The distribution of plasmids among a representative collection of Scottish strains of salmonellae

Platt, D. J.; Brown, D. J. F.; Munro, Donald

doi:10.1017/s002217240006527x

Cited by 17 publications

(8 citation statements)

References 14 publications

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“…berta contained 1.8 plasmids per strain. In comparison, Platt et al (14) demonstrated plasmids in 92% of the S. typhimurium strains investigated (mean 1.9, range 0-7 plasmids per strain). Plasmids were more common in S .…”

Section: Discussionmentioning

confidence: 87%

Plasmid profiles of Salmonella enterica serovar berta isolated from broilers in Denmark

SØRensen¹,

Brown²,

Bisgaard³

et al. 1991

APMIS

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Salmonella enterica serovar berta was isolated for the first time in Denmark in October 1984. This serovar has subsequently become endemic in the broiler industry. Plasmid profile analysis of 610 strains of S. berta isolated from broilers and broiler parent stock in the period October 1984 to July 1989 showed that plasmids were present in 85.7% of strains investigated, with a mean of 1.8 plasmids per strain. A significant increase in the mean number of plasmids per strain was seen during the observation period. Seventeen different plasmid sizes and 90 different plasmid profiles were identified. Plasmid profiling of S. berta appears to represent a promising tool for analysing the epidemiology of S. berta infections in broilers.

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Section: Discussionmentioning

confidence: 87%

Plasmid profiles of Salmonella enterica serovar berta isolated from broilers in Denmark

SØRensen¹,

Brown²,

Bisgaard³

et al. 1991

APMIS

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“…The presence of plasmids in selected Salmonella strains was analysed according to the alkaline lysis method of Platt et al. (1986) after overnight incubation of cells with shaking in brain heart infusion (BHI) broth at 37°C.…”

Section: Methodsmentioning

confidence: 99%

A rapid monitoring assay for the detection of Salmonella spp. and Salmonella Senftenberg strain W775 in composts

Clercq

Ceustermans

Heyndrickx

et al. 2007

Journal of Applied Microbiology

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Aims: The composting process needs to be validated for its hygienic status in order to ensure that it is free of pathogens. Generally, this is evaluated through temperature monitoring, or additionally through active inoculation and monitoring of indicator organisms. We aimed to develop a monitoring method for the heat‐resistant indicator organism Salmonella enterica ssp. enterica serovar Senftenberg strain W775 for detection in composting biowastes. Methods and Results: The method development is comprised of: (i) optimization of molecular detection of bacteria belonging to the genus Salmonella; (ii) identification of a DNA marker for Salmonella strain W775; and (iii) development of a multiplex polymerase chain reaction (PCR)‐based on both DNA markers. Subsequently, Salmonella strain W775 was inoculated and monitored during composting of biowastes in an industrial composting facility. Conclusions: A highly sensitive and specific detection of viable cells was obtained by enriching the compost sample prior to multiplex PCR analysis. Complete inactivation of Salmonella strain W775 was obtained within 4 days in an industrial composting facility at temperatures ranging between 41 and 57°C. Significance and Impact of the Study: We describe a monitoring method for the simultaneous detection of naturally occurring Salmonella strains and artificially introduced Salmonella strain W775 in composting biowastes that can be applied in routine analysis.

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“…As part of a survey of salmonella plasmids in 1985 strains of Dublin were all found to harbour the serotype-specific plasmid (SSP) alone [14]. This contrasts with the situation in both Typhimurium and Enteritidis where a small but significant proportion of strains was plasmid free [9].…”

Section: Introductionmentioning

confidence: 99%

Diversity and molecular variation among plasmids inSalmonella entericaserotype Dublin based on restriction enzyme fragmentation pattern analysis

1995

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SUMMARYMolecular variation within and between plasmids ofSalmonella enterica serotype Dublin was analysed. Such variation has been demonstrated in the serotypespecific plasmids (SSP's) of Typhimurium and Enteritidis. The two aims of this study were to determine the plasmid diversity in a host-adapted serotype and also the incidence of molecular variation in the SSP among strains of Dublin using restriction endonuclease fragmentation pattern (REFP) analysis with Pstl, Smal and EcoRV. Sixty-five strains were examined from seven countries. Plasmid profile and REFP analysis showed that none of the strains was plasmid-free. Seventy-seven percent of the strains possessed the 72 kb SSP either alone or in combination with another plasmid; 23 % harboured plasmids which were molecular variants of the SSP. Four of the variants were more closely related to each other than to the reference SSP and were harboured by Dublin isolated from both the USA and Europe. A further three were shown to be cointegrate plasmids and were similarly distributed. Thirty-two percent of strains possessed the SSP alone. None of the UK strains was resistant to any of the antimicrobial agents tested whereas 74 % of the remaining strains were resistant to between one and five antimicrobial agents. This study corroborates previous findings concerning the high degree of stability of the SSP and confirmed the clonal nature of Dublin.Co-resident plasmids provided evidence of sub-clones within localized geographical areas.

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The distribution of plasmids among a representative collection of Scottish strains of salmonellae

Cited by 17 publications

References 14 publications

Plasmid profiles of Salmonella enterica serovar berta isolated from broilers in Denmark

Plasmid profiles of Salmonella enterica serovar berta isolated from broilers in Denmark

A rapid monitoring assay for the detection of Salmonella spp. and Salmonella Senftenberg strain W775 in composts

Diversity and molecular variation among plasmids inSalmonella entericaserotype Dublin based on restriction enzyme fragmentation pattern analysis

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