The Distribution of Some Hydrolytic Enzymes in the Semen of the Domestic Fowl, Gallus Domesticus

McIndoe, W. M.; Lake, P. E.

doi:10.1530/jrf.0.0400359

Cited by 17 publications

(1 citation statement)

References 28 publications

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“…The localization of different enzymes in the testes and accessory sex organs has mainly been studied in the male fowl (Lake, 1962;Angulo & Bosch, 1964;Woods & Domm, 1966;Bishop, 1967;Tingari & Lake, 1972;Tingari, 1973;Kugler, 1975) and partly in some other species: house sparrow (Somani & Guraya, 1971 ;Urry et al, 1975), pigeon (Blanco et al, 1964), Japanese quail (Yamada, 1972) and swift (Guraya, 1970). Some studies have been performed on the metabolism of ejaculated sperm of domesticated birds (Buckland, 1971a, b ;McIndoe & Lake, 1974;Lessley & Brown, 1975) and on the Japanese quail (Buxton & Orcutt, 1975). As the sexual activities in the Pied flycatcher, e.g.…”

Section: Introductionmentioning

confidence: 99%

Enzyme histochemical studies in testes and seminal vesicles of the Pied flycatcher (Ficedula hypoleuca) during the breeding season

Silverin

1978

Journal of Zoology

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Enzymatic conditions in testes and seminal vesicles of the Pied flycatcher during the breeding season were studied by histochemical techniques. The following enzyme activities were studied: glucose‐6‐phosphatase dehydrogenase (G‐6‐PDH), NADPH2‐diaphorase, glutamic dehydrogenase (GDH), NADH2‐diaphorase, adenosine triphosphatase (ATPase) and acid phosphatase. Phospholipids and PAS‐positive substances were also studied. Towards the end of the nest‐building period, a time when the males showed maximal sexual activities, some enzyme activity changes were observed. There was an increase of the GDH activity in the sperm bundles, an increase of the ATPase activity in the interstitium and tunica propria of the tubuli seminiferi. The phospholipid content increased in all spermatogenetic cells and the PAS‐positive material increased in the Sertoli cells and spermatozoa. The enzymic activities indicated a steroid production in the Leydig cells. The epithelium of the seminal vesicles did not show any variations in enzyme activities as long as the Leydig cells persisted. Most enzymes showed a very high activity during this time, indicating a secretory function of the epithelium. The amount of phospholipids increased at the end of the nest‐building period. Parallel with the degeneration of the epithelium the enzymic activities, as well as the phospholipid content, were lowered markedly.

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Section: Introductionmentioning

confidence: 99%

Enzyme histochemical studies in testes and seminal vesicles of the Pied flycatcher (Ficedula hypoleuca) during the breeding season

Silverin

1978

Journal of Zoology

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Arylsulphatase activity in seminal plasma from fowl semen

Droba¹,

Droba²

1981

J. Exp. Zool.

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Arylsulphatase from seminal plasma of fowl was characterized with a crude enzyme preparation using nitrocatechol sulphate as the substrate. Arylsulphatase activity was considerable and showed a shift of pH optimum dependent on the incubation time and on the presence of sulphate and chloride ions. The electrophoretic pattern of enzymic activity showed three separate bands. It is concluded that both arylsulphatases A and B may be present in seminal plasma.Among several enzymes of lysosomal origin found in fowl semen, arylsulphatase (EC 3.1.6.1) has not been detected thus far (McIndoe and Lake, '74). The present paper reports a considerable arylsulphatase activity in fowl seminal plasma. MATERIALS AND METHODSTwenty fowls (Gallus domesticus) of New Hampshire strain, aged 12 months, kept at the Test Station of Experimental Poultry, Zootechnical Institute, Chorzel6w, were used as the semen source. The animals were fed a commercial breeder's diet. Semen was collected according to Burrows and Quinn ('37).Semen collected from 20 fowls (approximately 10 ml) was centrifuged at 5,000 g for 120 min at 5°C. The obtained supernatant (seminal plasma containing approximately 7.5 mg protein per ml) was dialysed against distilled water (50-fold water volume excess with two water changes) and stored in the refrigerator. The enzymic activity of individual samples varied and usually showed a several-fold increase after the dialysis.Arylsulphatase activity of seminal plasma was determined according to Baum et al. ('58). Equal volumes of seminal plasma diluted eight times with distilled water) and 2 mN nitrocatechol sulphate (Sigma) in 0.5 M sodium acetate-acetic acid buffer with or without 1.7 M NaCl or 25 mM K2S04, adjusted to the appropriate pH (at room temperature) were mixed together and incubated at 37°C. Samples (0.5 ml) were withdrawn at suitable time intervals and added to 1.3 ml of 1 N NaOH.Optical density (OD) was read at 515 nm against appropriate blank sample using Specol photocolorimeter and cuvettes of 10-mm path length.Polyacrylamide gel electrophoresis at pH 8.9 was carried out according to Davis ('64) Bands with arylsulphatase activity were visualized by incubating the gels in 1 mM nitrocatechol sulphate in 0.25 M sodium acetateacetic acid buffer (pH 5.0) for 30 min at 37°C; gels were subsequently transferred to 1.0 N NaOH for 2-3 min and immediately photographed before the red bands had faded.Protein concentrations were determined according to Lowry et al. ('51). RESULTS AND DISCUSSIONSeminal plasma from fowl semen shows a marked arylsulphatase activity, the optimum pH of which is due to the incubation time and the presence of sulphate and chloride ions. In case of the reaction mixture containing substrate and seminal plasma in acetate buffer, there was a marked shift of pH optimum from pH 5.5 for 5-min incubation to pH 5.0 for 60 and 90 min incubation. If the reaction mixture contained additionally 12.5 mM KzS04, the enzyme activity was higher at more alkaline pH levels and again a time dependent shift of pH...

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Fertilization in Birds

Wishart

Horrocks

2000

Fertilization in Protozoa and Metazoan Animals

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The Distribution of Some Hydrolytic Enzymes in the Semen of the Domestic Fowl, Gallus Domesticus

Cited by 17 publications

References 28 publications

Enzyme histochemical studies in testes and seminal vesicles of the Pied flycatcher (Ficedula hypoleuca) during the breeding season

Enzyme histochemical studies in testes and seminal vesicles of the Pied flycatcher (Ficedula hypoleuca) during the breeding season

Arylsulphatase activity in seminal plasma from fowl semen

Fertilization in Birds

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