The Distribution of Intestinal Parasites in Patients Presenting to a University Hospital in Istanbul: A Seven-year Retrospective Analysis

Polat, Erdal; Özdemir, Sinem; Sirekbasan, Serhat

doi:10.4274/tpd.galenos.2020.6653

Cited by 12 publications

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“…Thus, the importance of cryptosporidiosis and giardiasis is increased. [11][12][13] Infections caused by Cryptosporidium spp. and G. intestinalis are thought to constitute two of the first three pathogens among the most common protozoan infections that cause diarrhea in humans in the world, especially in developing countries.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Frequency of Cryptosporidium spp. and Giardia intestinalis in Patients with Suppressed Immune System

Bayraktar

CÖMERT>

Öztürk³

2022

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi

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Amaç: Giardia intestinalis ve Cryptosporidium spp. türleri gelişmiş ve gelişmekte olan ülkelerde ishale neden olan bağırsak parazitleridir. Özelikle bağışıklık sistemi baskılanmış hastalarda ve çocuklarda tedavi edilemediğinde ölümcül olabilmektedir. Bu çalışmada Harran Üniversitesi Araştırma ve Uygulama Hastanesine başvuran bağışıklık sistemi baskılanmış hastaların dışkı örneklerinde Cryptosporidium spp. ve G.intestinalis sıklığının araştırılması amaçlanmıştır. Yöntem: Bu çalışma Ocak 2017 ve Ocak 2019 yılları arasında hastanemize başvuran bağışıklık sistemi baskılanmış ve ishal şikâyeti olan toplam 100 hasta ile gerçekleştirildi. Hastaların dışkı örneklerinde immünokromatografik (İK) metot ile Cryptosporidium spp. ve G. intestinalis antijenleri arandı ve nativ-lugol ve kinyoun asit fast boyama ile ookist, kist-trofozoit açısından incelendi. Bulgular: Toplam sekiz (%8) hastada G. intestinalis saptandı. Bunlardan yedisi (%14) çocuk hasta (n=50) ve biri (%2) yetişkin (n=50) bir kadın hasta olduğu tespit edildi. Hastaların 3 (%3)’ünde ise Cryptosporidium spp. görüldü. Bunlardan ikisi (%4) çocuk ve biri (%2) yetişkin kadın hastalar idi. Sonuçlarımız hem Cryptosporidium hem de G. intestinalis’in bağışıklık sistemi baskılanmış çocuk hastalarda (sırasıyla %4 ve %14) daha fazla saptandığını göstermiştir. Sonuç: Dışkı örneklerinde antijen saptayan İK metodun maliyeti yüksek olmasına rağmen çalışması kolay, erken sonuç vermesi ve etkensel tanı yöntemlerindeki zorluklara yardımcı olduğu görülmektedir. Sınırlı sayıda araştırılmış örnek bulunmasına rağmen bulgularımız bu iki parazitten kaynaklı enfeksiyonların Türkiye'de veya Avrupa ülkelerinde yapılan diğer çalışmalardaki görülme sıklığından daha yüksek olduğunu gösterdi.

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Section: Discussionmentioning

confidence: 99%

Investigation of the Frequency of Cryptosporidium spp. and Giardia intestinalis in Patients with Suppressed Immune System

Bayraktar

CÖMERT>

Öztürk³

2022

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi

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“…The diseaes caused by these parasites are cosmopolitan diseases and the prevalence of the diseases varies according to the geographical region, the population studied, the sample selection technique and the diagnostic methods used [5][6][7] . The diagnosis of these diseases can be based directly on the presence of parasites spores in stool samples or indirectly by serological methods.…”

Section: Introductionmentioning

confidence: 99%

Identification of Spore-Forming Intestinal Parasites with Pentaplex Real-Time PCR

