The Differential Effect of Cannabidiol on the Composition and Physicochemical Properties of Keratinocyte and Fibroblast Membranes from Psoriatic Patients and Healthy People

Abstract

The development of psoriasis is accompanied by oxidative stress, which can modify the components of skin cells. Therefore, the aim of this study was to evaluate the effect of cannabidiol (CBD), an antioxidant and anti-inflammatory phytocannabinoid, on the composition and physicochemical properties of the membranes of healthy and psoriatic keratinocytes and fibroblasts exposed to ultraviolet A (UVA) and ultraviolet B (UVB) radiation. In psoriasis-altered cells, decreased levels of the main groups of phospholipids and increased levels of sialic acid and malondialdehyde (MDA), a lipid peroxidation product, as well as negative charge of cell membranes compared to non-diseased cells, were found. On the other hand, UVA/B radiation increased the levels of phospholipids and MDA in both groups of cells. Moreover, psoriatic cells were characterized by lower levels of sialic acid and negative charge of cell membranes, while non-diseased cells showed the opposite response. The CBD treatment intensified some of the changes (phospholipid content and membrane charge) caused by the radiation of psoriatic cells, while it prevented these changes in the cells of healthy people. The results of this study indicate that CBD can prevent structural and functional changes to the membranes of healthy skin cells during phototherapy for psoriasis.

Phytocannabinoids Stimulate Rejuvenation and Prevent Cellular Senescence in Human Dermal Fibroblasts

“…Similarly, previous studies have demonstrated beneficial anti-aging effects on fibroblasts with the use of cannabis extracts which included phytocannabinoids; however, pure THC and CBD were not tested, and the compounds that induce these anti-aging effects were not identified [ 18 , 19 , 20 , 21 ]. In addition, some studies have shown CBD exposure on dermal fibroblasts prevented structural changes caused by psoriasis [ 22 ] and ameliorated proteome changes after UV damage [ 23 ]. There, however, remains a lack of knowledge on the anti-aging and rejuvenation effects of THC and CBD on healthy and aged human dermal fibroblasts.…”

Melatonin and Indole-3-Propionic Acid Reduce Oxidative Damage to Membrane Lipids Induced by High Iron Concentrations in Porcine Skin

“…We recently documented that increased levels of lipid peroxidation products in the epidermis results from smoking [ 6 ] or obesity [ 7 ]. Additionally, direct skin exposure to pro-oxidants, such as ultraviolet UVA and UVB radiation, enhanced oxidative damage of membrane lipids in both normal and psoriatic keratinocytes and fibroblasts [ 8 ].…”

The cannabinoid receptors system in horses: Tissue distribution and cellular identification in skin

“… 13 , 16 , 17 Accordingly, peripheral application of cannabinoid‐related compounds has shown therapeutic benefits, decreasing associated comorbidities and reconstituting proper skin tissue architecture and physiology. 18 , 19 …”

“…13,16,17 Accordingly, peripheral application of cannabinoid-related compounds has shown therapeutic benefits, decreasing associated comorbidities and reconstituting proper skin tissue architecture and physiology. 18,19 These CBR-based treatments recently have attracted considerable attention in equine veterinary medicine after their use in other vertebrate species, including dogs, cats, and pigs. [20][21][22][23][24] Unfortunately, knowledge about the ECS in horses is still limited to dorsal root ganglion (DRG) neurons and equine genome sequencing analysis.…”

“…In turn, experimental studies have shown that disordered cutaneous homeostasis might be established in a CBRs‐dependent manner 13,16,17 . Accordingly, peripheral application of cannabinoid‐related compounds has shown therapeutic benefits, decreasing associated comorbidities and reconstituting proper skin tissue architecture and physiology 18,19 …”