The Drosophila melanogaster sex-peptide: A molecular analysis of structure-function relationships

Schmidt, Thomas; Choffat, Yves; Klauser, Stephan; Kubli, Eric

doi:10.1016/0022-1910(93)90023-k

Cited by 101 publications

(138 citation statements)

References 21 publications

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“…Thus, once in the rspb.royalsocietypublishing.org Proc R Soc B 280: 20131938 haemolymph, SP seems to be delivered to a dedicated set of neurons. Ours and previous SP injection experiments have shown that both receptivity and oviposition are altered at the same critical concentration [42]. An interpretation of these results would be the presence of an SP receptor on a single type of neuron, which then induces the postmating switch by signalling to higher order processing centres.…”

Section: (C) Routes Of Sex-peptide To Its Targetssupporting

confidence: 66%

Multiple pathways mediate the sex-peptide-regulated switch in female Drosophila reproductive behaviours

et al. 2013

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Male-derived sex-peptide (SP) induces profound changes in the behaviour of Drosophila females, resulting in decreased receptivity to further mating and increased egg laying. SP can mediate the switch in female reproductive behaviours via a G protein-coupled receptor, SPR, in neurons expressing fruitless, doublesex and pickpocket. Whether SPR is the sole receptor and whether SP induces the postmating switch in a single pathway has not, to our knowledge been tested. Here we report that the SP response can be induced in the absence of SPR when SP is ectopically expressed in neurons or when SP, transferred by mating, can access neurons through a leaky blood brain barrier. Membrane-tethered SP can induce oviposition via doublesex, but not fruitless and pickpocket neurons in SPR mutant females. Although pickpocket and doublesex neurons rely on G(o) signalling to reduce receptivity and induce oviposition, G(o) signalling in fruitless neurons is required only to induce oviposition, but not to reduce receptivity. Our results show that SP's action in reducing receptivity and inducing oviposition can be separated in fruitless and doublesex neurons. Hence, the SP-induced postmating switch incorporates shared, but also distinct circuitry of fruitless, doublesex and pickpocket neurons and additional receptors.

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Section: (C) Routes Of Sex-peptide To Its Targetssupporting

confidence: 66%

Multiple pathways mediate the sex-peptide-regulated switch in female Drosophila reproductive behaviours

et al. 2013

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“…1) [8,9]. The Hyp was shown to be (2S,4R)-4-hydroxyproline by comparison to a synthetic standard [9], and is consistent with the ability of all known prolyl-4-hydroxylases, including that from D. melanogaster, to incorporate the hydroxyl group in the R-configuration. The biological functions of SP appear to be mediated by specific parts of the peptide [6].…”

Section: Introductionsupporting

confidence: 68%

NMR studies of the solution conformation of the sex peptide from Drosophila melanogaster

2011

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a b s t r a c tThe insect sex peptide (SP) elicits a variety of biological responses upon transfer to the mated female. SP contains 36 amino acids, including a tryptophan-rich N-terminal region, a central region containing five hydroxyproline (Hyp) residues, and a C-terminal region enclosed by a disulfide bridge. The solution structure of SP, studied here using NMR spectroscopy, includes a motif WPWN that adopts a type I b-turn in the N-terminal Trp-rich region. This turn region is connected to the central Hyp-rich region, which adopts extended and/or PPII-like conformations. The C-terminal disulfide-bonded loop populates helical turns or nascent helical structure. Overall, the results reveal a rather flexible peptide that lacks a compact folded structure in solution.

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“…Addition of nonradioactive iodine ( 127 I) does not affect the biological activity. tion of 0.6 pmol SP/female (Kubli, 1996;Schmidt et al, 1993). Both findings suggest that a common receptor is present at the top of the SP response cascade.…”

Section: Figurementioning

confidence: 78%

“…Analysis of structure-function relationships has shown that the C-terminal part of SP is essential to elicit oviposition and reduced receptivity (Schmidt et al, 1993). SP fragments elicit either both or none of the postmating responses.…”

mentioning

confidence: 99%

Binding sites of Drosophila melanogaster sex peptide pheromones

Ottiger¹,

Soller²,

Stocker³

et al. 2000

J. Neurobiol.

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The Drosophila melanogaster sex-peptide: A molecular analysis of structure-function relationships

Cited by 101 publications

References 21 publications

Multiple pathways mediate the sex-peptide-regulated switch in female Drosophila reproductive behaviours

Multiple pathways mediate the sex-peptide-regulated switch in female Drosophila reproductive behaviours

NMR studies of the solution conformation of the sex peptide from Drosophila melanogaster

Binding sites of Drosophila melanogaster sex peptide pheromones

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