The Differentiation of Various Strains of Clostridium botulinum and Transformation Experiments with Type E

Skulberg, A.; Hausken, O. W.

doi:10.1111/j.1365-2672.1965.tb04538.x

Cited by 3 publications

(2 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Germination was linear (r = 0.99), with a rate of 0.77% min-' for the first 60 min of incubation. A total of 88% of the spores germinated within 6 h of incubation, and 97% germinated after 24 h of incubation at 37°C. When spores were germinated in media containing 2% salt, only a slight influence was observed.…”

Section: Resultsmentioning

confidence: 99%

“…Both the genesis and instability of strain Ba410 can be explained by selection of either preexistent "commensal contaminants" (12) or genetic variants from the original Baphis culture. These two hypotheses have been discussed with regard to apparent transformation in opaque sporulating (hypotoxigenic) and toxigenic cultures of C. botulinum types A, B, C (24), and E (12,24). Both mechanisms explain the isolation of salt-tolerant strains equally well.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of a halo-acid-tolerant variant of Clostridium botulinum B-aphis

Montville¹

1984

Appl Environ Microbiol

View full text Add to dashboard Cite

Clostridium botulinum B-aphis spores plated on medium containing 4% salt at pH 6.0 yielded colonies at a frequency of ca. 1 in 106. A subculture of one of these colonies, designated strain Ba410, was compared with the parent strain, B-aphis, for a variety of traits. After 7 days of incubation at 37°C, strain Ba410 grew in medium containing 7% NaCl, whereas strain B-aphis could not grow in salt concentrations greater than 5%. The strains also differed in cellular and colonial morphology. After exponential growth in the basal medium was completed, lysis of both strains was pH dependent; in media containing salt, lysis of Ba410 cells was pH independent. Strain Ba410 was more proteolytic than strain B-aphis in conditions of low pH and high salt, so that its toxin could be detected by the mouse assay. In a medium containing alanine and cysteine, the germination rate of Baphis was 0.77% min-', whereas that of Ba410 was 0.14% min-; 2% salt inhibited the germination of Ba410 but not B-aphis.

show abstract

Section: Resultsmentioning

confidence: 99%