The different structures containing tight junction proteins in epidermal and other stratified epithelial cells, including squamous cell metaplasia

Schlüter, Holger; Moll, Ingrid; Wolburg, Hartwig; Franke, Werner W.

doi:10.1016/j.ejcb.2007.01.001

Cited by 66 publications

(62 citation statements)

References 58 publications

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“…9 The TJ strand has been said to be restricted to wherever occludin exists, even if the function of occludin is still unclear. [23][24][25][26][27] Even in the stomach and intestine, the expression pattern of the claudins is broad, running from the apical to basolateral membrane, while that of occludin is only dense at TJs in normal tissues. 12,28 Figure 5 Influence of acid on TJ protein localization in ALI cells.…”

Section: Squamous Epithelial Barrier Function T Oshima Et Almentioning

confidence: 99%

Acid modulates the squamous epithelial barrier function by modulating the localization of claudins in the superficial layers

Oshima

Koseki

Chen

et al. 2012

Laboratory Investigation

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Acid is a major cause of gastro-esophageal reflux disease. However, the influence of acid on the esophageal stratified epithelial barrier function and tight junction (TJ) proteins is not fully understood. Here, we explore the influence of acid on barrier function and TJ proteins using a newly developed model of the esophageal-like squamous epithelial cell layers that employs an air-liquid interface (ALI) system. Barrier function was determined by measuring trans-epithelial electrical resistance (TEER) and diffusion of paracellular tracers. TJ-related protein (claudin-1, claudin-4, occludin and ZO-1) expression and localization was examined by immunofluorescent staining, and by western blotting of 1% NP-40 soluble and insoluble fractions. We also examined the influence of acid (pH 2-4) on the barrier created by these cells. The in vitro ALI culture system showed a tight barrier (1500-2500 O . cm 2 ) with the expression of claudin-1, claudin-4, occludin and ZO-1 in the superficial layers. Claudin-1, claudin-4, occludin and ZO-1 were detected as dots and whisker-like lines in the superficial layers, and as a broad line in the suprabasal layers. These localization patterns are similar to those in the human esophagus. On day 7 under ALI culture, TJ proteins were detected in the superficial layers with functional properties, including decreased permeability and increased TEER. Dilated intercellular spaces were detected at the suprabasal cell layers even under the control conditions of ALI cells. pH 2 acid on the apical side significantly reduced the TEER in ALI-cultured cells. This decrease in TEER by the acid was in parallel with the decreased amount of detergent-insoluble claudin-4. Claudin-4 delocalization was confirmed by immunofluorescent staining. In conclusion, TJs are located in the superficial layers of the esophagus, and acid stimulation disrupts barrier function, at least in part by modulating the amount and localization of claudin-4 in the superficial layers.

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Section: Squamous Epithelial Barrier Function T Oshima Et Almentioning

confidence: 99%

Acid modulates the squamous epithelial barrier function by modulating the localization of claudins in the superficial layers

Oshima

Koseki

Chen

et al. 2012

Laboratory Investigation

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“…Accordingly, TJ-related structures have been identified by EM below and above the SG, especially in the interdesmosomal region of various stratified epithelia. 9,17 Over the past 2 decades outstanding progress has been made in this field, but much remains to be done to fully appreciate the role epidermal TJs play in human health and disease. In this paper we will review what is known about the epidermal TJ barrier function and composition, its regulation by the immune system as well as the relevance of these observations for dermatologic diseases.…”

Section: E974451-2mentioning

confidence: 99%

“…Growing body of evidence suggests that the TJ proteins are involved in important steps of tumorigenesis, such as apoptosis, alteration of cell-cell adhesion and tumor invasion (for a review see 17 ). Malignant melanoma (MM) is the most lethal skin cancer.…”

Section: Tj Abnormalities In Human Skin Disordersmentioning

confidence: 99%

Epidermal tight junctions in health and disease

et al. 2014

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“…The occurrence of TRIC-b is hypothesized in basal cell layers of keratinocyte cultures (Schlü ter et al, 2007). TRIC-c (442 aa) is predicted to be a two-transmembrane domain protein with an alternatively spliced exon two.…”

Section: Introductionmentioning

confidence: 99%

Tricellulin Forms a Barrier to Macromolecules in Tricellular Tight Junctions without Affecting Ion Permeability

Krug

Amasheh

Richter

et al. 2009

MBoC

308

322

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Tricellulin is a tight junction protein localized in tricellular tight junctions (tTJs), the meeting points of three cells, but also in bicellular tight junctions (bTJs). To investigate its specific barrier functions in bTJs and tTJs, TRIC-a was expressed in low-level tricellulin-expressing cells, and MDCK II, either in all TJs or only in tTJs. When expressed in all TJs, tricellulin increased paracellular electrical resistance and decreased permeability to ions and larger solutes, which are associated with enhanced ultrastructural integrity of bTJs toward enhanced strand linearity. In tTJs in contrast, ultrastructure was unchanged and tricellulin minimized permeability to macromolecules but not to ions. This paradox is explained by properties of the tTJ central tube which is wide enough for passage of macromolecules, but too rare to contribute significantly to ion permeability. In conclusion, at low tricellulin expression the tTJ central tube forms a pathway for macromolecules. At higher expression, tricellulin forms a barrier in tTJs effective only for macromolecules and in bTJs for solutes of all sizes.

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The different structures containing tight junction proteins in epidermal and other stratified epithelial cells, including squamous cell metaplasia

Cited by 66 publications

References 58 publications

Acid modulates the squamous epithelial barrier function by modulating the localization of claudins in the superficial layers

Acid modulates the squamous epithelial barrier function by modulating the localization of claudins in the superficial layers

Epidermal tight junctions in health and disease

Tricellulin Forms a Barrier to Macromolecules in Tricellular Tight Junctions without Affecting Ion Permeability

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