The distribution of radioactivity in brains of rats given [N-methyl-11C]PK 11195 in vivo after induction of a cortical ischaemic lesion

Neuroinflammation Extends Brain Tissue at Risk to Vital Peri-Infarct Tissue: A Double Tracer [11C]PK11195- and [18F]FDG-PET Study

“…Several earlier studies in animals and in humans analyzed the localization and time course of strokeinduced neuroinflammation, and found inflammation predominantly within the ischemic core (Cremer et al, 1992;Garcia et al, 1994) or in peri-infarct regions (Gerhard et al, 2000;Pappata et al, 2000;Mabuchi et al, 2000;Price et al, 2006). As discussed, we attribute differences in localization to different experimental ischemia models or to the individual clinical situation, respectively.…”

“…11 C-labeled PK11195 enables in vivo imaging of the PBR status using positron emission tomography (PET). At variance with the above-mentioned autoradiographic study in human stroke, PET studies in human and animal strokes showed heterogeneous results regarding the localization and time course of [ 11 C]PK11195 uptake, with binding occurring within the ischemic core (Cremer et al, 1992), the periinfarct region (Gerhard et al, 2005;Price et al, 2006), or remote areas (Pappata et al, 2000) at varying time points ranging from 3 to 30 days after ischemia. In a recent study using transient occlusion/reperfusion of the middle cerebral artery (MCA) in rats, [ 11 C]PK11195 was observed to bind to the core of the ischemic territory (Rojas et al, 2007), with a maximum at 4 to 7 days after focal ischemia.…”

PET Imaging of Disease Progression and Treatment Effects in the Experimental Autoimmune Encephalomyelitis Rat Model

“…For each PET scan, animals were anesthetized with isoflurane in medical air (5% for induction, 1.0%-2.0% for maintenance). For imaging of microglia activation or monocyte invasion, 30-60 MBq of 11 C-PK11195 (25) were injected into the tail vein. After 40 min, the animals were positioned in the PET camera with the head and upper part of the spinal cord (cervical plus half of the thoracic portion) in the field of view.…”

The 18-kDa Mitochondrial Translocator Protein in Human Gliomas: An 11C-(R)PK11195 PET Imaging and Neuropathology Study

“…11 C-(R)PK11195 was synthesized according to established methods (16,17). PET scans were obtained on the High Resolution Research Tomograph (Siemens/CTI) as described previously (15). The injected dose of 11 C-(R)PK11195 was 509 6 123 MBq, with a specific radioactivity of 132 6 49GBq/mmol.…”

“…In addition, most second-generation TSPO ligands have been used only in experimental animals and are not fully characterized in humans (12)(13)(14). In our previous study, we have demonstrated that 11 C-(R)PK11195 is a suitable radiotracer for glioma imaging (15). In the present study, we hypothesized that TSPO expression in gliomas differs between tumor grades and histotypes, which could be detected by 11 C-(R)PK11195 PET imaging; this neuroimaging modality has the potential to identify transforming LGGs and be used to stratify patients for TSPOtargeted treatment that would challenge neoplastic cells.…”