The diversity of citrus endophytic bacteria and their interactions with Xylella fastidiosa and host plants

Azevedo, João Lúcio de; Araújo, Welington Luiz; Lacava, Paulo Teixeira

doi:10.1590/1678-4685-gmb-2016-0056

Cited by 41 publications

(23 citation statements)

References 137 publications

(199 reference statements)

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“…Considering the unique microbial communities found for each olive genotype, however, no Bacteroidetes could be isolated from "Arbequina," while for "Picual," unique bacteria belonging to Actinobacteria phylum were isolated. These results are in line with the gross taxonomic bacterial distribution of isolates already identified in the olive rhizosphere and phyllosphere (Ercolani, 1991;Aranda et al, 2011), as well as with the composition of bacterial endophytes identified in other woody plants, such as citrus, grapevine, or poplar (Bell et al, 1995;Compant et al, 2011;Azevedo et al, 2016;Durand et al, 2018). At the genus level, Sphingomonas, Methylobacterium, Curtobacterium, Frigoribacterium, and Bacillus were among the most abundant culturable genera.…”

Section: Discussionsupporting

confidence: 86%

Culture-Dependent and Culture-Independent Characterization of the Olive Xylem Microbiota: Effect of Sap Extraction Methods

et al. 2020

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Microbial endophytes are well known to protect host plants against pathogens, thus representing a promising strategy for the control of xylem-colonizing pathogens. To date, the vast majority of microbial communities inhabiting the olive xylem are unknown; therefore, this work pursues the characterization of the xylem-limited microbiome and determines whether the culture isolation medium, olive genotype, and the plant material used to analyze it can have an effect on the bacterial populations retrieved. Macerated xylem tissue and xylem sap extracted with the Scholander chamber from olive branches obtained from two cultivated and a wild olive genotypes were analyzed using culturedependent and-independent approaches. In the culture-dependent approach using four solid culture media, a total of 261 bacterial isolates were identified after performing Sanger sequencing of 16S rRNA. Culturable bacteria clustered into 34 genera, with some effect of culture media for bacterial isolation. The cultivated bacteria belonged to four phyla and the most abundant genera included Frigoribacterium (18.8%), Methylobacterium (16.4%), and Sphingomonas (14.6%). On the other hand, in the culture-independent approach conducted using Illumina MiSeq 16S rRNA amplicon sequencing [next-generation sequencing (NGS)] of the xylem extracts, we identified a total of 48 operational taxonomic units (OTUs) belonging to five phyla, being Sphingomonas (30.1%), Hymenobacter (24.1%) and Methylobacterium (22.4%) the most representative genera (>76% of reads). In addition, the results indicated significant differences in the bacterial communities detected in the xylem sap depending on the genotype of the olive tree studied and, to a minor extent, on the type of sap extraction method used. Among the total genera identified using NGS, 14 (41.2%) were recovered in the culture collection, whereas 20 (58.8%) in the culture collection were not captured by the NGS approach. Some of the xylem-inhabiting bacteria isolated are known biocontrol agents of plant pathogens, whereas for others little information is known and are first reported for olive. Consequently, the potential role of these bacteria in conferring olive tree protection against xylem pathogens should be explored in future research.

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Section: Discussionsupporting

confidence: 86%

Culture-Dependent and Culture-Independent Characterization of the Olive Xylem Microbiota: Effect of Sap Extraction Methods

et al. 2020

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“…However, there are also reports showing the biocontrol potential of some members of this genus. For example, C. flaccumfaciens revealed to inhibit the phytopathogens Xyllela fastidiosa 18,64 and V. dahliae 65 . Arthrobacter includes a large number of widespread species, in particular in soil, and with great importance in environmental and industrial applications [66][67][68] .…”

Section: Discussionmentioning

confidence: 99%

Impact of plant genotype and plant habitat in shaping bacterial pathobiome: a comparative study in olive tree

