The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates

Lam, Kwok Wai; Si, Kai-wei; Wu, Xiyang; Tang, Shuze; Sun, Xiaohui; Kwan, Hoi Shan; Cheung, Peter C.K.

doi:10.1016/j.jbiotec.2018.07.009

Cited by 9 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A range of possible enzymes were identified to be involved in this β-glucan biosynthesis. 22 Proteins. As an important component of plants and animals, proteins and peptides possess important biological activities.…”

Section: ■ Chemical Compositionmentioning

confidence: 99%

See 1 more Smart Citation

Research on a Specialty Mushroom (Pleurotus tuber-regium) as a Functional Food: Chemical Composition and Biological Activities

Lin

Wang

Lam

et al. 2020

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Pleurotus tuber-regium (PTR) is an edible specialty mushroom that has attracted growing interest recently because of its sensory attributes, high nutritional values, and important medicinal properties. PTR is rich in bioactive polysaccharides, proteins with essential amino acids, essential fatty acids, dietary fiber, minerals, and vitamins. Current studies have shown that the nutrients and bioactive ingredients of PTR contribute to their antitumor, antihypercholesterolemic, antihypertensive, antiobesity, hepatic-protective, antimicrobial, antioxidant, and prebiotic activities, indicating that PTR is a promising functional food and nutraceutical. In this review, the chemical constituents and physiological functions of PTR are summarized, which provide the scientific basis to support the further research and development of its application in the food and pharmaceutical industries.

show abstract

Section: ■ Chemical Compositionmentioning

confidence: 99%

“…Recently, the diploid genome of PTR wild strain was sequenced and successfully annotated, which provides important references for understanding how PTR transforms the substrate biomass into β-glucans during sclerotia formation. A range of possible enzymes were identified to be involved in this β-glucan biosynthesis …”

Section: Chemical Compositionmentioning

confidence: 99%

Research on a Specialty Mushroom (Pleurotus tuber-regium) as a Functional Food: Chemical Composition and Biological Activities

Lin

Wang

Lam

et al. 2020

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Approximately, 527 × coverage data were acquired. The monokaryon genome is bigger in size than the dikaryon genome of this previously sequenced mushroom using the Illumina platform ( Lam et al, 2018 ). The genome sequenced in our study is similar in size to the genomes of several other Agaricales species, including L. rhinocerotis (34.31 Mb) ( Yap et al, 2014 ) and P. ostreatus (34.9 Mb) ( Qu et al, 2016 ) but smaller than that of W. cocos (50.48 Mb) ( Floudas et al, 2012 ), P. tuoliensis (48.2 Mb), and P. eryngii (49.9 Mb) ( Zhang Z. et al, 2018 ).…”

Section: Resultsmentioning

confidence: 95%

Genome and Comparative Transcriptome Dissection Provide Insights Into Molecular Mechanisms of Sclerotium Formation in Culinary-Medicinal Mushroom Pleurotus tuber-regium

Sun

Zhang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Pleurotus tuber-regium is an edible and medicinal sclerotium-producing mushroom. The sclerotia of this mushroom also serve as food and folk medicine. Based on the description of its monokaryon genome, sequenced with Illumina and PacBio sequencing technologies, comparative transcriptomic analysis using RNA sequencing (RNA-seq) was employed to study its mechanism of sclerotium formation. The de novo assembled genome is 35.82 Mb in size with a N50 scaffold size of 4.29 Mb and encodes 12,173 putative proteins. Expression analysis demonstrated that 1,146 and 1,249 genes were upregulated and downregulated with the formation of sclerotia, respectively. The differentially expressed genes were associated with substrate decomposition, the oxidation-reduction process, cell wall synthesis, and other biological processes in P. tuber-regium. These genomic and transcriptomic resources provide useful information for the mechanism underlying sclerotium formation in P. tuber-regium.

show abstract

“…Previous studies have focused on the research of P. tuber-regium reproduction ( Fasidi & Ekuere, 1993 ; Okhuoya & Etugo, 1993 ; Bamigboye, Oloke, & Dames, 2019 ), nutrition ( Oyetayo & Akindahunsi, 2005 ; Ohiri, 2018 ), pharmacological value ( Wu, Lu, Jiang, Li, & Huang, 2014 ; Okolo, Orisakwe, & Siminialayi, 2018 ), and molecular biology ( Huang, Lin, Cheung, & Wu, 2011 ; Liu et al, 2018b ). With the completion of the diploid genome sequencing ( Lam et al, 2018 ) and the construction of Agrobacterium tumefaciens -mediated transformation system ( Liu et al, 2018b ) in P. tuber-regium , there is an increasing attention on the molecular biological research of important functional genes as it provides necessary information about fungal development and metabolite synthesis.…”

Section: Introductionmentioning

confidence: 99%

Selection and validation of endogenous reference genes for RT-qPCR normalization in different stresses and tissues of the tiger milk mushroom, <i>Pleurotus tuber-regium</i>

Sun

et al. 2021

Mycoscience

View full text Add to dashboard Cite

Pleurotus tuber-regium is a sclerotium-producing mushroom with pharmacological and nutritional value. Many researches applied molecular biological strategies that allow analyzing numerous genes expressed in response to different growth stresses or tissues. In order to capture accurate and reliable results in gene expression studies, it is necessary to select appropriate internal reference genes. They must have relatively uniform expression in the majority of tested samples, thereby reducing the impact of the sample itself on the RT-qPCR results. The selection and validation of 14 candidate reference genes, including α-tub, β-tub, γ-tub, GAPDH, Tif-5a, Tef-1α, ATPase, acyl-CoA, U2ribo, S/G, Wlp, VtpAsE, E3upl, and Sdh, were carried out for gene expression analysis in P. tuber-regium mycelia in response to different temperatures, drought levels, and salinity shifts, fruitbody, and sclerotium. Four statistical algorithms, NormFinder, geNorm, BestKeeper, and RefFinder, were recruited to evaluate the transcription stability of candidate reference genes. The RT-qPCR expression stability analysis indicated that the E3upl and Tif-5a were the most stable expressed genes among all experimental samples, so the combination of these two reference genes is suitable for the analysis of gene expression patterns in P. tuber-regium.

show abstract

The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates

Cited by 9 publications

References 15 publications

Research on a Specialty Mushroom (Pleurotus tuber-regium) as a Functional Food: Chemical Composition and Biological Activities

Research on a Specialty Mushroom (Pleurotus tuber-regium) as a Functional Food: Chemical Composition and Biological Activities

Genome and Comparative Transcriptome Dissection Provide Insights Into Molecular Mechanisms of Sclerotium Formation in Culinary-Medicinal Mushroom Pleurotus tuber-regium

Selection and validation of endogenous reference genes for RT-qPCR normalization in different stresses and tissues of the tiger milk mushroom, <i>Pleurotus tuber-regium</i>

Contact Info

Product

Resources

About