The distinguishing effects of low-intensity electromagnetic radiation of different extremely high frequencies on <i>Enterococcus hirae</i>
: growth rate inhibition and scanning electron microscopy analysis

Hovnanyan, Karlen; Kalantaryan, V. P.; Trchоunian, Armen

doi:10.1111/lam.12764

Cited by 7 publications

(7 citation statements)

References 25 publications

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“…The bacterial suspension was irradiated by EMI generator; model G4‐141 (State Scientific‐Production Enterprise ‘Istok’, Fryazino, Moscow Region, Russia) which emits (through conical antenna) coherent in time electromagnetic waves with the frequencies of 51·8 GHz and 53 GHz with 1 Hz frequency of amplitude modulation (frequency stability was 0·05%); the flux capacity was 0·06 mW cm −2 , as described before (Soghomonyan and Trchounian ; Hovnanyan et al . ). EMI with this amount of flux capacity has no effect on the temperature of bacterial suspension during irradiation: the change in temperature of suspension is below 0·1°C during the exposure.…”

Section: Methodsmentioning

confidence: 97%

“…These frequencies are of interest due to numerous results with lactobacilli and other bacteria reported before (Soghomonyan and Trchounian , ; Hovnanyan et al . ) and that these frequencies are suggested to be resonant for water molecules and might change water properties facilitating effects of EMI on bacteria (Torgomyan and Trchounian ; Soghomonyan et al . ).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, it has been shown that EMI of these frequencies have changed shape and sizes of bacteria, and the effects were distinguished between 51·8 GHz and 53 GHz (Hovnanyan et al . ) suggesting some different mechanisms of action. The cellular targets for EMI are water molecules, plasma membrane with the N,N’ ‐dicyclohexylcarbodiimide (DCCD)‐sensitive F O F 1 ‐ATPase and genetic material (Soghomonyan et al .…”

Section: Introductionmentioning

confidence: 99%

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The survival of irradiated lactobacilli in the simulated gastrointestinal conditions with antibiotic ceftazidime

Soghomonyan

Trchоunian

2018

Lett Appl Microbiol

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Significance and Impact of the Study: The probiotic property of lactobacilli is defined with survival in different conditions of human digestive tract even in the presence of antibiotics and subjected to electromagnetic irradiation (EMI) at the extremely high frequency. Despite the fact that EMI and antibiotic ceftazidime affected Lactobacillus acidophilus; the viable number of bacterial cells was decreased in in vitro gastrointestinal model, but they could to grow in fresh growth medium. The changes in the F O F 1 -ATPase activity were obtained at acidic pH. Thus, these bacteria can overcome acid barrier due to the F O F 1 -ATPase: the irradiation stimulates the F O F 1 -ATPase activity in the acidic conditions, but enhances the effects of ceftazidime. The results are important for identifying the mechanisms of lactobacilli survival for physical and chemical factors and valuable for use. AbstractLactobacillus acidophilus is one of the widespread probiotic bacteria that can overcome acid and bile barrier of stomach and intestine, respectively, and then have beneficial effects on the host improving its intestinal microbial balance. The cell membrane F O F 1 -ATPase is an important factor in the response and tolerance to low pH through the action of controlling the H + concentration between the cell cytoplasm and external medium. In this study, the effects of extremely high-frequency EMI at the frequencies of 51Á8 GHz and 53 GHz and cetfazidime ( lmol l À1 ) on survival of L. acidophilus VKM B-1660 in the gastrointestinal model in vitro and on ATPase activity of their membrane vesicles were investigated. Irradiated L. acidophilus survived in media with acid pH; the irradiation stimulated N,N'-dicyclohexylcarbodiimide-sensitive F O F 1 -ATPase activity under acidic conditions, but enhanced the inhibitory effects of ceftazidime. Probably irradiated L. acidophilus is overcoming the acid barrier even in the presence of ceftazidime due to the F O F 1 -ATPase. The obtained results can allow the use of L. acidophilus in food industry, veterinary and medicine.

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Section: Methodsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The survival of irradiated lactobacilli in the simulated gastrointestinal conditions with antibiotic ceftazidime

Soghomonyan

Trchоunian

2018

Lett Appl Microbiol

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“…The authors suggested this could be due to the MMW activation of bacterial DNA repair mechanisms. Other studies by an Armenian research group reported a reduction in E. coli cell growth when exposed to MMWs [39][40][41][42][43][44][45]. These studies reported that when E.coli cultures were exposed to MMWs in the presence of antibiotics, there was a greater reduction in the bacterial growth rate and an increase in the time between bacterial cell division compared with antibiotics exposure alone.…”

Section: Cell Proliferationmentioning

confidence: 94%

5G mobile networks and health—a state-of-the-science review of the research into low-level RF fields above 6 GHz