Eroglu

2023

Dicle Tıp Dergisi

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Objective: Spore-forming intestinal parasites; Cryptosporidium spp., Cyclospora spp., Cytoisospora spp., Encephalitozoon spp., and Sarcoystis spp. are very common in immunocompromised patients, but these parasites are overlooked by healthcare proffesionals. It was aimed to develop a new pentaplex real-time PCR panel for the identification of spore-forming intestinal parasites in this study. Methods: Primer-probes for pentaplex real-time PCR were designed using the “PrimerQuest Tool (Integrated DNA technologies, Coralville, USA) software program” and “Multiple sequence alignment use a computer software Primer Express™Software v3.0.1 Lience (ThermoFisher Scientific, Waltham, USA)”. The primer-probes designed in the study were spore-forming Cryptosporidium spp. (ATCC®87715™), Cyclospora spp. (ATCC®PRA-3000SD™), Cystoispora spp. (KF648871), Encephalitozoon spp. (FJ026010) and Sarcocystis spp. (ATCC®CCL-70) parasites were amplified with DNA isolates from the American Type Culture Collection (ATCC) and then these primer-probes were validated with 232 DNA samples obtained from the stools of the patient samples. Results: It was found that Cycle Threshold (Ct) ±25.7, Standard curve (R2 ): ±0,993, and Efficiency (E): %96,1 according to the results of multiplex real-time PCR analysis. Similar results were found in pentaplex real-time PCR analysis of DNA isolates of stool samples. When the pentaplex real-time PCR results of DNA samples isolates from stool samples were compared with the positivie predictive value results of traditional methods, it was found that the pentaplex results were higher. Conclusion: The new designed pentaplex real-time PCR panel can be used in the diagnosis of spore-forming intestinal parasites, which are very common in immunocompromised patients. Thus, the diagnosis of five different parasites can be made faster, more economically and faster with a single reaction.

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“…Many studies have been conducted to determine the prevalence of intestinal parasites in various university hospitals in our country. Parasite detection rate, which was 6.4% in patients who applied to Dokuz Eylül University Faculty of Medicine, 5.1% in Süleyman Demirel University Faculty of Medicine, 3.6% in Uludağ University Faculty of Medicine, 14.9% in Gazi University Faculty of Medicine, 10.5% in Cumhuriyet University Faculty of Medicine, 15.7% in Erciyes University Faculty of Medicine, and 1.8% in Ondokuz Mayıs University Faculty of Medicine(6,7). In our study and İnönü University, this rate was determined as 5.52%.Macroscopic and microscopic parasitic analysis of a total of 16083 stool examinations sent to Konya Selcuk University Faculty of Medicine Microbiology and Clinical Microbiology laboratory for coproparasitological examination between 1992-1998 was performed, and parasites were detected in 1009 (6.27%) of them.…”

mentioning

confidence: 99%

Distribution of intestinal parasites in patients applied to Inonu University Turgut Ozal Medical Center Parasitology Laboratory between 2018-2022

YAR¹,

Aksoy²,

ÇİÇEK³

et al. 2022

targetmedj

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Parasitic diseases are seen as an important public health problem, especially in developing countries and affect approximately four billion people in the world (1). Various factors such as gender, age, socioeconomic level, personal hygiene habits, education level, nutrition, staying in communal places, traditions, eating habits, infrastructure and seasonal changes are effective on these incidence rates (2).

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The Distribution of Intestinal Parasites in Patients Presenting to a University Hospital in Istanbul: A Seven-year Retrospective Analysis

Cited by 12 publications

References 0 publications

Investigation of the Frequency of Cryptosporidium spp. and Giardia intestinalis in Patients with Suppressed Immune System

Investigation of the Frequency of Cryptosporidium spp. and Giardia intestinalis in Patients with Suppressed Immune System

Identification of Spore-Forming Intestinal Parasites with Pentaplex Real-Time PCR

Distribution of intestinal parasites in patients applied to Inonu University Turgut Ozal Medical Center Parasitology Laboratory between 2018-2022

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