Mina

Pereira

Lino-Neto

et al. 2020

Sci Rep

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Plant-inhabiting microorganisms interact directly with each other affecting disease progression.However, the role of host plant and plant habitat in shaping pathobiome composition and their implication for host susceptibility/resistance to a particular disease are currently unknown. For the elucidation of these questions, both epiphytic and endophytic bacterial communities, present in asymptomatic and symptomatic twigs from olive cultivars displaying different susceptibilities to olive knot (OK) disease, were investigated using culturing methods. OK disease was the main driver of the bacterial community, causing changes on their diversity, abundance and composition. OK disease effect was most notorious on OK-susceptible cultivar and when considering the endophytic communities. plant habitat (epiphytes vs. endophytes) also contributed to the bacterial community assembling, in particular on symptomatic twigs (knots) of OK-susceptible cultivar. In contrast, host cultivar had little effect on the bacterial community composition, but OK-symptomatic twigs (knots) revealed to be more affected by this driver. Overall, the pathobiome seems to result from an intricate interaction between the pathogen, the resident bacteria, and the plant host. Specific bacterial genera were associated to the presence or absence of OK disease in each cultivar. Their ability to trigger and/or suppress disease should be studied in the future.

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“…Xylella fastidiosa (Gammaproteobacteria) is known as a pest of ornamental and economic trees the USA and Taiwan (Sherald & Kostka, ), moved by sap‐sucking insects. Since 2013 several subspecies have become serious economic pests of Olea europaea and citrus trees in Europe (Azevedo, Araujo, & Lacava, ; White, Bullock, Hooftman, & Chapman, ), plus infecting species of Quercus , Platanus and Ulmus , including U. glabra where it causes bacterial leaf scorch (Sherald & Kostka, ). Ulmus species are affected by the subspecies ‘multiplex’ of X. fastidiosa (Baldi & La Porta, ).…”

Section: Herbivory and Diseasementioning

confidence: 99%

Biological Flora of the British Isles: Ulmus glabra

2018

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This account presents information on all aspects of the biology of Ulmus glabraHudson (wych elm) that are relevant to understanding its ecological characteristics and behaviour. The main topics are presented within the standard framework of the Biological Flora of the British Isles: distribution, habitat, communities, responses to biotic factors, responses to environment, structure and physiology, phenology, floral and seed characters, herbivores and disease, history and conservation.2. Ulmus glabra is a large forest tree, and often an important canopy tree in ancient and semi-natural woodlands. It is primarily native to the north and west of Britain and much of mainland Europe. It is the only elm native to Ireland. It is the most distinct of the British elms in that it rarely suckers and sets abundant viable seed.Although found on limestone screes and cliffs, and hedgerows, it is primarily a woodland tree, especially on moist, basic soils. In many secondary woodlands, it often co-occurs with Acer pseudoplatanus and has ecological needs that are similar to Fraxinus excelsior. 3.Ulmus glabra has clusters of c. 25 hermaphrodite flowers appearing before the leaves on previous year's growth. Seeds are wind-dispersed, falling in April to July, but remain viable for only a few days. Nevertheless, seedling establishment can be abundant. Hybridisation with other northern European elms is common but hybrids are notoriously difficult to identify and therefore probably under-recorded.4. The health and survival of wych elm in Europe has been seriously compromised since the 1970s due to Dutch elm disease caused by the fungus Ophiostoma novoulmi, transmitted by elm bark beetles (Scolytus spp.). To the south of its Scottish stronghold, many elms are reduced to small trees regrowing from basal sprouts or seeds. These trees tend to be reinfected once trunk diameter exceeds 10 cm.Fortunately for its long-term survival, seed production usually begins a number of years before they are reinfected.

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The diversity of citrus endophytic bacteria and their interactions with Xylella fastidiosa and host plants

Cited by 41 publications

References 137 publications

Culture-Dependent and Culture-Independent Characterization of the Olive Xylem Microbiota: Effect of Sap Extraction Methods

Culture-Dependent and Culture-Independent Characterization of the Olive Xylem Microbiota: Effect of Sap Extraction Methods

Impact of plant genotype and plant habitat in shaping bacterial pathobiome: a comparative study in olive tree

Biological Flora of the British Isles: Ulmus glabra

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