Karipidis¹,

Mate²,

Urban³

et al. 2021

J Expo Sci Environ Epidemiol

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The increased use of radiofrequency (RF) fields above 6 GHz, particularly for the 5 G mobile phone network, has given rise to public concern about any possible adverse effects to human health. Public exposure to RF fields from 5 G and other sources is below the human exposure limits specified by the International Commission on Non-Ionizing Radiation Protection (ICNIRP). This state-of-the science review examined the research into the biological and health effects of RF fields above 6 GHz at exposure levels below the ICNIRP occupational limits. The review included 107 experimental studies that investigated various bioeffects including genotoxicity, cell proliferation, gene expression, cell signalling, membrane function and other effects. Reported bioeffects were generally not independently replicated and the majority of the studies employed low quality methods of exposure assessment and control. Effects due to heating from high RF energy deposition cannot be excluded from many of the results. The review also included 31 epidemiological studies that investigated exposure to radar, which uses RF fields above 6 GHz similar to 5 G. The epidemiological studies showed little evidence of health effects including cancer at different sites, effects on reproduction and other diseases. This review showed no confirmed evidence that low-level RF fields above 6 GHz such as those used by the 5 G network are hazardous to human health. Future experimental studies should improve the experimental design with particular attention to dosimetry and temperature control. Future epidemiological studies should continue to monitor long-term health effects in the population related to wireless telecommunications.

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“…It has also been demonstrated that an electromagnetic wave irradiation for 5 s at 500-1000 kHz has bactericidal activity against several oral bacterial pathogens including E. faecalis [32]. In addition, several studies of extremely high frequencies EMF effect on Enterococcus hirae resulted in a decreased specific growth rate, but suggesting the differences in mechanisms of action [20,30,11].…”

Section: Effect Of the Exposure Time And The Conductivity Of The Medimentioning

confidence: 99%

The Influence of Electromagnetic Field on Viability of Marine Microalgae Tetraselmis Suecica and Bacteria Escherichia Coli and Enterococcus Faecalis

Brailo¹,

Pećarević²,

Grilec³

et al. 2018

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The objective of this study was to determine whether the use of the electromagnetic field (EMF) of 50 Hz frequency and magnetic induction of 0.25 T could make for successful inactivation of a phytoplankton species, namely, marine microalgae Tetraselmis suecica and two bacteria strains; Escherichia coli and Enterococcus faecalis. A number of laboratory electromagnetic field tolerance experiments with the selected organisms was performed; each microorganism was treated for various lengths of time; 1, 5 and 10 minutes, and in three various media with special regards to the conductivity. Bacteria were exposed to high, moderate and extremely low conductivity media, and the microalgae to high, low and extremely low conductivity media. The microbial viability was checked by counting the bacterial colony forming units, as well as alive and dead stained microalgae cells. It was found that the time of exposure to the EMF had a profound effect on the viability of T. suecica only in the extremely low conductivity media, and that it did not affect the viability of E. coli or E. faecalis at all. Sažetak Cilj ovoga istraživanja bio je utvrditi može li elektromagnetsko polje (EMP) frekvencije 50 Hz i magnetske indukcije od 0,25 T uspješno inaktivirati jednu morsku fitoplanktonsku vrstu; mikroalgu Tetraselmis suecica te dvije vrste bakterija; Escherichia coli i Enterococcus faecalis. Provedeni su laboratorijski pokusi tolerancije odabranih organizama na elektromagnetsko polje. Svaki mikroorganizam tretiran je u različitim razdobljima; 1, 5 i 10 minuta, te u tri različita medija s obzirom na vodljivost. Bakterije su bile izložene u mediju visoke, umjerene i ekstremno niske vodljivosti, a mikroalge u mediju visoke, niske i ekstremno niske vodljivosti. Mikrobna vijabilnost je ispitivana uz pomoć brojanja izraslih kolonija bakterija, te živih i mrtvih obojenih stanica mikroalgi. Utvrđeno je da je vrijeme izlaganja elektromagnetskom polju imalo velik utjecaj na vijabilnost T. suecica samo u mediju ekstremno niske vodljivosti i da nije uopće utjecalo na vijabilnost E. coli ili E. faecalis.

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The distinguishing effects of low-intensity electromagnetic radiation of different extremely high frequencies on Enterococcus hirae : growth rate inhibition and scanning electron microscopy analysis

Cited by 7 publications

References 25 publications

The survival of irradiated lactobacilli in the simulated gastrointestinal conditions with antibiotic ceftazidime

The survival of irradiated lactobacilli in the simulated gastrointestinal conditions with antibiotic ceftazidime

5G mobile networks and health—a state-of-the-science review of the research into low-level RF fields above 6 GHz

The Influence of Electromagnetic Field on Viability of Marine Microalgae Tetraselmis Suecica and Bacteria Escherichia Coli and Enterococcus Faecalis

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