The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells

Abstract

Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

Utilization of Electrolyte Solution in Nanotube Formation on Ti-6Al-4V Metal Alloy

“…Nanotube on pure TiO2 would only be formed on pure Ti has been reported as the main component of the nanotube that was formed on Ti6Al-4V metal alloy, nanotube that was formed also contained vanadium, aluminum, and fluorine from the electrolyte solution such as HF/HNO3/H2O, which was used before anodization. During the process of nanotube TiO2 nanotube formation not only Ti that was oxidized but also Al and V, forming Al2O3 and V2O5 [16]. The reaction as followed: in ethylene glycol:distilled water (9:1) which was the best condition for oxide layered nanotube , as comparable with the research that has been done by Pawelska and Cydzik [17].…”

Current Advances in TiO2-Based Nanostructure Electrodes for High Performance Lithium Ion Batteries

“…Although several binary and ternary mixed oxide nanotubes were fabricated by the anodization of Ti-Fe [148], Ti-Ta, Ti-Nb [175], Ti-Pd [176], Ti-Al [177], Ti-Mo [178], Ti-V [179], Ti-W [180], Ti-Mn [181], Ti-Ni [182], Ti-Mo-Ni [183], and Ti-6Al-4V [184] alloys, only some of them were applied as electrodes in lithium ion batteries. As an example, well-ordered mixed Ti-Mn-O nanotubes were grown on the surface of a Ti-8Mn alloy by anodization [181].…”

Combined Infection Control and Enhanced Osteogenic Differentiation Capacity on Additive Manufactured Ti‐6Al‐4V are Mediated via Titania Nanotube Delivery of Novel Biofilm Inhibitors

“…Our previous work based on anodization of Ti‐6Al‐4V and other publications demonstrated a reduction in Ti, Al, and V content compared with polished control, whereas O and F were detected on anodized Ti‐6Al‐4V surfaces. [ 27,71,72 ] However, F content was not expected to influence biocompatibility for application of anodized surfaces since in vitro and in vivo studies have validated the supportive effect on bone regeneration of anodized surfaces. [ 72,73 ] Furthermore, fluoride‐modified dental implants via electrochemical etching illustrated the significant improvement of bone regeneration and has been widely used in clinical applications, even though it is difficult to distinguish the individual contribution made by surface texture or chemical composition.…”