The discovery of a potent pure chemical wound-healing accelerator

Prudden, John F.; Migel, Peter; Hanson, Paul R.; Friedrich, Louis; Balassa, Leslie

doi:10.1016/0002-9610(70)90175-3

Cited by 170 publications

(75 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The absolute configuration of glucosamine was determined by Haworth in 1939 (Irvine & Hynd, 1912). Only in 1970, Prudded`s research concluded that glucosamine and N-acetyl-D-glucosamine accelerated wound healing processes (Prudden et al, 1970).…”

Section: Chitosan Historymentioning

confidence: 99%

Chitosan as a starting material for wound healing applications

Patrulea

Ostafe

Borchard

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

487

215

View full text Add to dashboard Cite

Abstract:Chitosan and its derivatives have attracted great attention due to their properties beneficial for application to wound healing. The main focus of the present review is to summarize studies involving chitosan and its derivatives, especially N,N,N-trimethylchitosan (TMC), N,O-carboxymethyl-chitosan (CMC) and O-carboxymethyl-N,N,Ntrimethyl-chitosan (CMTMC), used to accelerate wound healing. Moreover, formulation strategies for chitosan and its derivatives, as well as their in vitro, in vivo and clinical applications in wound healing are described.

show abstract

Section: Chitosan Historymentioning

confidence: 99%

Chitosan as a starting material for wound healing applications

Patrulea

Ostafe

Borchard

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

487

215

View full text Add to dashboard Cite

show abstract

“…66(12): 1595-1598, 2004 Chitin and chitosan have been widely studied in both engineering and medicine. In the field of medicine, the effects of chitin and chitosan on wound healing have been the focus of great attention since Prudden et al published a paper in 1970 stating that these agents accelerate wound healing [15]. Numerous reports describe the stimulatory effects of chitin and chitosan on tissue reactions involved in wound healing [1,[3][4][5][7][8][9][10][11][12][13].…”

mentioning

confidence: 99%

Effects of Chitin and Chitosan on Collagen Synthesis in Wound Healing

et al. 2004

View full text Add to dashboard Cite

ABSTRACT. Collagen synthesis was evaluated by measuring prolyl hydroxylase (PHL) activity induced within rat granulation tissue by a polyester non-woven fabric (NWF, 1 × 1 cm, 0.6 mm in thickness) impregnated with a chitin or chitosan suspension ranging in concentration from 0.1 to 10 mg/ml. In addition, PHL activity induced in rat granulation tissue by a NWF impregnated with a phosphate buffer solution was examined as a control. The PHL activity in each group remained low until 4 days post-implantation (Day 4). However, in the 10 mg chitin group, the PHL activity increased rapidly without scatter of the data at Day 7 and remained at a plateau until Day 14. In other groups, PHL activity increased linearly until Day 14. The data varied widely at Day 7. Compared to chitosan, chitin at the higher concentration was found to induce stable collagen synthesis in the early wound healing process. KEY WORDS: chitin, chitosan, prolyl hydroxylase.J. Vet. Med. Sci. 66(12): 1595-1598, 2004 Chitin and chitosan have been widely studied in both engineering and medicine. In the field of medicine, the effects of chitin and chitosan on wound healing have been the focus of great attention since Prudden et al. published a paper in 1970 stating that these agents accelerate wound healing [15]. Numerous reports describe the stimulatory effects of chitin and chitosan on tissue reactions involved in wound healing [1,[3][4][5][7][8][9][10][11][12][13]. Their most notable property is their propensity to induce abundant granulation tissue formation with angiogenesis. Previous reports indicated that chitin and chitosan induced fine collagen fibers histologically [3,11]. Moreover, chitin was found to enhance synthesis of types I, III, and IV collagen [4]. However, these reports indicate only the properties of the collagen synthesized. To understand more about wound healing acceleration by chitin and chitosan, it is important to clarify the quantity of collagen synthesized as well as its properties. In this paper, we evaluated the degree to which chitin and chitosan stimulate collagen synthesis by measuring prolyl hydroxylase activity, an activity necessary for collagen synthesis in rats [18].Thirty Wistar rats (adult females, weighing 270-300 g) were used in this study. The animals were purchased from Hamaguti Laboratory Co., Ltd. (Kobe, Japan). Chitin and chitosan were supplied by Sunfive Co., Ltd. (Tottori, Japan). Molecular weights and rates of deacetylation were determined using the viscoanetric method [19] and infra-red spectroscopic method [17], respectively. The data obtained for chitin and chitosan yielded molecular weights of 300 and 80 kD, and deacetylation rates of <10 and >80%, respectively. Chitin and chitosan having a mean particle size of 3.5 µm were sterilized separately using ethylene oxide gas and suspended in phosphate buffered solution (PBS, pH 7.2) at a concentration of 10 mg/ml. According to our measurements in a previous investigation [5], the optimum doses of chitin and chitosan in granulation tissue formation in rats w...

show abstract

“…Chitosan is inert, hydrophilic, biocompatible and biodegradable [4][5][6][7]. Its biodegradation leads to the release of aminosugars which can be incorporated into the metabolic pathways for glycosaminoglycans and glycoproteins, or excreted [8].…”

mentioning

confidence: 99%

Physico-chemical properties of Chitosan films

et al. 2004

View full text Add to dashboard Cite

Chitosan films obtained by dry phase inversion were prepared from an aqueous solution of chitosan in acetic acid. The films, of thickness less than 20 µm, were transparent, very flexible and had smooth surfaces. Increasing the film thickness induces an increase of the internal tensions and the consequent formation of a rough surface. Structural investigations by X-ray diffraction and Fourier transform IR analysis, showed that the chitosan films, as prepared, are amorphous. Further annealing to evaporate acetic acid and water traces, changed the amorphous phase into a more ordered phase, characterized by diffraction peaks at 2θ values of 9, 17, 20 and 23 degrees. Thermal investigations by TG, DTG, and DTA revealed that the decomposition of the chitosan films as prepared proceeds in two stages, starting from 180 • C and 540 • C.

show abstract

The discovery of a potent pure chemical wound-healing accelerator

Cited by 170 publications

References 10 publications

Chitosan as a starting material for wound healing applications

Chitosan as a starting material for wound healing applications

Effects of Chitin and Chitosan on Collagen Synthesis in Wound Healing

Physico-chemical properties of Chitosan films

Contact Info

Product

Resources

About