gene expression during the stolonization process. Several genes involved in gametogenesis (ovochymase, vitellogenin, testis-specific serine/threonine-kinase), immune response (complement receptor 2), neuronal development (tyrosine-protein kinase Src42A), cell proliferation (alpha-1D adrenergic receptor), and steroid metabolism (hydroxysteroid dehydrogenase 2) were found differentially expressed in the different tissues and conditions analyzed. In addition, our findings suggest that several neurohormones, such as methyl farnesoate, dopamine, and serotonin, might trigger stolon formation, the correct maturation of gametes and the detachment of stolons when gametogenesis ends. The process seems to be under circadian control, as indicated by the expression patterns of r-opsins. Overall, our results shed light into the genes that orchestrate the onset of gamete formation and improve our understanding of how some hormones, previously reported to be involved in reproduction and metamorphosis processes in other invertebrates, seem to also regulate reproduction via stolonization.

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Section: Resultsmentioning

confidence: 86%

Delegating Sex: Differential Gene Expression in Stolonizing Syllids Uncovers the Hormonal Control of Reproduction

Álvarez‐Campos

Kenny

Verdes

et al. 2018

Genome Biology and Evolution

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“…Data from U. unicinctus larval transcriptomes [ 29 , 60 ] and adult transcriptome [ 61 ] were used in this study. To search for transcripts encoding putative neuropeptides or peptide hormone precursor proteins in U. unicinctus , the homologous sequences previously identified in annelids ( Platynereis dumerilii [ 30 ], Capitella capitate [ 44 ] and Helobdella robusta [ 44 ]), molluscs ( Patinopecten yessoensis [ 45 ], Pinctata fucata [ 62 ], Lottia gigantea [ 63 ], Crassostrea gigas [ 62 ] and Deroceras reticulatum [ 64 ]) and echinoderms ( Asterias rubens [ 65 ], Ophionotus Victoria [ 66 ], Strongylocentrotus purpuratus [ 67 ] and Apostichopus japonicus [ 68 ]) were downloaded from NCBI and used as queries in tBLASTn searches of the assembled U. unicinctus transcriptome database using BioEdit v7.0.9 with an E-value cutoff of 1e-5.…”

Section: Methodsmentioning

confidence: 99%

Identification of the neuropeptide precursor genes potentially involved in the larval settlement in the Echiuran worm Urechis unicinctus

et al. 2020

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Background In marine invertebrate life cycles, which often consist of planktonic larval and benthonic adult stages, settlement of the free-swimming larva to the sea floor in response to environmental cues is a key life cycle transition. Settlement is regulated by a specialized sensory–neurosecretory system, the larval apical organ. The neuroendocrine mechanisms through which the apical organ transduces environmental cues into behavioral responses during settlement are not fully understood yet. Results In this study, a total of 54 neuropeptide precursors (pNPs) were identified in the Urechis unicinctus larva and adult transcriptome databases using local BLAST and NpSearch prediction, of which 10 pNPs belonging to the ancient eumetazoa, 24 pNPs belonging to the ancient bilaterian, 3 pNPs belonging to the ancient protostome, 9 pNPs exclusive in lophotrochozoa, 3 pNPs exclusive in annelid, and 5 pNPs only found in U. unicinctus. Furthermore, four pNPs (MIP, FRWamide, FxFamide and FILamide) which may be associated with the settlement and metamorphosis of U. unicinctus larvae were analysed by qRT-PCR. Whole-mount in situ hybridization results showed that all the four pNPs were expressed in the region of the apical organ of the larva, and the positive signals were also detected in the ciliary band and abdomen chaetae. We speculated that these pNPs may regulate the movement of larval cilia and chaeta by sensing external attachment signals. Conclusions This study represents the first comprehensive identification of neuropeptides in Echiura, and would contribute to a complete understanding on the roles of various neuropeptides in larval settlement of most marine benthonic invertebrates.

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“…Data from U. unicinctus larval transcriptomes [59,60] and adult transcriptome [61] were used in this study.…”

Section: Identification Functional Annotation and Sequence Alignmentmentioning

confidence: 99%

Identification of the Neuropeptide Precursor Genes Potentially Involved in the Larval Settlement in the Echiuran Worm Urechis unicinctus

Hou

Qin

Wei

et al. 2020

Preprint

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Background: In marine invertebrate life cycles, which often consist of planktonic larval and benthonic adult stages, settlement of the free-swimming larva to the sea ﬂoor in response to environmental cues is a key life cycle transition. Settlement is regulated by a specialized sensory–neurosecretory system, the larval apical organ. The neuroendocrine mechanisms through which the apical organ transduces environmental cues into behavioral responses during settlement are not fully understood yet.Results: In this study, a total of 54 neuropeptide precursors (pNPs) were identified in the Urechis unicinctus larva and adult transcriptome databases using local BLAST and de novo prediction, of which 10 pNPs belonging to the ancient eumetazoa, 23 pNPs belonging to the ancient bilaterian, 3 pNPs belonging to the ancient protostome, 10 pNPs exclusive in lophotrochozoa, 3 pNPs exclusive in annelid, and 5 pNPs only found in U. unicinctus. Furthermore, four pNPs (MIP, FRWamide, FxFamide and FILamide) which may be associated with the settlement and metamorphosis of U. unicinctus larvae were verified successfully by qRT-PCR. Whole-mount in situ hybridization results showed that all the four pNPs were expressed in the region of the apical organ of the larva, and the positive signals were also detected in the ciliary band and abdomen chaetae. We speculated that these pNPs may regulate the movement of larval cilia and chaeta by sensing external attachment signals.Conclusions: This study represents the ﬁrst comprehensive identiﬁcation of neuropeptides in Echiura, and would contribute to a complete understanding on the roles of various neuropeptides in larval settlement of most marine benthonic invertebrates.

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The developmental transcriptome atlas of the spoon worm Urechis unicinctus (Echiurida: Annelida)

Cited by 17 publications

References 17 publications

Delegating Sex: Differential Gene Expression in Stolonizing Syllids Uncovers the Hormonal Control of Reproduction

Delegating Sex: Differential Gene Expression in Stolonizing Syllids Uncovers the Hormonal Control of Reproduction

Identification of the neuropeptide precursor genes potentially involved in the larval settlement in the Echiuran worm Urechis unicinctus

Identification of the Neuropeptide Precursor Genes Potentially Involved in the Larval Settlement in the Echiuran Worm Urechis unicinctus

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