The division between fast- and slow-growing species corresponds to natural relationships among the mycobacteria

Stahl, David A.; Urbance, John W.

doi:10.1128/jb.172.1.116-124.1990

Cited by 267 publications

(200 citation statements)

References 50 publications

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“…Several of these organisms, including M. haemophilum, Mycobacterium shimoidei, and Mycobacterium ulcerans, are considered pathogenic for humans. We compared our results with the comprehensive overview provided by the 16s rRNA phylogenetic tree of the RDP, as well as with the results of specific analyses described previously (13,29,31,36). For the most part, our results corroborate the 16s rRNA tree data, although there are some exceptions.…”

Section: Discussionsupporting

confidence: 78%

“…This finding is not consistent with the data of Stahl and Urbance (36), who found that M. chitae is less closely related to the genus Mycobacterium than Rhodococcus equi is. We found that Mycobacterium fallax and Mycobacterium poriferae were separated from the main group of the genus Mycobacterium, whereas on the 16s rRNA trees M. fallax clusters with M. chitae and Mycobacterium gadium.…”

Section: Resultscontrasting

confidence: 57%

“…In addition, there are several subspecies and many trivially named strains. Members of this genus are frequently categorized as being slow growers (more than 7 days is required to see colonies on a plate) or fast growers (less than 7 days is required) (36). Several important human pathogens, including Mycobacterium tuberculosis and Mycobacterium leprue, are members of the slowly growing group, as are a number of opportunistic pathogens (Table 1) (15).…”

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confidence: 99%

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Comparison of Mycobacterium 23S rRNA Sequences by High-Temperature Reverse Transcription and PCR

Stone

Nietupski

Breton

et al. 1995

International Journal of Systematic Bacteriology

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We describe a modified rRNA sequence analysis method which we used to determine the phylogenetic relationships among 58 species belonging to the genus Mycobacterium. We combined the sensitivity of the reverse transcriptase PCR for amplifying nanogram amounts of template rRNA material with the elevated extension temperatures used for the thermostable DNA polymerase from Thermus thermophilus. A 70°C reverse transcription extension step permitted improved read-through of highly structured rRNA templates from members of the genus Mycobacterium, which have G+C contents of 66 to 71 mol%. The nucleic acid sequences of the amplified material were then determined by performing thermal cycle sequencing with a-"P-labeled primers, again with extension at 70°C. Nonspecifically terminated bands were chased by using terminal deoxynucleotidyl transferase. Our method had a template requirement of nanogram amounts or less of purified RNA or 2,000 CFU of intact cells and had sufficient sensitivity so that lyophils obtained from the American Type Culture Collection could be used as source material. Sequences from a 250-nucleotide stretch of the 23s rRNA were aligned, and phylogenetic trees were evaluated by using the De Soete distance treeing algorithm and Rhodococcus bronchialis as the outgroup. Our 23s rRNA trees were compared with previously published 16s rRNA trees, including the comprehensive trees developed by the University of Illinois Ribosomal Database Project, and included 15 species not evaluated previously. Most of the groups were in general agreement and were consistent with relationships determined on the basis of biochemical characteristics, but some new relationships were also observed.

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Section: Discussionsupporting

confidence: 78%

Section: Resultscontrasting

confidence: 57%

mentioning

confidence: 99%

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Comparison of Mycobacterium 23S rRNA Sequences by High-Temperature Reverse Transcription and PCR

Stone

Nietupski

Breton

et al. 1995

International Journal of Systematic Bacteriology

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“…The presence of MPTR-homologous DNA among these mycobacterial species suggests an evolutionary relationship among these mycobacterial species. This is consistent with the phylogenetic relationship of these species based on the homology of the 16S rRNA sequences as described by Stahl and Urbance (34). Surprisingly, although the M. avium complex bacteria belong to this group of taxonomically closely related mycobacteria (on the basis of 16S rRNA homology), they do not contain MPTR-homologous DNA.…”

