The distribution of Wolbachia pipientis strains in natural populations of Culex pipiens mosquitoes (Diptera: Culicidae)

Khrabrova, Natalia V.; Sibataev, A. K.

doi:10.1088/1755-1315/400/1/012012

Cited by 7 publications

(8 citation statements)

References 12 publications

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“…Previous studies have investigated the infection status of C. pipiens mosquitoes from various countries, including Russia, Kazakhstan, Kyrgyzstan, Belarus, Portugal, Spain, France, Italy, Switzerland, Belgium, U.K., the Netherlands, Greece, Turkey, Cyprus, Tunisia and Australia. They all confirmed a Wolbachia infection status of up to 100% (Vinogradova et al ., ; Duron et al ., ; Khrabrova et al ., ; Raharimalala et al ., ). In contrast, none of the larval samples identified as C. torrentium was infected.…”

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confidence: 99%

“…In contrast, none of the larval samples identified as C. torrentium was infected. This is in agreement with other studies analysing the prevalence of Wolbachia in C. torrentium mosquitoes, which did not report any positive populations (Khrabrova et al ., ; Raharimalala et al ., ). However, one study, which included two C. torrentium individuals from Italy, reported both of them to be positive for arthropod‐specific Wolbachia (Ricci et al ., ).…”

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confidence: 99%

“…As mentioned above, members of the Culex complex, namely C. pipiens biotype pipiens , C. pipiens biotype molestus and C. torrentium , are present in Germany (Rudolf et al ., ) and are susceptible to WNV as well as Sindbis virus infection (Lundstrom et al ., , ; Chiari et al ., ; Hesson et al ., ; Leggewie et al ., ). Earlier studies in which the presence of Wolbachia was determined showed that 80–100% of C. pipiens biotypes were infected (Rasgon & Scott, ; Duron et al ., ; Fedorova & Shaikevich, ; Khrabrova et al ., ; Zele et al ., ; Raharimalala et al ., ), but data on Wolbachia infection rates in C. torrentium are inconclusive. Studies conducted with Russian and Belgian populations of C. torrentium indicated that this species is not infected with Wolbachia (Fedorova & Shaikevich, ; Khrabrova et al ., ; Raharimalala et al ., ).…”

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confidence: 99%

“…Earlier studies in which the presence of Wolbachia was determined showed that 80–100% of C. pipiens biotypes were infected (Rasgon & Scott, ; Duron et al ., ; Fedorova & Shaikevich, ; Khrabrova et al ., ; Zele et al ., ; Raharimalala et al ., ), but data on Wolbachia infection rates in C. torrentium are inconclusive. Studies conducted with Russian and Belgian populations of C. torrentium indicated that this species is not infected with Wolbachia (Fedorova & Shaikevich, ; Khrabrova et al ., ; Raharimalala et al ., ). However, Wolbachia DNA sequences were found in two C. torrentium samples from Italy (Ricci et al ., ).…”

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Culex torrentium mosquitoes from Germany are negative for Wolbachia

Leggewie

Krumkamp

Badusche

et al. 2017

Medical Vet Entomology

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Abstract. Wolbachia (Rickettsiales: Anaplasmataceae) infects a wide range of arthropods, including several mosquito species. The bacterium is known to induce a plethora of phenotypes in its host, examples being the reproductive phenotype cytoplasmic incompatibility or resistance against infection with arboviruses. The latter is especially relevant when assessing the vector competence of mosquito species for emerging arboviruses. Thus, knowledge of Wolbachia infection status is important for the assessment of vector competence. To facilitate Wolbachia screening in mosquito populations, a quantitative polymerase chain reaction (qPCR) assay was developed to enable high-throughput analysis of mosquito samples. Using this assay, the Wolbachia infection status of the two most common Culex mosquito species in Germany, Culex pipiens biotype pipiens Linnaeus (Diptera: Culicidae) and Culex torrentium Martini (Diptera: Culicidae), was assessed. About 93% of all tested C. pipiens biotype pipiens individuals were positive for Wolbachia, whereas none of the C. torrentium samples was found to be infected. Furthermore, other applications of the qPCR assay were explored by assessing a potential link between the levels of Wolbachia and West Nile virus (WNV) infections in German C. pipiens biotype pipiens mosquitoes. No relationship was found between the two variables, indicating that a Wolbachia-induced antiviral phenotype in this mosquito population is not exclusively attributable to the general level of bacterial infection.

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Culex torrentium mosquitoes from Germany are negative for Wolbachia

Leggewie

Krumkamp

Badusche

et al. 2017

Medical Vet Entomology

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“…p. molestus was only infected by one strain of W. pipientis but Cx. p. pipiens by two different strains 34 . Moreover, that the specimens of Cx.…”

Section: For Example W Pipientis Infection Significantly Reduced Hymentioning

confidence: 99%

Genomic differentiation and intercontinental population structure of mosquito vectorsCulex pipiens pipiensandCulex pipiens molestus

Yurchenko

Masri²,

Khrabrova

et al. 2019

Preprint

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AbstractUnderstanding the population structure and mechanisms of taxa diversification is important for organisms responsible for the transmission of human diseases. Two vectors of West Nile virus, Culex pipiens pipiens and Cx. p. molestus, exhibit epidemiologically important behavioral and physiological differences, but the whole-genome divergence between them was unexplored. In this study, we re-sequenced and compared the whole genomes of 40 individual mosquitoes from four locations in Eurasia and North America: the Republic of Belarus, the Kyrgyz Republic, and the United States of America. Principal Component, ADMIXTURE, and neighbor joining analyses of the nuclear genomes identified two intercontinental, monophyletic clusters of Cx. p. pipiens and Cx. p. molestus. The third cluster, having a polyphyletic origin, was formed by Cx. p. pipiens and Cx. quinquefasciatus from the USA. The level of genomic differentiation between the subspecies was uniform along chromosomes. The ADMIXTURE analysis determined signatures of admixture in all Cx. p. pipens populations but not in Cx. p. molestus populations. Comparison of mitochondrial genomes among the specimens showed a paraphyletic origin of the major haplogroups between the subspecies but a monophyletic structure between the continents. Thus, our study identified that Cx. p. molestus and Cx. p. pipiens represent different evolutionary units with monophyletic origin that have undergone incipient ecological speciation.