The glutamic acid and creatine content of cock seminal plasma

Lake, P. E.; McIndoe, W. M.

doi:10.1042/bj0710303

Cited by 31 publications

(11 citation statements)

References 13 publications

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“…Subsequently, no sperm motility was recorded in normal saline diluted group at 48 h of storage at low temperature. This may be due to the fact that normal saline is lack of energy source (sugar) and constituents like glutamic acid responsible for maintaining osmotic pressure of avian semen (Lake and McIndoe, 1959;Sexton and Fewlass, 1978). A significant reduction in sperm motility during 24/48 h storage was also recorded in rest of the three semen diluents (Table 1).…”

Section: Resultsmentioning

confidence: 83%

Selection of Diluent for Short Term Preservation of Guinea Fowl Semen

Mohan¹,

Sharma²,

Kolluri³

et al. 2015

Asian J. of Animal and Veterinary Advances

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This study was conducted to identify the suitable diluent for short-term preservation (0, 24 and 48 h) of Guinea fowl (pearl variety) semen. Semen samples were collected and diluted with normal saline, CARI, BPSE and Lake's semen diluents and kept at 6°C for storage. No significant difference was found in sperm motility among the different diluents at 0 h (freshly ejaculated) storage period. A significant (p<0.05) reduction was found in sperm motility among all the diluents at 24 h storage period. Subsequently, there was no sperm motility was recorded in normal saline diluted semen samples at 48 h storage period. While a reduction (p<0.05) in sperm motility was recorded in rest of the three semen diluents. The mean values for fertility with freshly ejaculated semen during 2-12 days of fertile period were recorded as 66.27±4.08, 78.90±3.08, 64.90±4.87 and 67.99±6.36% whereas, after 24 h storage of semen, fertility reduced drastically such as: 0, 41.97±7.27, 36.09±8.72 and 30.90±7.12% in normal saline, CARI, BPSE and Lake's semen diluent, respectively. No fertility was noticed at 48 h storage in any semen diluent. Irrespective of the storage period and diluents, CARI semen diluent expressed better fertility than others in Guinea fowl.

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Section: Resultsmentioning

confidence: 83%

Selection of Diluent for Short Term Preservation of Guinea Fowl Semen

Mohan¹,

Sharma²,

Kolluri³

et al. 2015

Asian J. of Animal and Veterinary Advances

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“…biochemical composition of chicken and turkey semen see review, Lake, 1995 . Glutamic acid, the most prominent anionic constituent of avian seminal plasma, became Ž . a standard component of diluents Lake and Mc Indoe, 1959 . There are many diluents available for poultry semen, both published recipes and commercially available products.…”

Section: Semen Diluentsmentioning

confidence: 99%

Storage of poultry semen

Donoghue

Wishart

2000

Animal Reproduction Science

190

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“…Glutamate is the principal free amino acid in the testis and semen of most mammalian species, where it occurs mainly in the seminal plasma rather than in the spermatozoa (see Mann, 1964, p. 97 Lake & Mclndoe (1959) and Ahluwalia & Graham (1966) for the origin and distribution of amino acids in cock semen. The present studies were designed to examine the amino acid composition of fluid collected directly from the testis of conscious rams (Voglmayr, Waites & Setchell, 1966) and to compare this with blood plasma, testicular lymph, epididymal plasma and seminal plasma from entire and vasectomized rams.…”

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confidence: 99%

Amino acids in ram testicular fluid and semen and their metabolism by spermatozoa

Setchell

Hinks

Voglmayr

et al. 1967

Biochemical Journal

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1. The testis of the ram secretes considerable amounts of amino acids (200μmoles/day) into the fluid collected from the efferent ducts. The principal amino acid in this testicular fluid is glutamate, which is present in concentrations about eight times those in testicular lymph or in blood from the internal spermatic vein. 2. The concentration of glutamate in seminal plasma from the tail of the epididymis is about ten times that in testicular fluid, and, though glutamate is the major amino acid in ejaculated seminal plasma, its concentration is less than in epididymal plasma. 3. After the intravenous infusion of [U−14C]glucose, labelled glutamate was found in the testicular fluid. Radioactivity was also detected in alanine, glycine, serine plus glutamine and aspartate. Alanine had the highest specific activity, about 50% of the specific activity of blood glucose. 4. When [U−14C]glutamate was infused, the specific activity of glutamate in testicular fluid was only about 2% that in the blood plasma. 5. Testicular and ejaculated ram spermatozoa oxidized both [U−14C]glutamate and [U−14C]leucine to a small extent, but neither substrate altered the respiration from endogenous levels. 6. No radioactivity was detected in testicular spermatozoal protein after incubation with [U−14C]glutamate or [U−14C]leucine. Small amounts of radioactivity were detected in protein from ejaculated ram spermatozoa after incubation with [U−14C]glutamate. 7. The carbon of [U−14C]glucose was incorporated into amino acids by testicular spermatozoa; most of the radioactivity occurred in glutamate.

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The glutamic acid and creatine content of cock seminal plasma

Cited by 31 publications

References 13 publications

Selection of Diluent for Short Term Preservation of Guinea Fowl Semen

Selection of Diluent for Short Term Preservation of Guinea Fowl Semen

Storage of poultry semen

Amino acids in ram testicular fluid and semen and their metabolism by spermatozoa

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