The Division of Neuronal Progenitor Cells during Migration in the Neonatal Mammalian Forebrain

Menezes, João R.L.; Smith, Cyndi M.; Nelson, Kasey C.; Luskin, Marla B.

doi:10.1006/mcne.1995.0002

Cited by 256 publications

(208 citation statements)

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“…Indeed, many species have Dcx+ cells in the adult brain, and these Dcx+ cells may be generated embryonically (Supplementary information, Figure S2) [24,29,30]. If Dcx+ cells in the adult human SVZ and RMS are bona fide young migrating neuroblasts, there should be at least a small number of Dcx+ cells capable of proliferating in vivo, which is typical of many neuroblasts in the adult rodent and monkey brain ( Figure 1O-1S') [9,10,28,31]. We first did an extensive analysis of the proliferating cells in the adult human SVZ and RMS.…”

Section: Identification and Characterization Of Neuroblasts In The Admentioning

confidence: 99%

Identification and characterization of neuroblasts in the subventricular zone and rostral migratory stream of the adult human brain

et al. 2011

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It is of great interest to identify new neurons in the adult human brain, but the persistence of neurogenesis in the subventricular zone (SVZ) and the existence of the rostral migratory stream (RMS)-like pathway in the adult human forebrain remain highly controversial. In the present study, we have described the general configuration of the RMS in adult monkey, fetal human and adult human brains. We provide evidence that neuroblasts exist continuously in the anterior ventral SVZ and RMS of the adult human brain. The neuroblasts appear singly or in pairs without forming chains; they exhibit migratory morphologies and co-express the immature neuronal markers doublecortin, polysialylated neural cell adhesion molecule and βIII-tubulin. Few of these neuroblasts appear to be actively proliferating in the anterior ventral SVZ but none in the RMS, indicating that neuroblasts distributed along the RMS are most likely derived from the ventral SVZ. Interestingly, no neuroblasts are found in the adult human olfactory bulb. Taken together, our data suggest that the SVZ maintains the ability to produce neuroblasts in the adult human brain.

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Section: Identification and Characterization Of Neuroblasts In The Admentioning

confidence: 99%

Identification and characterization of neuroblasts in the subventricular zone and rostral migratory stream of the adult human brain

et al. 2011

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“…While en route to the OB, a vast number of cells are dividing from the SVZ to the OB (Menezes et al, 1995). Accordingly, we selected four rostrocaudal levels along the RMS, as described in Figure 4 A, from the emergence of the stream (Fig.…”

Section: Increased Number Of Rapidly Proliferating Cells In Svz Of Mumentioning

confidence: 99%

Increased Neurogenesis in Adult mCD24-Deficient Mice

2002

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mCD24, a glycosylphosphatidylinositol-anchored highly glycosylated molecule, is expressed on differentiating neurons during development. In the adult CNS, its expression is restricted to immature neurons located in two regions showing ongoing neurogenesis: the subventricular zone (SVZ) of the lateral ventricle pathway and the dentate gyrus (DG) of the hippocampal formation. Here, combining bromodeoxyuridine (BrdU) and proliferating cell nuclear antigen labelings we confirmed that mCD24 is expressed on proliferating cells. To determine whether the inactivation of the molecule may affect adult neurogenesis, we analyzed the phenotype of mCD24-deficient mice (mCD24Ϫ/Ϫ). We labeled cells in S-phase with a pulse, a long, or a cumulative administration of BrdU and analyzed cells in different zones according to their dividing rate (rapid and slow) both in the control and mCD24Ϫ/Ϫ. We found a significant increase in the number of rapid (in the SVZ and the DG) and slow (in the SVZ) proliferating cells. Cumulative assays revealed a global reduction of the total cell cycle duration of rapidly proliferating precursors of SVZ. We investigated the fate of supernumerary cells and observed an increased number of apoptotic cells (terminal deoxynucleotidyl transferasemediated biotinylated UTP nick end labeling) in the mutant SVZ. Furthermore, we found no difference in the size of the olfactory bulb between wild-type (WT) and mutant mice. In support, mCD24 deletion did not appear to affect migration in the migratory stream. A comparison of the organization of migrating precursors between WT and mCD24 Ϫ/Ϫ, both in vivo at the optic and electron microscopic levels and in SVZ cultured explants, did not show any changes in the arrangement of neuroblasts in chain-like structures.Altogether, our data suggest that mCD24 regulates negatively cell proliferation in zones of secondary neurogenesis.

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“…were made at an interval of 23 h and 1 h after the second injection the rats were perfused as described above. The incorporated BrdU was detected by immunohistochemistry following an acid wash, 43 using a monoclonal antibody (RPN 202, Amersham), and developed with an anti-mouse ABC kit (Vector) using di-aminobenzidine as chromogen.…”

Section: Detection Of Proliferating Cellsmentioning

confidence: 99%

Evidence that the bifunctional redox factor / AP endonuclease Ref-1 is an anti-apoptotic protein associated with differentiation in the developing retina

et al. 2000

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The Division of Neuronal Progenitor Cells during Migration in the Neonatal Mammalian Forebrain

Cited by 256 publications

References 0 publications

Identification and characterization of neuroblasts in the subventricular zone and rostral migratory stream of the adult human brain

Identification and characterization of neuroblasts in the subventricular zone and rostral migratory stream of the adult human brain

Increased Neurogenesis in Adult mCD24-Deficient Mice

Evidence that the bifunctional redox factor / AP endonuclease Ref-1 is an anti-apoptotic protein associated with differentiation in the developing retina

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