The DNA sequence of an IS<i>1</i>‐flanked transposon coding for resistance to chloramphenicol and fusidic acid

Marcoli, Roberto; Iida, Shigeru; Bickle, Thomas A.

doi:10.1016/0014-5793(80)80011-1

Cited by 91 publications

(42 citation statements)

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“…A study of the cat of pC221 was also a preliminary step in the larger task of understanding the structure and functions of pC221 and part of an ongoing analysis of the evolution of the CAT 'family' of genes and their products [l], the best-known example of which is the gene for the type I enzyme variant encoded by Tn9 [15] and pBR325 [16]. Although subject to catabolite repression mediated at the transcriptional level by cyclic AMP [ 17,181, expression of the cat of Tn9 is constitutive and is not responsive to chloramphenicol.…”

Section: Introductionmentioning

confidence: 99%

Chloramphenicol acetyltransferase gene of staphylococcal plasmid pC221

et al. 1985

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The nucleotide sequence of the inducible chloramphenicol acetyltransferase gene (cat) of Staphylococcus aureus plasmid pC22 1 has been determined. The deduced primary structure for the 2 15 residue polypeptide (25.9 kDa) is in agreement with partial amino acid sequence data on the purified protein, previously designated as the type C variant of CAT. In common with the inducible cat elements of pC194 and B. pumilus, the 5' non-coding region of the cat of pC221 contains an inverted complementary repeat ('stem-loop' or 'hairpin') which may sequester the predicted ribosome bonding site of the mRNA. The likely transcription initiation site has been determined in vitro using purified B. subtilis RNA polymerase. Recombinant plasmids carrying the cat of pC221 on a 1156 bp TaqI fragment are expressed inefflciently in Escherichia coli, wherein induction is both poor and orientation-specific.

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Section: Introductionmentioning

confidence: 99%

Chloramphenicol acetyltransferase gene of staphylococcal plasmid pC221

et al. 1985

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“…Cm sensitive derivatives of PZCm67 PI Cm phages carrying Cm transposons flanked by directly repeated IS2 elements segregate PICmS derivatives (Iida & Arber, 1977;1980). Recombination between the IS2 sequences results in the loss of the Cm segment, leaving one copy of IS2 at the insertion site of the Cm transposon.…”

Section: Resultsmentioning

confidence: 99%

“…Sequencing revealed the DNA between these two IS2 elements to consist of 77 bp (data not shown). This DNA must have originated from the Cmr segment next to ISZc, since the sequence of the ISZc end and the adjacent 77 bp is identical to the sequence carried on a related Cm transposon derived from Tn267Z (Marcoli et al, 1980). Therefore, the deletion was caused by ISZb and removed all the coding sequences of the Cmr (cat) gene together with its promoter (Le Grice et al, 1982).…”

Section: Resultsmentioning

confidence: 99%

“…Like ISZ-flanked Cm transposons (Iida & Arber, 1977, 1980MacHattie & Jackowski, 1977;Meyer & Iida, 1979), the ISZ-77 bp-IS2 structure is relatively stable even in rec+ cells. Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The wild-type phage DNA present in the virions is double-stranded, circularly permuted and has a terminal redundancy of about 9 kb. A drug resistance transposon, shorter than the terminal redundancy, inserted into a non-essential region of the P1 genome yields a plaqueforming, specialized transducing phage for the drug resistance marker (Iida & Arber, 1977, 1980. P1 DNA carries one copy of an IS1 sequence as a natural constituent which does not affect essential phage functions (Iida et al, 1978;Meyer et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

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Bacteriophage P1 Derivatives Unaffected in Their Growth by a Large Inversion or by IS Insertions at Various Locations

Iida¹,

Meyer²,

Arber³

1985

Microbiology

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129Several plaque-forming phage P1 derivatives carrying DNA rearrangements associated with IS elements are described. They have ISl, IS3 and IS5 inserted in four distinct locations, all of which are non-essential regions for phage P1 propagation. One derivative carries a genome segment, inverted relative to the one in the P1 wild-type genome, between two inverted copies of ISl. The inverted DNA segment spans about 23 kb of the 90 kb long P1 genome and it includes the invertible C segment. This phage is as viable as an isomeric P1 which carries the relevant segment in its original orientation. These results are discussed with regard to the genome organization of phage P1.

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Phenotypic reversion of an IS1-mediated deletion mutation: a combined role for point mutations and deletions in transposon evolution.

Lida¹,

Marcoli²,

Bickle³

1982

The EMBO Journal

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We have physically characterised a deletion mutant of the R plasmid R100 which has lost all of the antibiotic resistances, including chloramphenicol resistance (Cmr), coded by its IS1‐flanked r‐determinant. The deletion was mediated by one of the flanking IS1 elements and terminates within the carboxyl terminus of the Cmr gene. DNA sequence analysis showed that the mutated gene would produce a protein 20 amino acids longer than the wild‐type due to fusion with an open reading frame in the IS element. Surprisingly for a deletion mutation, rare, spontaneous Cmr revertants could be recovered. Two of the four revertants studied had frame shifts due to the insertion of a single AT base pair at the same position; the revertants would code for a protein five amino acids shorter than the wild‐type. The other two revertants had acquired duplications of the 34‐bp inverted terminal repeat sequences of the IS1 element and would direct the synthesis of a protein six amino acids longer than the wild‐type. The reverted Cmr markers were still capable of transposition. These observations suggest a role for point mutations and small DNA rearrangements in the formation of new gene organisations produced by mobile genetic elements.

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The DNA sequence of an IS1‐flanked transposon coding for resistance to chloramphenicol and fusidic acid

Cited by 91 publications

References 14 publications

Chloramphenicol acetyltransferase gene of staphylococcal plasmid pC221

Chloramphenicol acetyltransferase gene of staphylococcal plasmid pC221

Bacteriophage P1 Derivatives Unaffected in Their Growth by a Large Inversion or by IS Insertions at Various Locations

Phenotypic reversion of an IS1-mediated deletion mutation: a combined role for point mutations and deletions in transposon evolution.

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