The distribution of vesicular acetylcholine transporter in the human male genitourinary organs and its co-localization with neuropeptide Y and nitric oxide synthase

Dixon, J. S.; Jen, Phillip Y.P.; Gosling, J. A.

doi:10.1002/(sici)1520-6777(2000)19:2<185::aid-nau9>3.0.co;2-2

Cited by 68 publications

(60 citation statements)

References 21 publications

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“…The present results dealing with adrenergic and cholinergic innervation pattern of urinary bladder wall Burnstock 1989, Persson et al 1995) and in other mammalian species studied, including humans (Dixon et al 2000) or rat , even though the methods used in the above mentioned investigations to visualize autonomic nerves were different to those used in the present study.…”

Section: Discussionmentioning

confidence: 51%

The influence of botulinum toxin type A (BTX) on the immunohistochemical characteristics of noradrenergic and cholinergic nerve fibers supplying the porcine urinary bladder wall

Lepiarczyk¹,

Bossowska²,

Kaleczyc³

et al. 2011

Polish Journal of Veterinary Sciences

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Botulinum toxin (BTX) belongs to a family of neurotoxins which strongly influence the function of autonomic neurons supplying the urinary bladder. Accordingly, BTX has been used as an effective drug in experimental therapies of a range of neurogenic bladder disorders. However, there is no detailed information dealing with the influence of BTX on the morphological and chemical properties of nerve fibres supplying the urinary bladder wall. Therefore, the present study investigated, using double-labeling immunohistochemistry, the distribution, relative frequency and chemical coding of cholinergic and noradrenergic nerve fibers supplying the wall of the urinary bladder in normal female pigs (n=6) and in the pigs (n=6) after intravesical BTX injections. In the pigs injected with BTX, the number of adrenergic (DβH-positive) nerve fibers distributed in the bladder wall (urothelium, submucosa and muscle coat) was distinctly higher while the number of cholinergic (VAChT-positive) nerve terminals was lower than that found in the control animals. Moreover, the injections of BTX resulted in some changes dealing with the chemical coding of the adrenergic nerve fibers. In contrast to the normal pigs, in BTX injected animals the number of DβH/NPY-or DβH/CGRP-positive axons was higher in the muscle coat, and some fibres distributed in the urothelium and submucosa expressed immunoreactivity to CGRP. The results obtained suggest that the therapeutic effects of BTX on the urinary bladder might be dependent on changes in the distribution and chemical coding of nerve fibers supplying this organ.

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Section: Discussionmentioning

confidence: 51%

The influence of botulinum toxin type A (BTX) on the immunohistochemical characteristics of noradrenergic and cholinergic nerve fibers supplying the porcine urinary bladder wall

Lepiarczyk¹,

Bossowska²,

Kaleczyc³

et al. 2011

Polish Journal of Veterinary Sciences

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“…The literature dealing with the population of cholinergic nerve fibres supplying mammalian AGG based upon VAChT immunohistochemistry is very limited. The presence of cholinergic (VAChT-positive) fibres was shown in the human prostate and seminal vesicle (Dixon et al, 2000) and in the rat prostate (Schafer et al, 1998;Nadelhaft, 2003). Dixon et al (2000) found that the majority of VAChT-positive nerve endings localized beneath the epithelium of the human prostate and seminal vesicle contained also NPY whereas the small proportion stained for nitric oxide synthase (NOS).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, an alternative marker for cholinergic nerve structures has become available in the form of an antibody to vesicular acetylcholine transporter (VAChT -the protein associated with the storage of acetylcholine within vesicles in cholinergic nerve terminals). VAChT-positive nerve fibres were found in the human prostate and seminal vesicle (Dixon et al, 2000) and in the rat prostate (Schafer et al, 1998;Nadelhaft, 2003). However, the knowledge of neurochemical properties of cholinergic nerve fibres supplying AGG is still very limited.…”

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confidence: 99%

Immunohistochemical characterisation of cholinergic nerve fibres supplying accessory genital glands in the pig

