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A QUICKLY REACTING FORM OF HAEMOGLOBIN 303 reacting haemoglobin (Hb*) is formed. If pchloromercuribenzoate is added almost all the reduced haemoglobin formed is Hb*. At alkaline pH the chief product of photolysis is Hb* even in the absence of p-chloromercuribenzoate. 3. At alkaline pH Hb* reverts spontaneously to ordinary haemoglobin with a velocity constant of about 200 sec.-' at 10. 4. The second-order velocity constant for the combination of Hb* with carbon monoxide is 1-8 x 106M-1 sec.-' at 1°and the activation energy 5-6 kcal. 5. The bearing of these results on the use of flash photolysis in the study of haemoglobin kinetics is discussed. Thanks are due to Miss M. C. Sykes for technical assistance and to the Medical Research Council for help with the expenses of the work.

In part 3 of this series (Davidson & Smellie, 1952b) a method was described for the accurate determination of the specific activities of the individual ribonucleotides of the ribonucleic acid (RNA) of the tissues of animals which had received radioactive phosphorus. This method has now been applied to ,he RNA of cell nuclei and of the various morphoogical fractions of the cell cytoplasm. Preliminary aotes on this work have already been published (Davidson, McIndoe, & Smellie, 1951; Smellie & McIlndoe, 1952) and a full account is given here. METHODS

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