Section: Wsupporting

confidence: 74%

Characterization of a major polymorphic tandem repeat in Mycobacterium tuberculosis and its potential use in the epidemiology of Mycobacterium kansasii and Mycobacterium gordonae

1992

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In this study, the occurrence of repeated DNA sequences in the chromosome of Repetitive DNA sequences have also been found in mycobacteria. The insertion elements IS900 from Mycobacterium paratuberculosis (4, 12), IS901 from Mycobacterium avium (19), IS1096 from Mycobacterium smegmatis (2), IS1081 from Mycobacterium bovis, and the IS3 family of insertion elements present in the species of the Mycobacterium tuberculosis complex (15,22,37) are present in multiple copies in the mycobacterial chromosome (16,40). Additionally, unidentified repeated sequences, or repeats which do not share characteristics with other sequences, have been found in Mycobacterium leprae (3, 13, 43) and M. tuberculosis (6, 14, 15, 27).The mycobacterial repeated DNA sequences described above were selected from gene libraries on the basis of their species specificity or differences in hybridization to particular DNA probes. However, no systematic study has been done on the major classes of repetitive DNA in mycobacteria.In this study, we investigated systematically the occurrence of repetitive DNA in M. tuberculosis. We show that a major repetitive DNA in this species belongs to a family consisting of short tandemly repeated sequences of 10 bp, separated by 5-bp spacer DNA. Furthermore, we analyzed the presence of this DNA family in other mycobacterial species and the potential use of this repetitive DNA for * Corresponding author.subtyping of mycobacterial strains and the epidemiology of mycobacterioses.

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“…Members of the M . tuberculosis complex are also very closely related (98% similarity in 16s rRNA sequences) to other slow-growing mycobacteria (Rogall et al, 1990;Stahl & Urbance, 1990;Cox et al, 1991). This high degree of similarity in sequence suggests that the principal features of the leader region of the M .…”

Section: Discussionmentioning

confidence: 78%

The nucleotide sequence of the promoter, 16S rRNA and spacer region of the ribosomal RNA operon of Mycobacterium tuberculosis and comparison with Mycobacterium leprae prefnsor rRNA

Kempsell¹,

Estrada

et al. 1992

Journal of General Microbiology

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Mycobacterium tuberculosis H37Rv has a single rrn (ribosomal RNA) operon. The operon was cloned and a region of 1536 nucleotides was sequenced, starting 621 bp upstream from the 5'-end of the 16s rRNA coding region and continuing to the start of the 23s rRNA coding region. The 16s rRNA sequence inferred from the gene sequence was found to differ in one position from Mycobacterium bovis (nucleotide 1443) and from Mycobacterium microti (nucleotide 427). A single putative promoter was identified on the basis of similarities with the sequence of rrn operons of Bacillus subtilis and Escherichia culi. The regions of similarity include a -35 box, a -10 box, a stringent response element, antitermination signals, potential RNAase I11 processing sites and features of precursor rRNA secondary structure. Sequences upstream from the 5'-end of Mycobacterium leprae 1 6 s rRNA were also investigated. Homologous schemes of secondary structure were deduced for precursor rRNA of both M. tuberculosis and M. Zeprae; although the principal features are common to both species there are notable differences.

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The division between fast- and slow-growing species corresponds to natural relationships among the mycobacteria

Cited by 267 publications

References 50 publications

Comparison of Mycobacterium 23S rRNA Sequences by High-Temperature Reverse Transcription and PCR

Comparison of Mycobacterium 23S rRNA Sequences by High-Temperature Reverse Transcription and PCR

Characterization of a major polymorphic tandem repeat in Mycobacterium tuberculosis and its potential use in the epidemiology of Mycobacterium kansasii and Mycobacterium gordonae

The nucleotide sequence of the promoter, 16S rRNA and spacer region of the ribosomal RNA operon of Mycobacterium tuberculosis and comparison with Mycobacterium leprae prefnsor rRNA

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