The Phosphorus Compounds of the Cell Nucleus

McIndoe, W. M.; Davidson, James

doi:10.1038/bjc.1952.23

Cited by 76 publications

(10 citation statements)

References 17 publications

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“…The very high specific activity of the ribonucleotides from nuclear RNA is consistent with the results of other authors whose work has recently been reviewed by McIndoe & Davidson (1952).…”

Section: Resultssupporting

confidence: 91%

“…t I.C.I. Research Fellow. method as described by McIndoe & Davidson (1952) was employed in either case. The cytoplasmic supernatant material from the sucrose suspension was routinely examined microscopically to verify the absence of nuclei.…”

mentioning

confidence: 99%

“…Nuclear RNA was obtained as a solution of mixed ribonucleotides by the modification of the Schmidt & Thannhauser (1945) procedure described by McIndoe & Davidson (1952). These were also separated by ionophoresis on paper.…”

mentioning

confidence: 99%

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Phosphorus compounds in the cell. 4. The incorporation of radioactive phosphorus into liver cell fractions

Smellie

McIndoe

Logan

et al. 1953

Biochemical Journal

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In part 3 of this series (Davidson & Smellie, 1952b) a method was described for the accurate determination of the specific activities of the individual ribonucleotides of the ribonucleic acid (RNA) of the tissues of animals which had received radioactive phosphorus. This method has now been applied to ,he RNA of cell nuclei and of the various morphoogical fractions of the cell cytoplasm. Preliminary aotes on this work have already been published (Davidson, McIndoe, & Smellie, 1951; Smellie & McIlndoe, 1952) and a full account is given here. METHODS

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“…The very high specific activity of the ribonucleotides from nuclear RNA is consistent with the results of other authors whose work has recently been reviewed by McIndoe & Davidson (1952).…”

Section: Resultssupporting

confidence: 91%

mentioning

confidence: 99%

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Phosphorus compounds in the cell. 4. The incorporation of radioactive phosphorus into liver cell fractions

Smellie

McIndoe

Logan

et al. 1953

Biochemical Journal

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“…None of the dietary conditions affected the average DNA content of the liver nuclei, although some of them produced marked changes in the chemical composition or histological appearance of the liver. Similar results for the effect of fasting have been reported by Mirsky & Ris (1951) and McIndoe & Davidson (1952), and for the effect ofprotein deficiency by Campbell & Kosterlitz (1952).…”

supporting

confidence: 88%

“…If the tumour cells have diploid nuclei the average DNA content/nucleus will fall in an organ like the adult rat liver which normally contains polyploid nuclei (Table 4). If the tumour nuclei contain more DNA than the nuclei of the normal tissue, as happens in at least one fowl tumour (McIndoe & Davidson, 1952), the average will be higher in the cancerous tissue. (2) If the tumour is growing at a fairly rapid rate it may contain a significant proportion of nuclei that are about to divide and have more than the normal resting complement of DNA.…”

mentioning

confidence: 99%

The deoxyribonucleic acid content of the rat cell nucleus and its use in expressing the results of tissue analysis, with particular reference to the composition of liver tissue

Thomson

Heagy

Hutchison

et al. 1953

Biochemical Journal

263

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I953 activity of diphosphopyridinenucleotidase is greatly increased (Nason et al. 1951)-there is no reason to attribute invariably the disorders of metabolism which occur in zinc deficiency to a primary impairment of protein synthesis. Copper deficiency has been claimed to have marked effects on the protein metabolism of green plants (Gilbert, Sell & Drosdoff, 1946; Wood & Womersley, 1946; Lucas, 1948; Gilbert, 1951), and so the observation that the aldolase activity in the leaves of copper-deficient oats remains unimpaired, suggests that the lowered aldolase activity in the leaves of zinc-deficient oats is not due primarily to a decreased protein synthesis; although zinc might be specifically involved in the synthesis of the enzyme itself. The third possibility that the aldolases of green leaves are activated by Zn2+ or by zinc-containing co-enzymes is being examined. SUMMARY Aldolase activity was found to be materially decreased in tissue suspensions prepared from the leaves of zinc-deficient plants of Trifolium subterrane,um and of Avena 8ativa, but was unchanged by copper deficiency. The author is grateful to K. Powrie for supplying some of the experimental material: to G. B. Jones for the polarographic estimation of zinc and copper and to V. Stephen who set up the aeration apparatus and is particularly indebted to H. R. Marston, F.R.S., Chief of the Division of Biochemistry and General Nutrition, C.S.I.R.O., for his advice and criticism during the experiment and his help in preparation of the manuscript. Glasshouse accommodation was made available by Prof. J. G. Wood of the Botany Department of the University of Adelaide, and this courtesy is gratefully acknowledged.

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New Developments in the Chemical Determination of Nucleic Acids

Webb

Levy

1958

Methods of Biochemical Analysis

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The Phosphorus Compounds of the Cell Nucleus

Cited by 76 publications

References 17 publications

Phosphorus compounds in the cell. 4. The incorporation of radioactive phosphorus into liver cell fractions

Phosphorus compounds in the cell. 4. The incorporation of radioactive phosphorus into liver cell fractions

The deoxyribonucleic acid content of the rat cell nucleus and its use in expressing the results of tissue analysis, with particular reference to the composition of liver tissue

New Developments in the Chemical Determination of Nucleic Acids

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