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Spores of Clostridium botuliwum type E have been screened for resistance to heating in aqueous suspension a t 60, 70 and SO". D178 ranged from <0.33 min to 1.25 min. z values of eight strains were between 9.0 and 10.7, and for one strain was 7.4.Radiation survivor curves have been plotted for nine strains, and a further nine strains screened. The "shoulder" extends to about 0.4 megarad, whereafter the curve becomes exponential within a D value from 0.065 to 0.16 megarad.* Irradiations were made on spores in RCM at 0-3". and 108 (a Russian strain obtained from Prof. K. F. Meyer) were subjected to 70, 75, and 80" by Ohye & Scott (1957) and their decimal reduction ( D ) times corresponded well with those of Dolman ( 1 9 5 7~) . Schmidt (1964) heated strain Minneapolis a t temperatures between 74.5" and 85.5") obtaining D times midway between those for the strains examined by Ohye & Scott (1957). The heat resistance of strain Tenno

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Cl. botulinum type E the samples also contained large numbers of Cb. znel4hii. References BONDE, G. J. (1962). Bacterial indicators of water pollution. A study of quantitative estimation. Thesis, University of Copenhagen. Journal of Applied Bacteriology, VoI. 28, Part 1 00 M 4 W Y u m

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