Klimczuk¹,

Kaleczyc²,

Franke-Radowiecka³

et al. 2005

Vet. Med. - Czech

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Our previous immunohistochemical investigations revealed three major populations of nerve fibres supplying the porcine accessory genital glands (AGG) including noradrenergic, non-noradrenergic putative cholinergic and sensory nerve terminals (Kaleczyc et al., 1997). However, it is still unclear whether the non-noradrenergic nerve fibres are cholinergic in nature. The knowledge of the population of cholinergic nerve fibres in mammalian AGG based upon vesicular acetylcholine transporter (VAChT) immunohistochemistry is very limited. Therefore, the aim of the present investigation was to disclose the occurrence and colocalization patterns of VAChT, dopamineâ-hydroxylase (DβH) and some neuropeptides including vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and somatostatin (SOM) within nerve fibres supplying the porcine AGG. Double-immunohistochemical labellings showed that VAChT-positive nerve terminals were non-adrenergic (DâH-negative), however, many of them contained immunoreactivities to VIP, NPY and/or SOM. The coexistence patterns of these biologically active substances in nerve fibres supplying particular glands are similar but the density of cholinergic innervation varies between the organs. The innervation of the seminal vesicle and prostatic body is better developed than that of the disseminated part of the prostate and bulbourethral glands. The majority of cholinergic nerve fibres associated with blood vessels supplying the glands contain VIP and NPY and, to a lesser degree, SOM. The possible function and origin of the cholinergic nerve fibre population are discussed.

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“…The innervation of the prostate compartments close to the epithelium (periglandular and periductal) was more relevant for classifying the rats by age than interglandular (stromal) innervation; NPY and VIP were found particularly associated. At the present time, the peptidergic innervation of the prostate seems to play a role of special relevance in prostatic physiology (Dixon et al, 2000;Ventura et al, 2002). These neuropeptides, behaving as regulators of prostatic secretion, may be involved in modulation of the action of androgens (Gkonos et al, 1995;Juarranz et al, 2001;Ventura et al, 2002).…”

Section: Discussionmentioning

confidence: 97%

Evolution of Neuroendocrine Cell Population and Peptidergic Innervation, Assessed by Discriminant Analysis, During Postnatal Development of the Rat Prostate

Rodríguez¹,

Pozuelo²,

Alberca³

et al. 2011

Image Anal Stereol

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Serotonin immunoreactive neuroendocrine cells and peptidergic nerves (NPY and VIP) could have a role in prostate growth and function. In the present study, rats grouped by stages of postnatal development (prepubertal, pubertal, young and aged adults) were employed in order to ascertain whether age causes changes in the number of serotoninergic neuroendocrine cells and the length of NPY and VIP fibres. Discriminant analysis was performed in order to ascertain the classificatory power of stereologic variables (absolute and relative measurements of cell number and fibre length) on age groups. The following conclusions were drawn: a) discriminant analysis confirms the androgen-dependence of both neuroendocrine cells and NPY-VIP innervation during the postnatal development of the rat prostate; b) periglandular innervation has more relevance than interglandular innervation in classifying the rats in age groups; and c) peptidergic nerves from ventral, ampullar and periductal regions were more age-dependent than nerves from the dorso-lateral region.

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The distribution of vesicular acetylcholine transporter in the human male genitourinary organs and its co-localization with neuropeptide Y and nitric oxide synthase

Cited by 68 publications

References 21 publications

The influence of botulinum toxin type A (BTX) on the immunohistochemical characteristics of noradrenergic and cholinergic nerve fibers supplying the porcine urinary bladder wall

The influence of botulinum toxin type A (BTX) on the immunohistochemical characteristics of noradrenergic and cholinergic nerve fibers supplying the porcine urinary bladder wall

Immunohistochemical characterisation of cholinergic nerve fibres supplying accessory genital glands in the pig

Evolution of Neuroendocrine Cell Population and Peptidergic Innervation, Assessed by Discriminant Analysis, During Postnatal Development of the Rat Prostate

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