The dissolution of single crystals of hydroxyapatite in citric and lactic acids

Jongebloed, W.L.; Berg, P.J. Van Den; Arends, J

doi:10.1007/bf02059038

Cited by 57 publications

(24 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, some thin enamel crystals exhibited in these large cytoplasmic vacuoles showed central perforations and lateral defects resulting from different phases of progressive crystal dissolution. These dissolution patterns of enamel crystals resembled the patterns of human enamel crystals in the initial stages of carious dissolution or crystals that have been demineralized artificially by acids, as reported by many authors (Johnson, 1967;Jongebloed, 1974;Scott et al, 1974;Bres, et al, 1884;Tohoda et al, 1987). These results clearly demonstrate that odontoclasts that resorbed enamel demineralized the enamel matrix facing the ruffled border by secreting acids and that some liberated crystals from the partially demineralized enamel matrix were endocytosed via cytoplasmic vacuoles adjacent to the ruffled border and subsequently dissolved in their large cytoplasmic vacuoles, probably secondary lysosomes.…”

Section: Discussionsupporting

confidence: 50%

Ultrastructural features of odontoclasts that resorb enamel in human deciduous teeth prior to shedding

et al. 1998

View full text Add to dashboard Cite

Three dental hard tissues, i.e., cementum, dentin, and enamel, are resorbed by multinucleated cells referred to as "odontoclasts." These cells have morphological and functional characteristics similar to those of bone-resorbing osteoclasts. However, concerning enamel resorption, which is a process that may occur during tooth eruption, satisfactory ultrastructural data on odontoclastic resorption are still lacking. Ultrastructural and histochemical characteristics of odontoclasts resorbing enamel of human deciduous teeth prior to shedding were examined by means of light microscopy and transmission and scanning electron microscopy. Odontoclasts that that resorbed enamel were tartrate-resistant acid phosphatase (TRAP)-positive multinucleated giant cells that were essentially the same as those that resorbed dentin and cementum. Ultrastructurally, they had numerous mitochondria, lysosomes, and free polysomes in their cytoplasm. In addition, they were characteristically rich in large cytoplasmic vacuoles containing enamel crystals in the cytoplasm opposite the ruffled border. Although they extended a well-developed, ruffled border against enamel surface, a clear zone--an area typically devoid of organelles--was rarely seen in these cells. In many cases, the cells were in very close contact with the enamel surface by the peripheral part of their cytoplasm. The enamel prisms at the resorption surface contained more loosely packed and electron-lucent enamel crystals compared with those of unresorbed, intact enamel. Furthermore, numerous thin needle- or plate-like enamel crystals that were liberated from the enamel matrix were found in the extracellular channels of the ruffled border and in various-sized cytoplasmic vacuoles in their cytoplasm. The superficial layer of the enamel matrix undergoing odontoclastic resorption stained positively with toluidine blue and for TRAP activity. The results of the present study suggest that odontoclasts resorbing enamel secrete acids as well as organic components, including hydrolytic enzymes, into the resorption zone underlying their ruffled border and that they phagocytose crystals that have been liberated from the partially demineralized enamel matrix by acids, subsequently dissolving them intracellularly.

show abstract

Section: Discussionsupporting

confidence: 50%

Ultrastructural features of odontoclasts that resorb enamel in human deciduous teeth prior to shedding

et al. 1998

View full text Add to dashboard Cite

show abstract

“…The first is that the dislocation behavior under acid etching conditions is different whether it is a screw dislocation located at the crystal base [Jongebloed et al, 1974;Arends and Jongebloed, 1977] or the im print of edge dislocations on the crystal faces. It must, however, be noted that a screw dislocation, which is responsible for the helicoidal crystal growth, is always per fectly centered, whereas the dissolution ob served at the crystal base is always dis placed with respect to the center, which makes the first hypothesis rather unlikely.…”

Section: Discussionmentioning

confidence: 99%

Mechanisms of Acid Dissolution of Biological and Synthetic Apatite Crystals at the Lattice Pattern Level

Daculsi¹,

Kerébel²,

Kerebel³

1979

Caries Res

View full text Add to dashboard Cite

High resolution TEM made it possible to study acid dissolution of biological and synthetic apatite crystals at the lattice pattern level. It was shown that acid dissolution starts at the dislocation level and proceeds more frequently according to lattice families with large d spacings, i.e., (100), (110), (101) and equivalents. Acid dissolution is preferential according to the c axis, due to the existence of dislocations and probably to variations occurring in the composition and organization of the solid phase. It was hypothesized that Mg ions could help organizing a nucleation phase remaining in the central part of mature crystallites and that incorporation of fluoride ions to the lattice, related to dislocations, could stop diffusion process and progress of dissolution.

show abstract

“…Such fracturing would not necessarily affect the density of possible growth sites since the lattice defects responsible for these sites (such as screw and line dislocations) are thought to extend along a considerable portion if not the entire length of the crystals [22]. The observed concentration of new growth on these fractured surfaces may explain why the two enamel preparations were equally effective initially as seed materials, even though the guanidine HCI extracted preparation still contained small amounts of proteinaceous matrix material [15].…”

Section: Discussionmentioning

confidence: 99%

Transmission electron microscopic study of calcium phosphate formation in supersaturated solutions seeded with apatite

Doi

Eanes

1984

Calcif Tissue Int

View full text Add to dashboard Cite

The present study examined crystal growth on enamel and synthetic apatite seed surfaces in dilute supersaturated solutions by means of transmission electron microscopy. At all supersaturations, new growth initially appeared on the ends of the seed crystal. In solutions undersaturated with respect to octacalcium phosphate (OCP), this growth was needlelike in appearance. Above the solubility point for OCP, the growth frequently took the form of thin, platelike crystals. The relevance of these findings to precursor phase formation is discussed.

show abstract

The dissolution of single crystals of hydroxyapatite in citric and lactic acids

Cited by 57 publications

References 16 publications

Ultrastructural features of odontoclasts that resorb enamel in human deciduous teeth prior to shedding

Ultrastructural features of odontoclasts that resorb enamel in human deciduous teeth prior to shedding

Mechanisms of Acid Dissolution of Biological and Synthetic Apatite Crystals at the Lattice Pattern Level

Transmission electron microscopic study of calcium phosphate formation in supersaturated solutions seeded with apatite

Contact Info

Product

Resources

About