A monthlong, high-resolution buoy time series from the surface ocean of the Changjiang River plume in early autumn 2013 (30-min sampling frequency) shows great variability in the partial pressure of carbon dioxide (pCO 2 ) and other physical and biogeochemical parameters. Early in the deployment, surface pCO 2 decreased by~117 μatm in a single day (11-12 September, from an initial value of~527 μatm); a similar decline of 62 μatm occurred five days later (to~378 μatm). Both drawdown events were associated with strong vertical stratification, high chlorophyll a concentrations, and oxygen supersaturation. A one-dimensional mass balance model suggests that biological production was responsible for more than half the pCO 2 decrease observed during 10-23 September. Subsequently, in association with strong winds, the mixed layer rapidly deepened and surface pCO 2 increased sharply (by about 108 μatm in late September and again in early October). Vertical mixing accounted for more than half of this pCO 2 increase, which offset more than the earlier biologically driven CO 2 drawdown. In the presence of such strong temporal variations of pCO 2 , sampling frequency exerts a substantial influence on air-sea CO 2 flux calculations for the Changjiang River plume and similar coastal areas. Compared to daily sampling, even weekly sampling would result in a bias of up to ±4.7 mmol C · m À2 · day À1 or ±63% error.Plain Language Summary Determining the mechanisms that control sea surface pCO 2 and its variability in coastal waters is an important step toward estimating global air-sea CO 2 fluxes and projecting future atmospheric CO 2 levels. In this study, a 31-day high-resolution buoy time series from the surface ocean of the Changjiang River plume in early autumn 2013 show great variability in the partial pressure of carbon dioxide (pCO 2 ) and other parameters. Early in the deployment, surface pCO 2 decreased sharply in short period (one to five days). The pCO 2 drawdown events were associated with strong vertical stratification, high chlorophyll a concentrations, and oxygen supersaturation. A mass balance model suggests that biological production was responsible for more than half the pCO 2 decrease observed. Subsequently, in association with strong northeast winds, the mixed layer rapidly deepened and surface pCO 2 increased sharply. Vertical mixing accounted for more than half of this pCO 2 increase. In the presence of such strong temporal variations of pCO 2 , sampling frequency exerts a substantial influence on air-sea CO 2 flux calculations for the Changjiang River plume and similar coastal areas.With respect to the partial pressure of carbon dioxide (pCO 2 ) at the sea surface, the biological processes triggered and maintained by subsurface and riverine nutrient inputs are particularly important in lowering surface pCO 2 values (Cao et al.